Norgen’s Cells and Tissue DNA Isolation Kits are designed for the rapid preparation of genomic DNA from cultured cells as well as various tissue samples and urine. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with PCR and Southern Blot analysis.
Cells and Tissue DNA Isolation Kit (Spin Column)
Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The protocol can be completed in approximately 30 minutes for cells and within 90 minutes for tissues. Each kit contains sufficient materials for 50 preparations.
Cells and Tissue DNA Isolation Micro Kit (Micro)
Optimized for small inputs of cells and tissues, such as Laser-Captured Microdissection (LCM). Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. Preparation time for a single sample is approximately 60 minutes, and each kit contains sufficient materials for 50 preparations.
Cells and Tissue DNA Isolation Kit (Magnetic Bead System)
Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. Preparation for 10 purifications is approximately 40 minutes of hands-on time.
Cells and Tissue DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. The Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) also can be integrated with a robotic automation system.
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| Kit Specifications | |
| Maximum Input | 20 mg of animal tissue Up to 150 μL of viral suspension 3 x 106 cells |
| Column Binding Capacity | > 50 μg |
| Average Yield | 8 μg (from 1 x 106 HeLa Cells) 10 μg (from 10 mg kidney) |
| Elution Volume | 50 – 200 μL |
| Analyte Purified | Genomic DNA, mitochondrial DNA, viral DNA |
| Format | Spin Column |
| Time to Complete 10 Purifications | 30 min (cells) and 90 min (tissue) |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
| Component | Cat. 53100 (50 preps) | Cat. 57300 (50 preps) | Cat. 59100 (50 preps) | Cat. 62500 (192 preps) |
|---|---|---|---|---|
| Lysis Buffer B | 20 mL | 20 mL | 20 mL | 1 x 40 mL 1 x 20 mL |
| Solution WN | 18 mL | 18 mL | 18 mL | 55 mL |
| Wash Solution A | 18 mL | 18 mL | – | – |
| Elution Buffer B | 30 mL | 8 mL | 15 mL | 1 x 30 mL 1 x 15 mL |
| Proteinase K | 1.2 mL | 1.2 mL | 1.2 mL | – |
| Proteinase K in Sotrage Buffer | – | – | – | 4 mL |
| Magnetic Bead Suspension | – | – | 2 x 1.1 mL | 8.5 mL |
| 96-Well Plate | – | – | – | 2 |
| Spin Columns | 50 | – | – | – |
| Micro Spin Columns | – | 50 | – | – |
| Collection Tubes | 50 | 50 | – | – |
| Elution Tubes (1.7 mL) | 50 | 50 | 50 | – |
| 96-Well Elution Plate | – | – | – | 2 |
| Adhesive Tape | – | – | – | 2 |
| Product Insert | 1 | 1 | 1 | 1 |
Folic acid, is one of the B vitamins used to treat anemia caused by folic acid deficiency.
This kit is a product based on indirect-competitive ELISA, which is fast, easy, accurate and sensitive compared with common instrumental analysis.
Detection limit if folic acid in liquid samples
Highly Sensitive, rapid, robust screening kit for Folic Acid
Product Details
Sample Type:
Serum, Plasma, Whole Blood
Package Size:
48 Tests/Kit
Test Time:
5-20 Minutes
Target Disease:
African Swine Fever Virus
Product Name:
African Swine Fever Virus Isothermal Detetction Fluorescence Kit
Test Principle:
Nucleic Acid Detection
Target Species:
Pigs
High Light:
,
,
Product Description
Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection
Product parameters:
| Kit composition (WLRE8208KIT 48T/Pack) | |
| Composition | Content |
| E buffer | 1 mL×2 Tubes |
| B buffer | 150 μL×1Tube |
| Positive control template | 100 μL×1Tube |
| Reagents | 48T |
Product features and advantages:
| Product features and advantages | |
| Features | Advantages |
| High sensitivity | 1 copies/ml |
| Strong specificity | Specificity is superior to PCR |
| Short reaction time | 20min |
| Polymorphic reagent | Liquid, freeze-dried powder, freeze-dried ball |
| Easy to operate | Few steps of liquid dispensing; It is even possible to add only samples and amplify to get the resultsDesign of primers and probe is simple |
| Easy to store and transport | It is best preserved at -20℃, and can be stored at room temperature for up to 30 days in a cool place away from light.The freeze-dried form has a long storage time and is convenient for transportation |
| Low requirements on equipment | Applicable to Applied Biosystems 7500, QuantStudio 3/5, QuantStudio 6/7 Flex fluorescence quantitative PCR instrument;Applicable to our WL-16-III and other isothermal fluorescence detector |
Product application:
| Application | |
| Ultra-clean laboratory | African Swine Fever Virus Isothermal Detection |
| Indoor | |
Operation procedure and process:
| Operation procedure and process: |
| Take out the liquid components of the kit in advance, thaw at room temperature, and shake to mix. |
| Prepare freeze-dried reagents according to the number of samples to be tested, negative and positive controls, and add 37.5 μL E buffer to each freeze-dried reagent. |
| Select the appropriate nucleic acid extraction method and reagent to extract the sample nucleic acid |
| Add 10 μL nucleic acid template to the reaction tube (the amount of template can be adjusted to be filled with sterile water; That is, 10 μL nucleic acid template plus sterile water), 10 μL positive control template was added to positive control, and 10 μL sterile water was added to negative control |
| Add 2.5μL B buffer to each reaction tube and close the tube cap (for multiple reactions, it is recommended to add B buffer to the inside of the tube cap) |
| Thoroughly mix the reaction tube upside and down for 8-10 times. After mixing, shake (or rapidly centrifuge) the reaction liquid to the bottom of the tube and transfer it to the amplification zone. |
Performence Comparison Data:
| No. | TT value | Template concentration | Result determination |
| 1 | 4.2 | 10-1 | Positive |
| 2 | 5.5 | 10-2 | Positive |
| 3 | 10.2 | 10-3 | Positive |
| 4 | 17.0 | 10-4 | Positive |
| 5 | 12.0 | 10-5 | Positive |
| 6 | – | 10-6 | Negative |
| 7 | – | 10-7 | Negative |
| 8 | Negative control | Negative | |
Support and Services:
Livestock Disease Kit Technical Support and Services
We provide technical support and services for our Livestock Disease Kit. We are committed to helping you get the most out of your product and ensuring your satisfaction with our products and services.
If you have any questions or need technical support, please do not hesitate to contact us. We are here to help you make the most of your Livestock Disease Kit.
FAQ:
Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection