2-(Propargyl-PEG4-amido)-1,3bis(PEG1-methyl ester) is a crosslinker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. The methyl ester groups can be hydrolyzed, reduced, or substituted under different conditions.
Detail
2-(Propargyl-PEG4-amido)-1,3bis(PEG1-methyl ester) is a crosslinker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. The methyl ester groups can be hydrolyzed, reduced, or substituted under different conditions.
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resDNASEQ E.coli Residual DNA Quantitation kit (Without DNA Control)
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Product Info
Description
Features of the sDNASEQ E.coli Residual DNA
Quantitation kit include:
Simpler and Rapid
Only three steps will be need for Sample Preparation and All components of the Sample Preparation Kit can be stored at room temperature.
Only one Reagent for qPCR;
Only 1.5 hours will be needed for the whole test.
Accurate
Perfect amplification curve, good amplification efficiency and good precision.
Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology.
Limit of Detection (LOD): 1 pg/mL; Limit of Quantification (LOQ): 5 pg/mL
The recovery rate of different concentration samples in the linear range is between 70% and 130%
Kit Performance
Fig 1. Only three steps will be need for Sample Preparation and only 20 minitutes will be taken for Sample Preparation.
Fig 2. Seven concentration samples of 5fg/μL, 10fg/μL, 20fg/μL, 30fg/μL, 300fg/μL, 3pg/μL, 30pg/μL, 300pg/μL were detected. CV of each concentration was < 30%, Regression coefficient associated with standard solutions was 0.99975, and amplification efficiency was 100.068%.
Fig 3. Four concentration samples of 5fg/μL, 10fg/μL, 20fg/μL, and 30fg/μL were detected, and 10 multiple holes were detected for each concentration. The detection values of 5fg/μL and above were CV <30%.
Fig 4. DNA recovery can be determined by including samples spiked with known DNA amounts which are prepared from the corresponding DNA standards. Typically, the range for this value varies from 70% to 130%.
Fig 5. Only one Reagent for qPCR MIX.
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Note: Price not include shipment & duty, contact us to get full quote.
The resDNASEQ E.coli Residual DNA Quantitation kit is designed for the quantification of residual DNA from E. coli, in cell lines which are used for production of biopharmaceutical products. The resDNASEQ E.coli Residual DNA Quantitation kit use TaqManTM quantitative PCR to perform rapid, specifc quantitation of femtogram levels of residual host-cell or plasmid DNA. The kit was developed to meet the sensitivity requirements defined by WHO (10 ng E. coli DNA per therapeutic dose).
EXTRAClean Urine Exosome Purification and RNA Isolation Kits
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Ensure optimal results for sensitive applications like NGS
Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
Purification and enrichment of intact urinary exosomes for functional studies.
Bind and elute all RNA irrespective of size or GC content, without bias.
Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
No phenol extractions, Proteinase K treatment, nor carrier RNA required.
No time-consuming ultracentrifugation, filtration nor special syringes are required.
No precipitation reagents nor overnight incubation required.
Compatible with urine from any species.
Pure exosomes are purified and are free-from any other RNA-binding proteins.
Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary resin separation matrix.
Norgen’s EXTRAClean Urine Exosome Purification and RNA Isolation Mini Kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of RNA from different urine sample volumes ranging from 250 μL to 1 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The purified RNA is free from any protein-bound circulating RNA and of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, NGS application, Northern blotting, RNase protection and primer extension, and expression array assays.
All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
35-40 minutes
Average Yields
Variable depending on specimen
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Kits
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Overview
Columns bind proteins of interest while endotoxins flow through
Reduce endotoxin levels to less than 0.01 EU/µg of protein
Proteins are desalted
Greater than 95% protein recovery
Concentrate protein samples and remove detergents at the same time
Effectively remove a wide range of detergents including SDS, Triton® X-100, CHAPS, NP-40, and Tween 20
Purification is based on spin column chromatography that uses Norgen’s resin separation matrix
The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Kits are designed for the rapid removal of endotoxins from previously purified proteins or peptides, with protein recoveries of > 95% being achieved. Endotoxins, also known as lipopolysaccharides, are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins liberated by Gram-negative bacteria are frequent contaminations of protein solutions derived from bioprocesses. Due to the high toxicity of endotoxins in vivo and in vitro, their removal from protein preparations is often necessary prior to the use of the protein in downstream applications. These kits efficiently reduce endotoxin levels to ≤ 0.01 EU/mg of protein, using spin column chromatography based on Norgen’s proprietary resin as the separation matrix. These kits are highly efficient in removing many different salts commonly used in the laboratory including, but not limited to, MgCl2, NaCl, KCl, CaCl2, LiCl and CsCl. The purified protein samples can be used in a number of downstream applications including sequencing, cloning, and in vitro and in vivo introduction into cells and organisms for various purposes. The simultaneous removal of salts while concentrating a dilute protein solution makes the kit a convenient method for preparing proteins before running many downstream applications such as SDS-PAGE, isoelectric focusing, X-ray crystallography, NMR spectroscopy, mass spectroscopy and other applications.
Mini Kit
The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit is designed for the rapid removal of endotoxins from up to 200 μg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.
Maxi Kit
The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit is designed for the rapid removal of endotoxins from up to 4 mg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.
Including Triton® X-100, CHAPS, NP-40 and Tween 20
Minimum Elution Volume
50 μL
Time to Complete 10 Purifications
20 minutes
Storage Conditions All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.
Component
Cat. 22800 (25 preps)
Cat. 22200 (4 preps)
Binding Buffer J
8 mL
8 mL
Binding Buffer N
4 mL
20 mL
Wash Solution M
50 mL
130 mL
Wash Solution CIP
20 mL
60 mL
Wash Solution N
30 mL
130 mL
Wash Solution NIP
20 mL
60 mL
Elution Buffer G
6 mL
20 mL
Endotoxin Removal Solution
1.5 mL
1.5 mL
Protein Neutralizer EF
2 mL
2 mL
Maxi Spin Columns (assembled with Collection Tubes)
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4
Mini Spin Columns
25
–
Collection Tubes
25
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Maxi Spin Columns (assembled with Collection Tubes)