Propargyl-PEG10-NHS ester serves as a bifunctional PEG linker with a terminal propargyl that participates in click reactions with azide-bearing moieties and an NHS ester group that reacts readily and efficiently with amine-bearing molecules. The PEG10 units improve the water solubility of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG10-NHS ester serves as a bifunctional PEG linker with a terminal propargyl that participates in click reactions with azide-bearing moieties and an NHS ester group that reacts readily and efficiently with amine-bearing molecules. The PEG10 units improve the water solubility of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Control System:
5.4”LCD Touch Screen Display
>The display can simultaneously display:all main parameters such as cold trap temperature,vacuum degree,sample temperature, and shelf temperature.
>Schematic diagram of equipment operating components,through graphic display to view the working status of each component of the equipment.
>Ithas the functions of cold trap precooling and vacuum pump preheating,and the time can be set by the userin the system. >Display whether the current running status is normal or not by numerical color.
>Built-in saturated vapor pressure &temperature corresponding relationship table,dearly displaying the corresponding relationship.
o One-key operation,the operation is extremely simple,no special professional learning and training is required.
o Thermal electromagnetic over-current and short-circuit protection,making the equipment run more safely and stably.
o Active reminder of equipment maintenance time and vacuum pump replacement lubricating oil time.
o Ambient temperature monitoring,the device automatically reminds the alarm if the ambient temperature is too high.
o Optional eutectic point detection device and endpointjudgment detection system make freeze-drying more scientific.
Drying chamber | · The separate drying chamber reduces the probability of damage caused byimproper handling of the drying chamber due to height and weight.The upper cover is anodized aluminum with anti-corrosion treatment.●Highly transparent plexiglass drying chamber,good light and heat radiation conduction.Reserve 6/12 external valve ports.· 316L stainless steel all black Teflon coating drying chamber,suitable for drying organic solvents and various corrosive samples,reserved for 6/12 external ports.· 316L stainless steel vertical multi-port multi-manifold external rack(The standard configuration is aplexiglass drying chamber,and one ofthe diying chambers can be optional) |
Cold trap andcondensing coil | · The cold trap cavity and condensation coil are made of 316L stainless steel.Large opening and channel design,the gas can be quickly captured through the condensing coil.· The cavity and condensing coil are equipped with Teflon anti corrosion coating as standard,which can trap organic solvents and various corrosive solvents.· The condensing coil is placed in the cold trap to increase the condensate catchment area,which can effectively prevent external condensation. |
Sample shelves | · 316Lstainless steel stackable sample shelves,capable oflyophilizing machine solvents and various corrosive solvents.Each shelf can be placed and taken freely without manual work.The distance and height ofeach shelfcan be adjusted freely.-All black coating,good heat absorption performance.-Special hole for freeze-drying of centrifuge tubes,which is conducive to the drying of samples in centrifugetube containers.· All-aluminum Teflon surface anti-corrosion treatment electric heating shelf. |
Refrigerationsystem | · Danffos compressorCopper plate heat exchanger,the largest area to increase heat dissipation· Unique refrigeration technology,the temperature of the cold trap can be lowered from ambient temperatureto below-80C°within 2 minutes.· Class A temperature sensor with high temperature accuracy and low error.· Multiple detection and alarm system,the refrigeration system pressure is too high or refrigeration failure cantimely alarm and take corresponding measures. |
Techinical parameter
Item No | ST7003 | ST7006 | ST9006 | |
Ice condenser capacity | 3kg | 6kg | 6kg | |
Ice condenser temperature | -70℃ | -70℃ | -90℃ | |
Cold trap volume | 5L | 10L | 10L | |
Freeze dried chamber | Bell shape (standard configuration)/Multi manifold type/T-shaped frame type | |||
Sample shelf | φ200mm.Single layer area 0.03m2 with 3-5 layers available | ●260mm/485mm.Single layer area 0.055m²/0.185m².3-10 layers/optional ● Electric heating/manual capping/optiona | ||
Vacuum pump | Extreme vacuum 5*10*mbar with a pumping capacity of 8m³/h optional pump of various types | |||
Oil mist filte | Standard configuration | |||
Vacuum pump connection pipe | Single forming stainless steel flexible pipe DN16 ISO-KF L-1.2m | |||
Anti-corrosive treatment | Cold trap,condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents. | |||
External valve | 6pieces/optional | 1-48 pieces/optional | ||
Eutectic point detection system | Support/Optional | |||
Data output analysis | Support/Optional | |||
Powe | 0.6kw | 1.1kw | 1.5kw | |
Size | 420*545*455mm | 510*570*500mm |
Freezer Dryer, 3kgs-6kgs water capturer
Model:
ST7003,ST7006,ST9006
Brand:
Yingtai
These kits are designed for the rapid spin column removal of endotoxins from previously purified DNA. Norgen’s spin columns bind DNA while endotoxins, salts and other contaminants are washed away. These kits reduce endotoxins to 0.1 EU/μg DNA or less providing plasmid DNA that is immediately ready for transfections or other endotoxin-sensitive applications. Typical recovery of DNA is >90% of the starting sample.
Endotoxin Removal Kit (Mini)
This kit is designed for the rapid spin column removal of endotoxins from up to 25 µg of previously purified DNA. The convenient spin column procedure can be completed in approximately 20 minutes.
Endotoxin Removal Kit (Midi)
This kit is designed for the rapid spin column removal of endotoxins from up to 200 μg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.
Endotoxin Removal Kit (Maxi)
This kit is designed for the rapid spin column removal of endotoxins from up to 1 mg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.
About Endotoxins
Endotoxins (also called lipopolysaccharides), are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines. Therefore, the removal of endotoxins from plasmid preparations is often necessary prior to the use of the DNA in such downstream applications.
Figure 1 / 2
Click for expanded view
Kit Specifications | |
Maximum DNA Input | 25 μg |
Final Endotoxin Level | ≤ 0.1 EU/µg DNA |
Size of DNA Purified | Up to 13,000 bp |
Maximum DNA Volume Input | 100 μL |
Average Recovery | > 90% |
Time to Complete 10 Purifications | 20 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Component | Cat. 22700 (25 preps) | Cat. 52200 (10 preps) | Cat. 21900 (4 preps) |
---|---|---|---|
Buffer SK | 15 mL | 60 mL | 60 mL |
Wash Solution H | 18 mL | 18 mL | 18 mL |
Elution Buffer I | 6 mL | 12 mL | 12 mL |
Endotoxin Removal Solution | 1.5 mL | 1.5 mL | 1.5 mL |
Precipitation Solution | – | 1.5 mL | 1.5 mL |
Spin Columns (assembled with Collection Tubes) | – | 10 | 4 |
Spin Columns | 25 | – | – |
Collection Tubes | 25 | – | – |
Elution Tubes (1.7 mL) | 25 | – | – |
Elution Tubes (15 mL) | – | 10 | – |
Elution Tubes (50 mL) | – | – | 4 |
Product Insert | 1 | 1 | 1 |
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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