The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.
Specifications
Features | Specifications |
Main Functions | Isolation total RNA and miRNA from cell and tissue without MagZol reagent |
Applications | RT-PCR, Northern Blot, poly A+purification, nucleic acid protection and in vitro translation |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Cell, Animal tissue, Plant tissue |
Sample amount | Cells: ≤ 5 x 10^6, Animal tissue:<10mg |
Elution volume | ≥30μl |
Time per run | ≤40 minutes |
Liquid carrying volume per column | 800µl |
Binding yield of column | 100µg |
Biological samples are first lysed and homogenized in a highly denaturing guanidine isothiocyanate-containing buffer, which immediately inactivates DNases and RNases to ensure isolation of intact DNA and RNA. The lysate is then passed through a Mini spin column. This column, in combination with the high-salt buffer, allows selective and efficient binding of genomic DNA. Flow-through from the column is digested by Proteinase K in the presence of ethanol. This optimized digestion, together with the subsequent addition of further ethanol, allows appropriate binding of total RNA, including miRNA, to the column. Contaminants are efficiently washed away and high-quality RNA is eluted.
Advantages
Kit Contents
Contents | R431102 | R431103 |
Purification Times | 50 Preps | 250 Preps |
HiPure RNA Mini Columns | 100 | 2 x 250 |
2ml Collection Tubes | 100 | 2 x 250 |
Proteinase K | 48 mg | 240 mg |
Protease Dissolve Buffer | 5 ml | 15 ml |
Buffer RLC | 40 ml | 200 ml |
Buffer RWC | 20 ml | 80 ml |
Buffer RW2* | 20 ml | 2 x 50 ml |
RNase Free Water | 10 ml | 60 ml |
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.
029172 Oxidase Test Reagent
Usage: For Oxidase Test.
Specification:10pcs/box
10pcs/box
Usages:
For cultivation of Escherichia coli.
Principle:
Peptone provide nitrogen, vitamins and growth factors; sodium chloride to maintain osmotic equilibrium; lactose into fermentable sugars.
Formulation(per liter):
Pancreatic Digest of Gelatin | 20 g |
Lactose Monohydrate | 10g |
Dehydrated Ox Bile | 5g |
Bromocresol Purple | 10mg |
final pH | 7.3±0.2 |
How to use:
1.Suspend 35g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 ℃ autoclave for 15min.
2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 250g/bottle
250g
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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