The HiPure Minipreps system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Miniprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Specifications
Features | Specifications |
Main Functions | Isolation up to 30μg endotoxin-free plasmid DNA from 1-5ml bacterial culture using 96-well bind plate |
Applications | Cell transfection, animal injection, etc. |
Purification method | 96 well plate |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Low copy plasmid vector |
Sample amount | 1-5ml LB(x96) |
Yield | 30μg |
Elution volume | ≥75μl |
Time per run | ≤60 minutes |
Liquid carrying volume per column | 800μl |
Binding yield of column | 70μg |
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method,then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
1. High throughput: processing 96 samples at once;
2. High yield: The purified plasmid yield can reach up to 30μg, which is greater than the yield of the magnetic bead method kit;
3. Detoxification: endotoxin content <0.1EU/μg;
4. Fast: The entire extraction can be completed in 60 minutes without the need for time-consuming alcohol precipitation;
Kit Contents
Contents | P115701 | P115702 | P115703 |
Purification Times | 1 x 96 Preps | 4 x 96 Preps | 20 x 96 Preps |
RNase A | 10 mg | 20 mg | 100 mg |
Buffer P1 | 30 ml | 120 ml | 550 ml |
Buffer P2 | 30 ml | 120 ml | 550 ml |
Buffer LEN3 | 15 ml | 60 ml | 300 ml |
Buffer LN4 | 80 ml | 270 ml | 2 x 700 ml |
Buffer LN5 | 40 ml | 220 ml | 1100 ml |
Buffer PW1 | 40 ml | 220 ml | 1100 ml |
Buffer PW2 | 25 ml | 100 ml | 2 x 200 ml |
Elution Buffer | 30 ml | 60 ml | 250 ml |
Lysate Clear Plate | 1 | 4 | 20 |
HiPure DNA Plate | 1 | 4 | 20 |
1.6ml Collection Plate | 2 | 8 | 40 |
0.5ml Collection Plate | 1 | 4 | 20 |
Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A,Buffer P1 is stable for 6 months when stored at 2-8°C.
For any technical problems or customized products, please contact us.
F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here
The HiPure Minipreps system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Miniprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
Name of Product
Echinococcus multilocularis (Em18) – IgG ELISA
Catalog Number
AF 9310
Short Info
This ELISA kit is intended for the quantitative detection of IgG antibodies against Em-18 antigen of Echinococcus multilocularis in human serum.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Method/Platform
ELISA in microplate format
Range/Assay Sensivity
pNPP, λ=405 nm
Test Principle
Specific antibodies in the sample bind to Echinococcus multilocularis Em18 antigen sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate.
12 x 8 strips (96 tests)
This ELISA kit is intended for the quantitative detection of IgG antibodies against Em-18 antigen of Echinococcus multilocularis in human serum.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
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