A transparent, optically clear, DMSO resistant, non-tacky film which adheres only when pressure is applied. It is non-pierceable and peelable. Clear plastic, reflective, glossy on the top. Very thin and light and doesn’t crease easily.
Detail
Overview
A transparent, optically clear, DMSO resistant, non-tacky film which adheres only when pressure is applied. It is non-pierceable and peelable. Clear plastic, reflective, glossy on the top. Very thin and light and doesn’t crease easily.
Crystal clear seal specifically developed for optical applications, particularly qPCR
Non-sticky when removed from the packaging aiding with handling when using gloves
When the seal is in position, pressure can be applied to burst the capsules, releasing a strong adhesive only where the seal touches the raised well rims of the plate – the rest of the seal area above the wells remains optically clear
Other Products
Urine Exosome RNA Isolation Kit
Product Info
Document
Product Info
Overview
Isolation of exosomal RNA molecules from urine samples
Rapid and convenient spin-column protocol
Isolate inhibitor-free urinary microRNA for most downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Optional protocol for purification of exosomal proteins for western blot analysis
This kit provides a rapid spin column procedure for the isolation of exosomal RNA from urine samples. Users can simultaneously concentrate and isolate high quality exosomal RNA, including microRNA, for use in sensitive downstream assays such as RT-PCR, qRT-PCR, NGS, microarrays and more. The protocol can be completed in under 50 minutes. Urine volumes of 1 to 10 mL can be processed easily and rapidly. All sizes of RNA are recovered at an equal rate without the need for using hazardous chemicals like phenol.
Background
Exosomes are 40 – 150 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascite fluids, among other biological fluids. These vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. The exosomes contain cell-specific proteins, lipids and RNAs, which are transported to other cells, where they can alter function and/or physiology. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. Recent work has demonstrated the presence of distinct subsets of microRNAs within exosomes which can inform about the cell type from which the exosomes are secreted. For this reason, exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNases they can be efficiently recovered from biological fluids, such as urine.
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Permagen’s most universal ring magnet plate. From Low elution PCR applications up to 2 mL deep well, the X96 has you covered. No need to purchase two separate plates anymore. Smaller inner ring magnet allows for volumes as low as 5 µL, larger outer magnet handles up to 2 mL deep well
ANSI/ SBS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot on bottom, SBS/ SLAS fit on top to accept any microplate
Integrated Cushion base for maximum recovery. Helps aid in set-up, robot positioning inconsistencies, and labware consumable differences
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
Permagen’s most universal ring magnet plate. From Low elution PCR applications up to 2 mL deep well, the X96 has you covered. No need to purchase two separate plates anymore. Smaller inner ring magnet allows for volumes as low as 5 µL, larger outer magnet handles up to 2 mL deep well
This product is suitable for extracting total pathogen RNA from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified RNA can be used for clinical in vitro detection.
It is special designed for Pathogen RNA extraction from Sputum samples, can be used in tuberculosis (TB) detection.
Details
Specifications
Features
Specifications
Main Function
Extract total pathogen RNA from cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant, special design for sputum samples. Used in tuberculosis detection.
Applications
RT-PCR，PCR
Products
Pathogen RNA
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant, sputum
Sample amount
200-300μl
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
Advantages
Fast – several samples can be extracted in 40 minutes by column method
High quality – high purity total RNA / DNA can be directly used in various sensitive downstream applications
Safe – no phenol chloroform extraction required
Sensitive – DNA/RNA can be recovered at the level of PG
Kit Contents
Contents
IVD6672C
Purification Times
200 Preps
2ml Bead Tube
4 x 50
MagPure Particle
7.0 ml
Proteinase K
100 mg
Protease Dissolve Buffer
6 ml
DTT （Powder)
2g
Buffer SDS
15 ml
Buffer MLBN
220 ml
DNase I
4 x 0.6 ml
DNase Buffer
60 ml
Buffer MW1*
53 ml
Buffer MW2*
50 ml
Buffer NFW
30 ml
Storage and Stability
MagPure Particles and Proteinase K, DNase I should be stored at 2–8°C upon arrival. However, short-term storage (up to 2 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
Document
This product is suitable for extracting total pathogen RNA from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified RNA can be used for clinical in vitro detection.
