1 Layer Cell Factory One Wide Mouth And One Narrow Mouth

Huayi Cell Factory is a robust and easy-to-use cell culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.

To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.

Specifications: 1 layer, 2 layers, 5 layers, 10 layers and 40 layers.

PRODUCT FEATURES

• The product is made of medical grade USP CLASS VI polymer polystyrene
• The product is made under a 100,00- class dust-free manufacturing site
• Surface TC treatment ensures efficient cell attachment
• The product assembled with ultrasonic welded technology
• Versatile port design facilitates both pouring and aseptic filling techniques
• Gamma radiation sterilization
• the cell culture surface area

of one 10-layer Cell Factory unit is equivalent to the area

of 36 T-175 flasks

Huayi Cell Factory is a robust and easy-to-use cell culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.

To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.

Specifications: 1 layer, 2 layers, 5 layers, 10 layers and 40 layers.

Introduction

HiPure DNA Clean Up 96 Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

Details

Specifications

Features	Specifications
Main Functions	Recover DNA fragments between 60bp-10kbp from PCR, Reactions, crude DNA using 96 well Bind Plate
Applications	PCR, sequencing, labeling reactions, ligations and restriction digestion, etc.
Purification method	96 well Bind Plate
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	PCR product, enzymatic reaction
Sample amount	Appropriate
Recovery	≥80%
Elution volume	≥75μl
Time per run	≤60 minutes
Liquid carrying volume per column	800µl
Binding yield of column	20µg

Principle

The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Advantages

• High recovery efficiency – ≥80% DNA recovery
• Fast – isolation can be completed in 60 minutes

Kit Contents

Contents	D212201	D212202	D212203
Purification Times	1 x 96 Preps	4 x 96 Preps	20 x 96 Preps
Buffer GDP	120 ml	400 ml	4 x 400 ml
Buffer DW2	20 ml	100 ml	3 x 100 ml
Elution Buffer	20 ml	20 ml	30 ml
HiPure DNA Plate	1	4	20
2.2ml Collection Plate	1	4	20
1.6 ml Collection Plate	1	4	20
0.8 ml Collection Plate	1	4	20

Storage and Stability

The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.

HiPure DNA Clean Up 96 Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.