The Norgen 1 kb RNA Ladder is a set of RNA transcripts derived from recombinant DNA templates. This ladder is suitable for precise sizing of a wide range of RNA molecules using native or denaturing agarose gels, and can be visualized under UV.
Contents:
• 2 vials of lyophilized RNA ladder (25 loads each)
• 1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution
• 2x loading buffer
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Specifications:
• Nine discrete bands, ranging from 200 to 4,000 bases
• Higher intensity band at 1,000 bases for easy reference
Contents:
• 2 vials of lyophilized RNA ladder (25 loads each)
• 1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution
• 2x loading buffer
Instructions:
To reconstitute the lyophilized RNA ladder, add 250 µL of 1x loading buffer to each 25 loads vial and vortex gently.
Heat at 80°C for 10 minutes. Incubate on ice for 1 min. Load 10 µL on a 1-2% gel. For optimal results, use Norgen 2x loading buffer with each RNA sample. No need for staining and destaining of denaturing gels since Norgen’s loading buffer contains ethidium bromide.
| Clone | IHC615 |
| Source | Mouse Monoclonal |
| Positive Control | Tonsil, Follicular Lymphoma, Diffuse Large B-Cell Lymphoma |
| Dilution Range | 1:200 |
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DBCO-PEG4-C3-sulfonic acid serves as a bifunctional PEG linker. The DBCO click chemistry handle conjugates with azides on target molecules to form stable triazole linkages. The sulfonic acid can participate in esterification, halogenation and displacement reactions.
DBCO-PEG4-C3-sulfonic acid serves as a bifunctional PEG linker. The DBCO click chemistry handle conjugates with azides on target molecules to form stable triazole linkages. The sulfonic acid can participate in esterification, halogenation and displacement reactions.