Permagen’s 24-Well magnetic separation plate was designed specifically for large volumes either manually or on a robotic platform. Dual ring magnet feature provide a ring of beads leaving the well bottoms clean for easy removal
Detail
Permagen’s 24-Well magnetic separation plate was designed specifically for large volumes either manually or on a robotic platform. Dual ring magnet feature provide a ring of beads leaving the well bottoms clean for easy removal
SBS SLAS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
The HiPure Plasmid EF Maxi Kit combines the power of HiPure technology with Magen’s innovative endotoxin removal technology (ETR) to deliver high-quality plasmid DNA with low endotoxin levels for use in eukaryotic transfection, and in vitro experiments. Up to 1000 µg high copy number plasmid DNA or 200 µg low copy number plasmid DNA can be purified from 150 mL overnight culture.
Isolation up to 1.5mg endotoxin-free plasmid DNA from 200ml bacterial culture
Applications
Cell transfection, animal injection, etc.
Purification method
Maxi spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Conventional plasmid vector
Sample amount
High copy plasmid vector: 100-150ml culture mediumLow copy plasmid vector: 150-200ml culture medium
Yield
200-1500μg
Elution volume
≥0.7ml
Time per run
≤60 minutes
Liquid carrying volume per column
20ml
Binding yield of column
1mg
Principle
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast – silica gel column purification is much faster than ion exchange
High yield – up to 1.5mg plasmid can be binded in one column
Ultra low endotoxin – content of endotoxin is less than 0.1 EU/μg, which can be directly used for cell transfection and animal injection, etc.
Kit Contents
Contents
P115602
P115603
Purification Times
10 Preps
50 Preps
RNase A
30 mg
150 mg
Buffer P1
100 ml
500 ml
Buffer P2
100 ml
500 ml
Buffer LN3
50 ml
250 ml
Buffer PW1
33 ml
180 ml
Buffer PW2
20 ml
100 ml
Elution Buffer
15 ml
120 ml
Buffer CL
33 ml
180 ml
HiPure DNA Maxi Columns C
10
50
Lysate Clear Midi Syringe
10
50
50 ml Collection Tubes C
20
100
Storage and Stability
The kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers,warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2–8°C.
The HiPure Plasmid EF Maxi Kit combines the power of HiPure technology with Magen’s innovative endotoxin removal technology (ETR) to deliver high-quality plasmid DNA with low endotoxin levels for use in eukaryotic transfection, and in vitro experiments. Up to 1000 µg high copy number plasmid DNA or 200 µg low copy number plasmid DNA can be purified from 150 mL overnight culture.
Peelable heat sealing film which is optically clear. Seal is suitable for qPCR and optical applications.
Heat sealing offers a 100% effective method for plate sealing for a complete seal integrity, as well as being quick and cost effective
Our ClearASeal Peel is an optically clear laminate film forming a peelable seal to polypropylene, polyethylene, polystyrene, polycarbonate and cyclic olefin copolymer (COC) plates
The optical clarity of this seal enables its use for sealing plates required for imaging use, including fluorescent detection methods such as qPCR and colorimetric assays
The ClearASeal Peel forms a complete seal to a plate enabling both low temperature uses, including low temperature storage, and high temperature uses, such as PCR (when used with a pressurized heated lid)
This seal demonstrates moderate solvent resistance and can be utilized for short term compound storage at room temperature
This seal is available as sheets, for use with manual and semi-automated sealers, such as our HeatASeal 500 Sealing Machine
Also available in multiple roll formats compatible with specified automated heat sealers, such as our Wasp or Chameleon XT
Our sheet seals are inter-leaved with paper sheets to help denote which side is the sealing side and aid removal of one sheet at a time from the pack
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Peelable heat sealing film which is optically clear. Seal is suitable for qPCR and optical applications.
Compact and stylish designed, the BioShake XP high-speed microplate mixer lets you perform all your standard mixing runs with a minimum of adjustments. The XP offers outstanding performance to handle a wide range of applications across biotechnology, pharmaceutical and academic research.
Small. Light. Efficient. Quiet.
Ideal for microplates & PCR plates
High-precision mixing from 200 up to 3,000 rpm
Universal spring clamps for easy fixation
Wide range of adapters
Stylish, fire resistant aluminium housing
ADVANCED TECHNOLOGY
Compact & stylish designed
Mixing tasks in microplates can be realized fast & safe with using adjustable speed of 200 up to 3,000 rpm.
The BioShake mixing-technology is obviously more robust, vibration free and needs less maintenance compared to classical mixers.
The first-class finished aluminium housing gives the BioShake XP its essential functionality. It provides a high amount of security, device stability and ensures a long service life.
GENTLE MIXING OF SAMPLES
By planar orbital motion
German designed and manufactured, BioShake XP plate mixer offer an ultra-efficient, 2-dimensional shaking axis so that samples mix completely in a fraction of the time of competing systems.
The mixing orbit of 2.0 mm is always constant. The orbital shaking is precisely controlled, in fact, that you need never spin down your plates after mixing. Even tubes, vials, high density plates, or low sample volumes, offer no obstacle for these precision tools.
Fully adjustable between 200 rpm and 3,000 rpm, well beyond the speeds of most other brands, guarantees fast, splatter-free, mixing for tubes, glass vials or across an entire 384-well microplate.
COMFORT
Simple intuitive operation and programming
The BioShake XP is operated from the front face of the enclosure which includes touch buttons and a display.
Special buttons for start, stop and storage of time and mixing modes enable the instrument to run complex applications. This opens new points of view on the daily laboratory work and optimizes routine application enormously.
The short mix button allows short and fast mixing in between.
The two line LCD display guarantees simultaneous and safe reading of all programmed and measured parameters as time and mixing frequency.
UNIVERSAL SPRING CLAMPS
Suitable for all microplates
In the corners eight spring clamps are inserted for easy fixation of skirted microplates anddeep well plates in SBS format 127.7 x 85.5 mm without an attached adapter. These spring clamps lock the microplate quickly and safely, even at the highest mixing frequencies.
For flexible and deformable PCR plates we recommend using of the related PCR adapter.
Fast mixing processes with frequent plate changes can be performed in the routine as easy and safe.
UNIQUE DESIGN MEETS HIGH-END TECHNOLOGY
First-class finished aluminium housing
Unique and efficient design combined with the most compact housing result in beautiful and well-defined laboratory equipment. Therefore, the first-class finished aluminium housing gives the BioShake XP its essential functionality. It provides a high amount of security, device stability and ensures a long service life.
Safety of the user and ease of use are clearly a priority for us.
EXCHANGABLE ADAPTERS
For a wide range of applications
The BioShake XP comes with a variety of standardized and specific adapter plates. The replacement of the adapters are very simple.
Perfect shaped adapters allow an optimal fit for standard tubes, lysis tubes, microplates, glass vials and other sample vessels.
MADE IN GERMANY
High-end instruments for daily routine
A perfectly harmonious blend of high-tech and handmade. “Made in Germany” has always been a recipe for success for QInstruments. 100% of QInstruments production takes place in Germany.
The focus is on human diligence and on being environmentally friendly. The company upholds its quality guarantee by means of consistent quality management. For more than 15 years these lab automation professionals from Jena have used only high-quality materials to ensure sustainable production, applied innovative thinking and undertaken research in a future-oriented way – all as a matter of course.
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Ideal for microplates & PCR plates High-precision mixing from 200 up to 3,000 rpm Universal spring clamps for easy fixation Wide range of adapters Stylish, fire resistant aluminium housing
