Name of Product
Milenia GenLine Extraction System
Catalog Number
MGES 1
Short Info
The Milenia GenLine Extraction System is an universal kit which is intended for the isolation of analytes from swabs for further processing.
Method/Platform
Swab Sample Collection System
Range/Assay Sensivity
Test Principle
Brief Instructions
Storage
room temperature
Components
1000 pcs. of reaction tubes (1,5 ml) and 1000 pcs. of mini purification column
Name of Product
Milenia GenLine Extraction System
Catalog Number
MGES 1
Short Info
The Milenia GenLine Extraction System is an universal kit which is intended for the isolation of analytes from swabs for further processing.
Method/Platform
Swab Sample Collection System
Range/Assay Sensivity
Test Principle
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Specifications
Features | Specifications |
Main Functions | Extract viral DNA/RNA from 200μl cell-free samples by magnetic beads |
Applications | RT-PCR,PCR,NGS |
Products | Viral DNA / RNA |
Purification method | Polydisperse magnetic beads |
Purification technology | Magnetic beads technology |
Process method | Manual or automatic |
Sample type | cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant |
Sample amount | 200μl |
Adaptive instrument | Nucleic acid extractor, pipetting workstation |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Nuclease Free Water.
Advantages
Kit Contents
Contents | IVD5412 |
Purification Times | 200 Preps |
MagPure Particles N | 5 ml |
PK/Carrier RNA | 50 mg |
Protease Dissolve Buffer Blue | 5 ml |
Buffer MLB* | 120 ml |
Buffer MW1* | 53 ml |
RNase Free Water | 30 ml |
Storage and Stability
This kit is shipped and stored at room temperature and is valid for 12 months.
Experiment Data
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Product Description
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).
Technical Parameters:
Parameters | Details |
---|---|
Product Name | DNA Isothermal Amplification Kit NFO |
Manufacturer | Amp-future |
Storage Temperature | -20°C |
Kit Components | Enzymes, Buffers ,Reagents |
Packaging | 48 Tests/box |
Detection Limit | 500-1000copies/µL |
Shipping | ICE |
Test Time | 5-20mins |
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Isothermal nucleic acid Applications
Suitable for DNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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