24×50ml Angle rotor 

• Twenty 4.5X60mm strips of each combination of nitrocellulose (4X), wicking material (2X), and sample pad (3X)
• A total of 480 strips are provided
• 100 cassettes
• 125mL of buffer

Description

Blank lateral flow strips are the perfect companion during the development and feasibility testing of a lateral flow device.  IN THE BEGINNING, the capture and test line reagents can be spotted onto the nitrocellulose portion of the blank strips at different concentrations, volumes and buffer conditions.  This enables the user to determine the optimal conditions for subsequent spraying and enables the user to verify the test and controls will respond according to expectations before scaling up the process.

These blank strips are compatible with all of our colloidal gold products AND:

Lateral flow cassettes: C02022

Streptavidin CdSe/ZnS Quantum Dots For Lateral Flow: AU2043

Streptavidin Europium Chelate Microspheres For Lateral Flow: AU2050

Streptavidin Colloidal Gold For Lateral Flow: AU2017

RNase-Free Lateral Flow Assay Running Buffer Screening Pack: AU2035

Twenty 4.5X60mm strips of each combination of nitrocellulose (4X), wicking material (2X), and sample pad (3X)
A total of 480 strips are provided
100 cassettes
125mL of buffer

DBCO-C5-PEG12-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. DBCO group is a highly reactive cycloalkyne which is commonly used for copper-free Click Chemistry reactions. The hydrophilic PEG chain allows for increased water solubility of compounds in aqueous media. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. HATU) to form a stable amide bond. Reagent grade, for research use only.

DBCO-C5-PEG12-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. DBCO group is a highly reactive cycloalkyne which is commonly used for copper-free Click Chemistry reactions. The hydrophilic PEG chain allows for increased water solubility of compounds in aqueous media. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. HATU) to form a stable amide bond. Reagent grade, for research use only.

Overview

• Fast and easy processing using a rapid spin-column format
• Isolate high quality genomic DNA from fresh or preserved saliva samples
• Compatible with preserved saliva samples collected using Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000)

This kit provides a fast and simple spin column procedure for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000), as well as fresh saliva samples.

Saliva DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream research applications including PCR, Southern Blot analysis, sequencing and microarray analysis.

Background

Saliva represents an excellent non-invasive alternative to blood collection. Human genomic DNA extracted from buccal epithelial cells and white blood cells found in saliva can be used in various applications in diagnostics. Saliva DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Saliva DNA can also be used to diagnose particular types of infections. Isolation of DNA from saliva has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive, the samples can be collected by individuals with little training, and no special equipment is required. Norgen’s Saliva DNA Isolation Kit provides a fast and simple procedure for isolating genomic DNA from both preserved saliva samples and fresh saliva samples.

Details

Supporting Data

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Kit Specifications
Maximum Saliva Input	0.5 mL preserved saliva 0.25 mL fresh saliva
Average Yield from 0.25 mL of Saliva*	3 – 7 μg
Average Purity (OD260/280)	1.7 – 2.1
Time to Complete 10 Purifications	30 minutes

* Average yield will depending on the donor

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Component	Cat. RU45400 (50 preps)
Lysis Buffer F	30 mL
Proteinase K in Storage Buffer	1.2 mL
Binding Buffer B	12 mL
Wash Solution A	18 mL
Elution Buffer B	15 mL
Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1