25 Tube Lab Freezer Box with attached Lid (pack of 10)

Overview

• Isolate genomic DNA from all types of bacteria (both Gram-positive and Gram-negative)
• Rapid and convenient spin column protocol
• Purified bacterial gDNA has a minimal host gDNA contamination.
• High yield, high quality DNA for sensitive downstream applications including sequencing, PCR, qPCR and more

Synovial fluid is secreted into the joint cavity from the inner membrane of synovial joints. Synovial fluid is a plasma ultrafiltrate which contains proteins derived from both the blood plasma and produced by cells within the joint tissues. It lubricates the articulating joints as well as supplies oxygen and nutrients while removing carbon dioxide and metabolic wastes from the chondrocytes in the surrounding cartilage. Septic arthritis is usually caused by bacterial, viral or fungal infections to the synovial fluid. Staphylococcus aureus is the most common bacterial infection causing Septic arthritis. Diagnosing Septic arthritis is done through joint fluid (synovial fluid) analysis, blood tests or imaging tests.  

Norgen’s Synovial Fluid Bacterial Genomic DNA Isolation Kit provides a fast, reliable and simple procedure for isolating the highest quality and the highest quantity of bacterial genomic DNA from various amounts of synovial fluid ranging from 1 mL up to 10 mL.  Purification is based on using Norgen’s proprietary resin separation matrix. The kit is designed to isolate all Gram +ve and Gram -ve strains. Moreover, the kit allows the user to elute the purified bacterial genomic DNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified bacterial gDNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR, Southern Blot analysis and NGS.

Details

Supporting Data

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Kit Specifications
Maximum Input	2 x 109 bacterial cells
Column Binding Capacity	25 μg
Average Yield	up to 20 µg
Time to Complete 10 Purifications	45 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Component	Cat. 67900 (50 preps)
Precipitation Solution B	15 mL
Lysis Buffer T	6.5 mL
Solution BX	28 mL
Wash Solution A	38 mL
Elution Buffer B	8 mL
Micro Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

K-TDFC

SKU: 700004345

Contains 6 Controls: Use with K-TDFR, K-INTDF or K-RINTDF

Content:	Contains 6 Controls: Use with K-TDFR, K-INTDF or K-RINTDF
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: Ambient, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:

Dietary Fiber

For use with the Total Dietary Fiber Assay Kit. Contains barley β-glucan, high amylose maize starch, wheat starch, casein, pectin and larch galactan. Wheat arabinoxylan is available on request.

See more of our starch and dietary fiber products. 

For use with the Total Dietary Fiber Assay Kit. Contains barley β-glucan, high amylose maize starch, wheat starch, casein, pectin and larch galactan. Wheat arabinoxylan is available on request.