25 Tube Lab Freezer Box with attached Lid (pack of 10)

【Material】The vial storage box is made of durable cardboard with waterproof coating and cardboard dividers；chemically resistant to alcohols and mild organic
【Temperature Range】These freezer boxes are stable from -196°C to 121°C
【Applications】The freezer box is suitable for iquid nitrogen freezing (vapor phase)
【Capacity】Size: Height of 2 inch, Holds 0.5ml, 1.5ml, 2.0ml tubes
【Excellent Cusomer Service】We are dedicated in providing the best products and services to customers. If you have any problem, please feel free to contact us. We will help you solve the problem as soon as possible.

Detail

Description

【Material】The vial storage box is made of durable cardboard with waterproof coating and cardboard dividers；chemically resistant to alcohols and mild organic
【Temperature Range】These freezer boxes are stable from -196°C to 121°C
【Applications】The freezer box is suitable for iquid nitrogen freezing (vapor phase)
【Capacity】Size: Height of 2 inch, Holds 0.5ml, 1.5ml, 2.0ml tubes
【Excellent Cusomer Service】We are dedicated in providing the best products and services to customers. If you have any problem, please feel free to contact us. We will help you solve the problem as soon as possible.

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Water RNA/DNA Purification Kits

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Overview

Isolate total DNA and RNA from all microorganisms found in water, including bacteria, fungi and algae
RNA and DNA are both column purified simultaneously using the same column
Elution contains concentrated DNA and RNA without the need for further precipitation
Complete RNA (including microRNA) without phenol
Isolated RNA and DNA are of high quality and integrity for all downstream applications
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

These kits provide a convenient and rapid method for the isolation of microorganisms from water samples. The kits allows for the rapid isolation and purification of total RNA and DNA simultaneously from the microorganisms found in different types of water samples. The total RNA and DNA (including genomic DNA) are isolated from all the microorganisms found in the water, including bacteria, fungi and algae without the use of any inhibitory organic substances The kit purifies genomic DNA, and all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The purified RNA and DNA are highly concentrated, and can be used directly in a number of downstream applications including PCR, qPCR, RT-PCR, qRT-PCR, Northern blotting, Southern blotting and sequencing reactions.

Water RNA/DNA Purification Kit (Spin Column + Filters Kits)

These kits are available with 0.45 μm or 0.22 μm filter formats for capturing microorganisms present in the water.

Water RNA/DNA Purification Kit (Spin Column)

This kit is sold without filters columns.

Details

Supporting Data

Figure 1 / 2

Figure 1. High Yield and Purity of RNA and DNA

Click for expanded view

Kit Specifications
Minimum Water Input	10 mL
Maximum Water Input	100 mL
Maximum Filter Column Loading Volume	20 mL
Maximum Spin Column Loading Volume	650 µL
Elution Volume	100 µL
Time to Complete 10 Purifications	45 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.

Component	Cat. 26400 (25 preps)	Cat. 26450 (25 preps)	Cat. 26480 (50 preps)
Lysis Buffer E	15 mL	15 mL	2 x 15 mL
Wash Solution A	18 mL	18 mL	38 mL
Enzyme Incubation Buffer B	6 mL	6 mL	6 mL
Elution Buffer H	6 mL	6 mL	6 mL
Mini Spin Columns	25	25	50
Filter Columns (0.22 μL)	25	–	–
Filter Columns (0.45 μL)	–	25	–
Bead Tubes	25	25	50
Collection Tubes	25	25	50
Elution Tubes (1.7 mL)	25	25	50
Product Insert	1	1	1

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Blood DNA Isolation Kits

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Overview

High yield and high purity DNA ready for any application
Available in a variety of formats to properly suit your needs
Compatible with blood collected on a variety of commercially available tubes

These kits allow for the isolation of DNA from the blood of various species, including humans and will recover genomic DNA, mitochondrial DNA, viral DNA and bacterial DNA. The purified DNA is of excellent quality and yield and completely compatible with any downstream application including PCR, qPCR and genotyping.

Blood DNA Isolation Mini Kit

Norgen’s Blood DNA Isolation Mini Kit is designed for the rapid preparation of DNA from up to 200 µL of whole blood using a rapid spin column protocol. Preparation time for a single sample is less than 30 minutes and each kit contains sufficient materials for 50 preparations.

Blood DNA Isolation Midi Kit

Norgen’s Blood DNA Isolation Kit is designed for the rapid spin column preparation of DNA from 0.3 to 2 mL volumes of whole blood. Preparation time for a single sample is less than 30 minutes, and each kit contains sufficient materials for 20 preparations.

