20 bp DNA Ladder on 3.6% of agarose gel, 8% and 12% of acrylamide gel.
• For sizing and quantification of double strand DNA fragments.
• Composed of eight bands as shown on right.
• Premixed with 6X DNA loading buffer for direct gel loading.
• For sizing and quantification of double strand DNA fragments.
• Composed of eight bands as shown on right.
• Premixed with 6X DNA loading buffer for direct gel loading.
20 bp DNA Ladder on 3.6% of agarose gel, 8% and 12% of acrylamide gel.
• For sizing and quantification of double strand DNA fragments.
• Composed of eight bands as shown on right.
• Premixed with 6X DNA loading buffer for direct gel loading.
The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Specifications
| Features | Specifications |
| Main Functions | Isolation miRNA from 0.3-0.5ml serum or plasma using magnetic particles |
| Applications | RT-PCR, cDNA synthesis, second generation sequencing |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
| Sample type | Serum, plasma, acellular samples |
| Sample amount | 300μl |
This product is based on the purification method of high binding magnetic particles. The sample material is denatured in Lysis Buffer. The protein is then precipitated by Protein Precipitation Solution and pelleted by centrifugation. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption.The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.
Advantages
Kit Contents
| Contents | R662801 | R662802 | R662803 |
| Purification Times | 48 Preps | 96 Preps | 5 x 96 Preps |
| MagPure Particles N | 1.7 ml | 3.5 ml | 17 ml |
| Buffer CFL | 6 ml | 12 ml | 60 ml |
| Buffer CPL | 1.8 ml | 3.5 ml | 20 ml |
| Buffer MGW1* | 30 ml | 60 ml | 250 ml |
| Buffer MW2* | 12 ml | 25 ml | 100 ml |
| RNase Free Water | 10 ml | 20 ml | 60 ml |
Storage and Stability
MagPure Particle N should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
This kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA (not include miRNA) from 20mg tissue, 150mg plant, 5 x 106 cell using two columns (gDNA removed column) |
| Applications | RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing |
| Purification method | Mini spin column |
| Purification technology | Silica technology, DNA filtration technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Animal soft tissue, cultured cells, lymphocytes, simple plant tissue |
| Sample amount | Cells:≤1 x 107 Animal tissue sample: 1-20 mgPlant tissue: 50-150 mg |
| Yield | 2-100μg |
| Elution volume | ≥50μl |
| Time per run | ≤25 minutes（1-24 samples） |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100µg |
The Kit isolates total RNA from up to 107 cells or 20 mg tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 25 mins. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to a RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit.
Advantages
Kit Contents
| Contents | R411102 | D411103 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure DNA Mini Columns | 50 | 250 |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml Collection Tubes | 100 | 2 x 250 |
| Buffer RLC | 50 ml | 200 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Buffer RW2* | 12 ml | 2 x 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Storage and Stability
HiPureKit can be stored dry at room temperature (15-25°C) and are stable for at least18 months under these conditions. During shipment, crystals or precipitationmay form in the Buffer RLC. Dissolve by warming buffer to 37°C.
This kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
These kits provide a convenient and rapid method for the isolation of microorganisms from water samples. The kits allows for the rapid isolation and purification of total RNA and DNA simultaneously from the microorganisms found in different types of water samples. The total RNA and DNA (including genomic DNA) are isolated from all the microorganisms found in the water, including bacteria, fungi and algae without the use of any inhibitory organic substances The kit purifies genomic DNA, and all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The purified RNA and DNA are highly concentrated, and can be used directly in a number of downstream applications including PCR, qPCR, RT-PCR, qRT-PCR, Northern blotting, Southern blotting and sequencing reactions.
Water RNA/DNA Purification Kit (Spin Column + Filters Kits)
These kits are available with 0.45 μm or 0.22 μm filter formats for capturing microorganisms present in the water.
Water RNA/DNA Purification Kit (Spin Column)
This kit is sold without filters columns.
Figure 1 / 2
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| Kit Specifications | |
| Minimum Water Input | 10 mL |
| Maximum Water Input | 100 mL |
| Maximum Filter Column Loading Volume | 20 mL |
| Maximum Spin Column Loading Volume | 650 µL |
| Elution Volume | 100 µL |
| Time to Complete 10 Purifications | 45 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.
| Component | Cat. 26400 (25 preps) | Cat. 26450 (25 preps) | Cat. 26480 (50 preps) |
|---|---|---|---|
| Lysis Buffer E | 15 mL | 15 mL | 2 x 15 mL |
| Wash Solution A | 18 mL | 18 mL | 38 mL |
| Enzyme Incubation Buffer B | 6 mL | 6 mL | 6 mL |
| Elution Buffer H | 6 mL | 6 mL | 6 mL |
| Mini Spin Columns | 25 | 25 | 50 |
| Filter Columns (0.22 μL) | 25 | – | – |
| Filter Columns (0.45 μL) | – | 25 | – |
| Bead Tubes | 25 | 25 | 50 |
| Collection Tubes | 25 | 25 | 50 |
| Elution Tubes (1.7 mL) | 25 | 25 | 50 |
| Product Insert | 1 | 1 | 1 |