DBCO-PEG2-Maleimide is a PEG linker containing a DBCO moiety and a terminal primary maleimide group. The maleimide group will react with a thiol group to form a covalent bond, enabling the connection of biomolecule with a thiol. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG2-Maleimide is a PEG linker containing a DBCO moiety and a terminal primary maleimide group. The maleimide group will react with a thiol group to form a covalent bond, enabling the connection of biomolecule with a thiol. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp (DNA). HiPure Circulating DNA Midi Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine.
This product is for research use only.
Specifications
Features | Specifications |
Main Functions | Isolation circulating DNA from 1-5ml plasma, serum, body fluids using vacuum protocol |
Applications | qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc. |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (vacuum) |
Sample type | Serum, plasma and other cell-free fluid samples |
Sample amount | 1-5ml |
Elution volume | ≥50μl |
Time per run | ≤60 minutes |
Liquid carrying volume per column | 4ml |
Binding yield of column | 1mg |
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer.
Contents | D318201C | D318202C |
Purification Times | 10 | 50 |
Buffer ACL | 50 ml | 250 ml |
Buffer ACB* | 60 ml | 300 ml |
Buffer DCW1* | 4.4 ml | 22 ml |
Buffer DCW2* | 5 ml | 10 ml |
Proteinase K | 110 ml | 540 mg |
Protease Dissolve Buffer | 10 ml | 30 ml |
Carrier RNA | 110 μg | 110 μg |
Nuclease Free Water | 10 ml | 20 ml |
HiPure CFDNA Mini Columns | 10 | 50 |
2 ml Collection Tubes | 20 | 100 |
Extender Tube | 10 | 50 |
Vac-Connector | 10 | 50 |
Storage and Stability
Proteinase K, Carrier RNA should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Experiment Data
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments,
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Specifications
Features | Specifications |
Main Functions | IVD5412 precast kit for MagMix 32, smart 32 |
Applications | RT-PCR,PCR,NGS |
Products | Viral DNA / RNA, body cell DNA / RNA |
Purification method | Polydisperse magnetic beads |
Purification technology | Magnetic beads technolog |
Process method | Manual or automatic |
Sample type | |
Sample amount | 200μl |
Adaptive instrument | Nucleic acid extractor, pipetting workstation |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Nuclease Free Water.
Advantages
Kit Contents
Cat.No | Reagent | IVD5412-TL-96 |
PK/Carrier RNA | 24mg/310μg | |
Protease Dissolve Buffer Blue | 1.8 ml | |
Tip | 12 | |
Prepackage Plate(2.0ml Plate) | Row 1/7:500µl Buffer MLBRow 2/8:500µl Buffer MW1Row 3/9:500µl Buffer CWRow 4/10:500µl Buffer CW&MPNRow 5/11:100µl Buffer AVE | 6 |
Storage and Stability
This kit is shipped and stored at room temperature and is valid for 12 months.
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
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