Blood DNA Isolation Maxi Kit

This kit is designed for the rapid preparation of DNA from 3 mL up to 10 mL of whole blood. Preparation time for a single sample is less than 30 minutes.

Blood DNA Isolation 96-Well Kit (High Throughput)

This kit provides a rapid method for the high-throughput isolation of DNA from up to 200 µL of whole blood. Fast and easy processing using either a vacuum manifold or centrifugation.

Blood DNA Isolation Kit (Magnetic Bead System)

Norgen’s Blood DNA Isolation Kit (Magnetic Bead System) is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood from various species, including human. Purification is based on magnetic beads as the separation matrix. Norgen’s magnetic beads bind DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The genomic DNA is preferentially purified from other cellular proteinaceous components. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including real-time PCR, NGS and microarray analysis.

Blood DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)

96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Details

Supporting Data

Figure 1 / 19

Figure 1. The difference of Ct values obtained from a Taqman qPCR reaction performed on DNA isolated from blood collected on Citrate, EDTA, and Heparin anticoagulants

Figure 2. Resolution of DNA isolated from buffy coats using Norgen's Blood Genomic DNA Isolation Mini Kit

Figure 3. The difference in Ct values between DNA isolated using Norgen's Blood Genomic DNA Isolation Mini Kit (Norgen), Competitor A's DNA Blood Mini Kit (A), Competitor B's Blood Kit (B), and Competitor B's Dx Blood (B Dx), in a Taqman qPCR reaction

Figure 4. The difference in yield isolated from different volumes of blood using Norgen's vs Qiagen's kit.

Figure 7. High Yields of DNA Isolated from 20 µL to 200 µL of Whole Blood

Figure 8. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction

Figure 9. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction

Figure 10. High Yields of DNA Isolated from 2 mL of Whole Blood

Figure 11. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction

Figure 12. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction

Figure 13. High Yields of DNA Isolated from 200 µL of Whole Blood

Figure 14. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction

Figure 15. DNA Isolated from Blood Preserved in 3 Different Anticoagulants

Figure 17. Detection of GAPDH from blood samples preserved in three different anticoagulants (Heparin: red, EDTA: green and Na Citrate: blue)

Figure 18. DNA Isolated from Blood Preserved in EDTA Anticoagulants

Figure 19. Detection of GAPDH From Blood Samples Preserved in EDTA Anticoagulants

Click for expanded view

Kit Specifications
Maximum Blood Input	200 μL
Column Binding Capacity	> 50 μL
Average Yield (200 μL of blood)	2-8 μg*
Time to Complete 96 Purifications	45 minutes

*Yield will vary depending on the type of blood processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Component	Cat. 46300 (50 preps)	Cat. 46380 (100 preps)	Cat. 51400 (20 preps)	Cat. 31200 (12 preps)	Cat. 46350 (192 preps)	Cat. 59800 (50 preps)	Cat. 62600 (192 preps)
Lysis Buffer B	20 mL	2 x 20 mL	2 x 40 mL	2 x 110 mL	2 x 40 mL	20 mL	1 x 40 mL 1 x 20 mL
Solution WN	18 mL	2 x 18 mL	55 mL	55 mL	55 mL	18 mL	55 mL
Wash Solution A	18 mL	2 x 18 mL	2 x 38 mL	2 x 38 mL	2 x 38 mL	–	–
Elution Buffer B	30 mL	2 x 30 mL	30 mL	30 mL	2 x 30 mL	15 mL	1 x 30 mL 1 x 15 mL
Proteinase K in Storage Buffer	1.2 mL	2 x 1.2 mL	4.4 mL	8 mL	4.5 mL	1.2 mL	4 mL
Magnetic Bead Suspension	–	–	–	–	–	2 x 1.1 mL	8.5 mL
Maxi Spin Columns	–	–	–	12	–	–	–
Mini Spin Columns	50	100	–	–	–	–	–
Midi Spin Columns	–	–	20	–	–	–	–
96-Well Plate	–	–	–	–	2	–	2
Adhesive Tape	–	–	–	–	4	–	2
Collection Tubes	50	100	20	12	–	–	–
96-Well Collection Plate	–	–	–	–	2	–	–
Elution Tubes	50	100	20	12	–	50	–
96-Well Elution Plate	–	–	–	–	2	–	2
Product Insert	1	1	1	1	1	1	1

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25 Tube Lab Freezer Box with attached Lid (pack of 10)

Detail

Description

Other Products

Amine-Tri(3-methoxypropanamide-PEG10-Propargyl) Methane HCl salt

Water RNA/DNA Purification Kits

Overview

Details

Supporting Data

Blood DNA Isolation Kits

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Details

Supporting Data

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