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24×15ml Angle Rotor 

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Other Products

DBCO-TFP ester

DBCO-TFP ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The highly reactive DBCO group can be used in copper-free click reactions. The TFP ester can react with primary amine groups and is also less susceptible to undergo hydrolysis compared to NHS ester. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Sulfo DBCO-PEG4-amine

Sulfo DBCO-PEG4-Amine is a water-soluble carboxyl-reactive building block with extended PEG spacer arm. In the presence of of activators (e.g. EDC, or HATU), this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. The hydrophilic sulfonated spacer arm greatly improves water solubility of DBCO derivatized molecules and a provides a long and flexible connection. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

cf-DNA/cf-RNA Preservative Tubes

Overview

  • Preservation and isolation of both cf-DNA and cf-RNA from a single tube
  • Fixative-free preservative, no cross-linking of DNA
  • Preserve cf-DNA/ct-DNA for 30 days at ambient temperature and for up to 8 days at 37°C
  • Preserve cf-RNA for 30 days at ambient temperature
  • Preserve Circulating Tumour Cells (CTCs) for 14 days at ambient temperature
  • No plasma volume loss after shipping/transportation
  • Prevent hemolysis allowing better separation of plasma
  • Prevent apoptosis of blood cells and fragmentation of genomic DNA
  • Produce high quality/quantity of plasma cf-DNA/ct-DNA/cf-RNA
  • Vacuumed to draw 8.7 mL of blood in 10 mL tubes
  • The procedure for plasma isolation using this kit is compliant with ISO 20186-3:2019

Norgen’s cf-DNA/cf-RNA Preservative Tubes are closed, evacuated plastic tubes for the collection and the preservation of cf-DNA, circulating tumor DNA, cf-RNA and circulating tumor cells in human whole blood samples during storage and shipping. Using these tubes in combination with Norgen’s Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen’s Plasma/Serum RNA Purification Kits enables the purification and concentration of an inhibitor-free cf-DNA/ct-DNA/cf-RNA in a very small elution volume. Plasma recovered from Norgen’s cf-DNA/cf-RNA Preservative Tubes is also compatible with any other cf-DNA and/or cf-RNA purification method. The purified cf-DNA, ct-DNA and cf-RNA are compatible with any downstream application assays, including PCR, qPCR, rt-qPCR, methylation-sensitive PCR, Southern Blot analysis, gene expression analysis, microarrays and NGS.

This product is for research use only and not for use in diagnostic procedures.

Performance

Norgen’s cf-DNA/cf-RNA Preservative Tubes abridge the collection/processing of whole blood for the subsequent purification of cf-DNA and/or cf-RNA. The cf-DNA/cf-RNA Preservative Tubes preserve and inhibit the programmed cell-death (apoptosis) of the blood, hence preventing the release of intracellular DNA/RNA into plasma. Whole blood samples can be shipped and stored at room temperature for the subsequent processing of plasma. The cf/ct DNA and cf-RNA levels are stable for up to 30 days at room temperature. The cf-DNA is also stable for up to 8 days at 37°C. When blood collected on Norgen’s cf-DNA/cf-RNA tubes is processed for plasma recovery no buffy coat will be generated and the Circulating tumor cells (CTCs) will be located at the bottom of the tube with the blood sediment. CTCs are stable for 14 days at room temperature where it can be extracted from the blood sediment or from whole preserved blood for cell sorting or for cf/DNA or cf-RNA purification.

Plasma samples produced from the whole blood sample collected into Norgen’s cf-DNA/cf-RNA Preservative Tubes and purified using Norgen’s Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen’s Plasma/Serum RNA Purification Kits yield concentrated, high-quality and inhibitor-free cf-DNA/ct-DNA or cf-RNA for any downstream application.

Workflow

workflow

NOTE: This product is for research use only. For the CE marked version, see Cat. Dx63950

Details

Supporting Data

Figure 1 / 8

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Figure 1. Effect of ambient temperature storage
Figure 1. Effect of ambient temperature storage
Figure 2. No plasma volume loss after shipping/transportation
Figure 3. Hemolysis of collected blood measured over time
Figure 4. Prevent cell lysis and the release of gDNA and accumulation of apoptotic ladder in plasma
Figure 5. Effect of high temperature (37 C) storage for 8 days
Figure 6. High Quantity of cf-DNA from Plasma preserved
Figure 7. Effect of ambient temperature storage on cf-RNA
Figure 8. Effect of ambient temperature storage

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Kit Specifications
Tube Size16 x 100 mm
Tube TypePET Tube
Blood Draw Volume8.7 mL in 10 mL Tubes
Preservative Volume1.3 mL
Recovered Plasma VolumeApproximately 6-7 mL
(not affected by shipping)
AnticoagulantProprietary
PreservativeNon formaldehyde – Proprietary preservation agent
Length of cf-DNA PreservationUp to 30 days at room temperature (15-25°C)
Up to 8 days at 37°C
Length of cf-RNA PreservationUp to 30 days at room temperature (15-25°C)
Length of CTCs PreservationUp to 14 days at room temperature (15-25°C)


Storage Conditions and Product Stability

  1. When stored at 15-30°C, unfilled Norgen cf-DNA/cf-RNA Preservative Tubes are stable through the expiration date.  See tube label for expiry date.
  2. Short-term storage/shipping at -20°C to 37°C for up to 10 days is acceptable for unfilled Norgen cf-DNA/cf-RNA Preservative Tubes.
  3. Do not freeze unfilled Norgen cf-DNA/cf-RNA Preservative Tubes for long term storage over 10 days. Freezing unfilled tubes may lead to loss of vacuum and precipitation may occur.
  4. Cell-free DNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes is stable for up to 30 days when stored at room temperature (15-25°C)
  5. Cell-free DNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes is stable for up to 8 days when stored at 37ºC.
  6. Cell-free RNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes is stable for up to 30 days when stored at room temperature (15-25°C)
  7. Circulating tumor cells (CTCs) in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes are stable for up to 14 days at room temperature (15-25ºC).