DBCO-PEG2-DBCO is a PEG linker containing two terminal DBCO groups. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain allows for increased water solubility. T Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG2-DBCO is a PEG linker containing two terminal DBCO groups. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain allows for increased water solubility. T Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Culture Plate 24 Wells
Cell Culture Plate provide excellent smoothness and uniformity based on accurate molding technology. By such property, clear view can be available when examine with a microscope.
Specifications: 6wells
12wells, 24wells., 48wells, 96wells
PRODUCT FEATURES
For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.
For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.
Cell Culture Plate provide excellent smoothness and uniformity based on accurate molding technology. By such property, clear view can be available when examine with a microscope.
Specifications: 6wells
12wells, 24wells., 48wells, 96wells
Norgen’s EXTRAClean Urine Exosome Purification and RNA Isolation Mini Kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of RNA from different urine sample volumes ranging from 250 μL to 1 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The purified RNA is free from any protein-bound circulating RNA and of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, NGS application, Northern blotting, RNase protection and primer extension, and expression array assays.
Figure 1 / 9
Click for expanded view
| Minimum Urine Input | 250 μL |
| Maximum Urine Input | 1 mL |
| Size of Exosomes Purified | 40 nm – 150 nm |
| Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
| Elution Volume | 50-100 μL |
| Time to Complete 10 Purifications | 35-40 minutes |
| Average Yields | Variable depending on specimen |
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.