Product Details
Sample Type:
Serum, Plasma, Whole Blood
Package Size:
48 Tests/Kit
Test Time:
5-20 Minutes
Target Disease:
African Swine Fever Virus
Product Name:
African Swine Fever Virus Isothermal Detetction Fluorescence Kit
Test Principle:
Nucleic Acid Detection
Target Species:
Pigs
High Light:
,
,
Product Description
Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection
Product parameters:
Kit composition (WLRE8208KIT 48T/Pack) | |
Composition | Content |
E buffer | 1 mL×2 Tubes |
B buffer | 150 μL×1Tube |
Positive control template | 100 μL×1Tube |
Reagents | 48T |
Product features and advantages:
Product features and advantages | |
Features | Advantages |
High sensitivity | 1 copies/ml |
Strong specificity | Specificity is superior to PCR |
Short reaction time | 20min |
Polymorphic reagent | Liquid, freeze-dried powder, freeze-dried ball |
Easy to operate | Few steps of liquid dispensing; It is even possible to add only samples and amplify to get the resultsDesign of primers and probe is simple |
Easy to store and transport | It is best preserved at -20℃, and can be stored at room temperature for up to 30 days in a cool place away from light.The freeze-dried form has a long storage time and is convenient for transportation |
Low requirements on equipment | Applicable to Applied Biosystems 7500, QuantStudio 3/5, QuantStudio 6/7 Flex fluorescence quantitative PCR instrument;Applicable to our WL-16-III and other isothermal fluorescence detector |
Product application:
Application | |
Ultra-clean laboratory | African Swine Fever Virus Isothermal Detection |
Indoor |
Operation procedure and process:
Operation procedure and process: |
Take out the liquid components of the kit in advance, thaw at room temperature, and shake to mix. |
Prepare freeze-dried reagents according to the number of samples to be tested, negative and positive controls, and add 37.5 μL E buffer to each freeze-dried reagent. |
Select the appropriate nucleic acid extraction method and reagent to extract the sample nucleic acid |
Add 10 μL nucleic acid template to the reaction tube (the amount of template can be adjusted to be filled with sterile water; That is, 10 μL nucleic acid template plus sterile water), 10 μL positive control template was added to positive control, and 10 μL sterile water was added to negative control |
Add 2.5μL B buffer to each reaction tube and close the tube cap (for multiple reactions, it is recommended to add B buffer to the inside of the tube cap) |
Thoroughly mix the reaction tube upside and down for 8-10 times. After mixing, shake (or rapidly centrifuge) the reaction liquid to the bottom of the tube and transfer it to the amplification zone. |
Performence Comparison Data:
No. | TT value | Template concentration | Result determination |
1 | 4.2 | 10-1 | Positive |
2 | 5.5 | 10-2 | Positive |
3 | 10.2 | 10-3 | Positive |
4 | 17.0 | 10-4 | Positive |
5 | 12.0 | 10-5 | Positive |
6 | – | 10-6 | Negative |
7 | – | 10-7 | Negative |
8 | Negative control | Negative |
Support and Services:
Livestock Disease Kit Technical Support and Services
We provide technical support and services for our Livestock Disease Kit. We are committed to helping you get the most out of your product and ensuring your satisfaction with our products and services.
If you have any questions or need technical support, please do not hesitate to contact us. We are here to help you make the most of your Livestock Disease Kit.
FAQ:
Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection
Overview
Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the utter is mainly caused by infection of various bacteria. Streptococcus uberis is a gram-positive bacterium that is known worldwide as an environmental pathogen responsible for a high proportion of cases of mastitis in lactating cows and is also the predominant organism isolated from mammary glands during the non-lactating period. Often it is resistant to treatment and causes persistent high somatic cell counts without clinical mastitis.
Streptococcus uberis TaqMan PCR Kit, 100 reactions
Streptococcus uberis TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
Figure 1 / 3
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
Component | Cat. TM30850 (100 preps) | Cat. TM30810 (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
S. uberis Primer & Probe Mix | 280 μL | 280 μL |
S. uberis Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |
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