R-Phycoerthyrin (R-RPE) Streptavidin Conjugate designed for Lateral Flow
Our Streptavidin R-Phycoerythrin conjugates are manufactured from a red algae and the first of its kind protein based fluorescent particles specifically desinged for lateral flow applications. R-RPE is a highly absorptive fluorescent molecule that has excellent detectability. It is the fluorochrome of choice when the brightest signal is needed and is therefore used most often when high sensitivity is essential for detectability and/or accuracy.
Consistent lot-to-lot performance resulting from continuous culture of source organisms and high purity. – Very high water solubility – Homogeneous structure with defined molecular weights – Total control on growth conditions and nutrition, which avoids contamination from extraneous organisms and waste found in the open ocean. Proteins are harvested at the optimal stage of the growth cycle to assure uniform product characteristics. The pigment is extracted and stabilized within minutes of harvest, virtually eliminating risks from the action of proteases.
250ul stock ready to use for lateral flow
50mL lateral flow running buffer designed for Streptavidin R-Phycoerythrin particles
Excitation max: 566nm
Emission max: 575nm
Other Products
TD4Z Mini Centrifuge – Vortex
Product Info
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Product Info
Product Highlights
Speed: Adjustable speed, up to 4500 rpm
Vortex Oscillation Intensity:1-4 intensity levels, selectable and adjustable
Programming:Programmable,freely choose combinations of oscillation strength,duration.centrifugation speed, and time
Safety:Safety interlock device to ensure user safety
Compatibility: Single tube or multi-tube centrifugation/mixing with one-button operation, saving time and cost
Display:Touch buttons and an LCD screen clearly indicate and set oscillation level, duration, and cycle count
Convenience: Multi-functional, compact design, occupies minimal space
Technical Specifications
Dimensions (mm)
200(w)x243(H)x127(D)
Working temperature
5℃ to 40℃
Oscillation Duration
0-30 seconds (increment of 1 second)
TD4Z Speed range
1000-3600 rpm (increment of 100 rpm)
Power Supply
AC 220V 50/60 Hz
Spin Timer
0 seconds to 30 minutes
Weight
3.5kg (including power supply)
Oscillation Intensity
Level 1-4
Speed range TD4Z
1-99 programs
Centrifugation Adjustment TD4Z
1000-3600 rpm (increment of 100 rpm)
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PCR laboratories and general molecular biology laboratories Model: TD4Z
Usages: For cultivating, enumerating and preserving nonfastidious bacteria.
Principle: Peptone and beef extract powder to provide nitrogen, vitamins, amino acids and carbon; agar as medium coagulant.
Formulation(per liter): Peptone: 5g Beef extract :3g Agar :15g Final pH 6.8 ± 0.2
How to use: 1. Suspend 23g of product, adding 1L of distilled or deionized water, heated to boiling stirring until completely dissolved, dispensing into flask, autoclave at 121 ℃ for 15min, set aside. 2.Diluted and treated samples.
Quality control：
Item
The name and number of strain
Growth
Colony Color
1
Escherichia coli ATCC25922
Good
Dark red
2
Aerogenes CMCC (B) 45103
Good
red
3
Staphylococcus aureus ATCC6538
Suppressed
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Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
The series of DNA Size Selection Kits (Magnetic Beads) were developed for DNA size selection using magnetic beads. A total of 11 kits are available, with different selection ranges spanning from 50 bp to over 10 kb. The kits provide a simple and quick approach for the enrichment of a specific range of DNA fragments. The kit workflow allows double-sided or single-sided size selection for specific size cutoffs.
Gel images of different ranges of size selection. Sheared human genomic DNA was used as input.
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DNA size selection is a selective capture of DNA fragments of a specific range of size for next-generation sequencing (NGS) library preparations, PCR, ChIP assay, DNA ligations, endonuclease digestions, adapter removal, and other genomics and molecular biology applications. DNA size selection is preferred after NGS library prep in most of the cases. The NGS library preparation is related to the quality of the sequencing data. Precise NGS library size selection can increase sequencing efficiency, improve data quality, and reduce costs.
There are two types of sequencing technologies: short-read sequencing and long-read sequencing. Short-read sequencing uses DNA libraries that contain small insert DNA fragments of similar sizes, usually several hundred base pairs. The sequencing efficiency can be improved if the DNA size selection is in the right range. Cat.# 20104S and 20104L are the best kits for NGS library size selection of illumina paired-end 100 (PE100) sequencing with 100-200 bp library inserts; Cat.# 20105S and 20105L are the best kits for NGS library size selection of illumina paired-end 150 (PE150) sequencing with 150-300 bp library inserts; and Cat.# 20106S and 20106L are the best kits for NGS library size selection of illumina paired-end 300 (PE300) sequencing with 300-600 bp library inserts.
Long-read sequencing uses a large DNA fragment as input and makes very long reads. Usually, library size selection is preferred to remove smaller fragments. Cat.# 20110S and 20110L are the best kits for long-read sequencing size selection with DNA sizes >5 kb, and Cat.# 20111S and 20111L are the best kits for long-read sequencing size selection with DNA sizes >10 kb.
The magnetic beads technology uses paramagnetic particles, also known as SPRI (Solid Phase Reversible Immobilization) beads, to bind DNA reversibly and selectively. DNA fragments can be size-selected and purified by changing the properties of the magnetic beads or SPRI beads. The magnetic beads can easily separate the beads-binding DNA from the contaminants and unwanted components in the samples. The samples after DNA size selection are free of contaminants such as buffer components, enzymes, proteins, salts, dNTPs, primers, and adapters. Our proprietary magnetic beads reagents improve yield, selectivity, and reproducibility.
Specific DNA fragments at a certain length range can be purified simply using magnetic separation with different beads components, avoiding tedious and time-consuming gel extraction and column-based purification. The magnetic beads method is popular for common DNA size selection, including library size selection. The first beads-binding step, referred to as the right-side clean-up, removes large DNA fragments. The large DNA fragments are bound to the beads and are discarded. The desired DNA fragments in the supernatant are transferred to a new well, and new beads are added to the supernatant for the second beads-binding, referred to as the left-side clean-up. The double-size selected DNA fragments are eluted after ethanol rinsing.
DNA size selection with dual clean-ups.
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A single clean-up is needed for DNA size selection with large fragments. In this case, only the large DNA fragments are bound to the beads. The selected larger DNA fragments are eluted after ethanol rinsing.
DNA size selection with single clean-up for >5 kb and >10 kb DNA.
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Features of DNA size selection and library size selection
High specificity and high recovery of size selection
11 selection ranges are available, including 5 ranges for NGS library size selection
50-100 bp
100-200 bp
200-500 bp
250-350 bp: ideal for illumina PE100 sequencing
300-450 bp: ideal for illumina PE150 sequencing
450-750 bp: ideal for illumina PE300 sequencing
500-1000 bp
1-3 kb
1-5 kb
>5 kb: ideal for long-read sequencing
>10 kb: ideal for long-read sequencing
Fast and simple
20-min protocol
No gel purification required
No columns required
No centrifugation required
Efficient removal of contaminants and unwanted components
