24×15ml Angle Rotor 

Overview

• Convenient
• One cDNA Synthesis, Multiple microRNAs and microRNA-targets analyzed
• Time Savings
• Cost Efficient
• High Sensitivity and Yield
• Robust Enzyme
• Available in 12 or 50 reaction size

Norgen’s microScript microRNA cDNA Synthesis Kit is an all-in-one, ready-to-use product for the reverse transcription of microRNA from either Total RNA preparations or enriched microRNA preparations. The kit contains the 2x Reaction Mix and the microScript microRNA Enzyme Mix. The kit utilizes Norgen’s microScript Reverse Transcriptase, a mutant version of Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase. It has reduced RNase H activity and increased thermal stability.

The workflow of Norgen’s microScript microRNA cDNA Synthesis Kit involves a simple, single-tube set-up by the mixing of 2x Reaction Mix, Enzyme Mix and the RNA template. The reaction can then be carried out in a thermocycler. A poly (A) tail is first added to the RNA template, followed by cDNA synthesis using an adapter primer. In addition to the ease-of-use, the single-tube set-up provides superb consistency and sensitivity. The cDNA could be used in a PCR or qPCR amplification using a Universal PCR Reverse Primer and the forward primer that contains the sequence of the microRNA of interest. A single cDNA preparation could be used for PCR amplification of a number of different microRNAs. In addition, the cDNA preparation could be used for PCR or qPCR detection (using gene-specific forward and reverse primers) of mRNA or large RNA if total RNA preparation was the starting template. This could allow for parallel evaluation of expression level of microRNAs and microRNA-targets.

Details

Supporting Data

Figure 1 / 2

Previous

Next

Click for expanded view

Storage Conditions and Product Stability
This kit is stable for 1 year after the date of shipment .

Component	Cat. 54415 (12 rxns)	Cat. 54410 (50 rxns)
microScript microRNA Enzyme Mix	12 μL	50 μL
2x Reaction Mix	120 μL	500 μL
Universal PCR Reverse Primer	60 μL	250 μL
Nuclease-Free Water	1.25 mL	2 x 1.25 mL
Product Insert	1	1

endo-BCN-PEG2-alcohol is a click chemistry PEG linker. The BCN group is very reactive with azide-tagged molecules. The hydroxyl group enables further derivatization or replacement with other reactive functional groups. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

endo-BCN-PEG2-alcohol is a click chemistry PEG linker. The BCN group is very reactive with azide-tagged molecules. The hydroxyl group enables further derivatization or replacement with other reactive functional groups. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

The Permagen 96-well 0.2 mL PCR Strip Rack was designed for use in manual or automation applications where strip tubes are required. It can also be used with our MSP750 MSP750R MSPU650R or MSPU650 magnet plates and is available as a discounted kit   Picture shown is SKU: MSPU650RKT for reference 

SKU #

MTP96

Features

• SBS/ SLAS footprint
• Laser labeled rows and columns for easy sample control
• Precision manufactured for more consistent results
• Works with strips and individual tubes
• Made in USA 
• For Research use only

Compatibility

• 8-strip 0.2 mL PCR Strips 
• 12-strip 0.2 mL PCR Strips 
• Individual PCR tubes
• PCR Plates (Unskirted-skirt)

The Permagen 96-well 0.2 mL PCR Strip Rack was designed for use in manual or automation applications where strip tubes are required. It can also be used with