Campylobacter jejuni is a curved, rod-shaped and microaerophillic gram negative bacterium. It is one of the most common causal agents of gastroenteritis with diarrhea as the main symptom. While infection of C. jejuni is seldom life-threatening, it is considered one of the most common food-borne bacteria with over 2 million people infected per year in US alone. Infection of C. jejuni usually results from consumption of poorly prepared food including undercooked meat (particularly poultry), untreated water or raw unpasteurized milk. Traditional identification of C. jejuni involves culturing, however the microaerophilic characteristic of this bacterium make the enrichment process laborious and costly.
Campylobacter jejuni TaqMan PCR Kit, 100 reactions
Campylobacter jejuni TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM36150 (100 preps) | Cat. TM36110 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| Campylobacter jejuni Primer & Probe Mix | 280 μL | 280 μL |
| Campylobacter jejuni Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
For selective isolation and culture of Staphylococcus aureus.
Peptone and beef extract powder provides carbon, nitrogen, vitamins and minerals; D- mannitol to fermentable sugars; higher levels of sodium chloride to provide a higher osmotic pressure, suppress most non-staphylococcal microorganisms ; phenolsulfonphthalein as pH indicator; agar is medium coagulant. Typical pathogenic staphylococci (coagulase positive) D- mannitol produce acid fermentation and produce yellow colonies with a yellow halo, typically non-pathogenic Staphylococcus unfermented D- mannitol to form red colonies.
Formulation(per liter):
Pancreatic digest of casein 5.0g
Pancreatic digest of animal tissue 5.0g
Beef Extract 1.0g
Sodium Chloride 75.0g
Mannitol 10.0g
Phenol Red 0.025g
Agar 15.0g
Final PH 7.4±0.2
How to use：
1.Suspend 111g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.
Quality control：
| Item | The name and number of strain | Growth | Colony Color |
| 1 | Staphylococcus aureus CMCC (B) 26003 | Good | Golden yellow |
| 2 | Staphylococcus epidermidis CMCC (B) 26069 | Good | Red |
| 3 | Escherichia coli CMCC (B) 44102 | Inhibition | — |
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 250g/bottle
250g
Sulfo DBCO-PEG3-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain and sulfo group increase water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Sulfo DBCO-PEG3-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain and sulfo group increase water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.