The kit is developed for RNA quantification. The RNA Quantification HS kit includes HS Dye, HS Dilution Buffer, and two RNA Standards. Simply dilute the HS Dye with the HS Dilution Buffer, add RNA sample, then read the concentration using the Qubit Fluorometer. The assay is accurate for RNA concentrations from 250 pg/µL to 100 ng/µL (Figure 1). The RNA Quantification High Sensitivity Kit has several advantages over traditional RNA quantitation such as stability, sensitivity, and contaminant tolerance.
Features
The performance of the BioDynami RNA Quantification HS kit is nearly identical to that of Thermo Fisher’s Qubit RNA HS kit (figure below).
Common contaminants such as detergents, solvents, salts, free nucleotides, or protein are well tolerated in the assay (Table 1).
Qubit is a registered trademark of Thermo Fisher Scientific.
The kit is developed for RNA quantification. The RNA Quantification HS kit includes HS Dye, HS Dilution Buffer, and two RNA Standards. Simply dilute the HS Dye with the HS Dilution Buffer, add RNA sample, then read the concentration using the Qubit Fluorometer. The assay is accurate for RNA concentrations from 250 pg/µL to 100 ng/µL (Figure 1). The RNA Quantification High Sensitivity Kit has several advantages over traditional RNA quantitation such as stability, sensitivity, and contaminant tolerance.
This product allows rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples. Up to 100mg stool sample and 500 mg soil samples can be processed in 60 minute. The system combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of spin column technology to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed inparallel.
Specifications
| Features | Specifications |
| Main Functions | Isolation DNA from 200-500mg soil, 50-100mg stool, or 100-500mg other environmental samples using 96 plate |
| Applications | PCR, southern blot and enzyme digestion, etc. |
| Purification method | 96 well plate |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Soil, stool, other environmental samples |
| Sample amount | Soil: 200-500mgStool: 50-100mgOther environmental samples: 100-500mg |
| Elution volume | |
| Time per run | |
| Liquid carrying volume per column | |
| Binding yield of column |
Soil/Stool sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteins, polysaccharides, and other contaminants are removed using our proprietary Absorber Solution. Binding conditions are then adjusted and the sample is applied to a DNA Mini Column. Two rapid wash steps remove trace contaminants and pure DNA is eluted in low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.
Kit Contents
| Contents | D314401 | D314402 | D314403 |
| Purification Times | 1 x 96 Preps | 4 x 96 Preps | 20 x 96 Preps |
| HiPure DNA Plate | 1 | 4 | 20 |
| 96 Well Plate (2.2ml) | 1 | 4 | 20 |
| 1.6ml Collection Plate | 1 | 4 | 20 |
| 0.8ml Collection Plate | 1 | 4 | 20 |
| 2ml Bead Tubes | 100 | 400 | 2000 |
| Buffer SOL | 100 ml | 360 ml | 2 x 900 ml |
| Buffer SDS | 10 ml | 36 ml | 180 ml |
| Reagent DX | 1 ml | 1.8 ml | 9 ml |
| Buffer PS | 20 ml | 80 ml | 400 ml |
| Absorber Solution | 20 ml | 80 ml | 400 ml |
| Buffer GDP | 150 ml | 500 ml | 3 x 900 ml |
| Buffer GW2* | 50 ml | 100 ml | 4 x 200 ml |
| Buffer AE | 30 ml | 120 ml | 500 ml |
Storage and Stability
Absorber Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
This product allows rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples. Up to 100mg stool sample and 500 mg soil samples can be processed in 60 minute. The system combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of spin column technology to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed inparallel.
The Residual DNA Sample Preparation Kit uses chemical lysis and magnetic beads to extract DNA from diverse sample types, including samples that contain high protein and low DNA concentration. The kit extracts residual genomic DNA from products that are produced in cell lines such as CHO, E. coli, E1A, HEK293, Vero, NS0 and Baculovirus. For quantification of residual DNA, we recommend using the resDNASEQ Residual DNA Quantitation kit as described in the resDNASEQ Residual DNA Quantitation kit User Guide. To ensure accurate quantitative results, each sample in triplicate and perform a single PCR reaction for each extraction.
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Only three steps will be need for Sample Preparation，and the total time of Sample preparation is about 30 minites.
The good Recovery rate of Sample Preparation.
All components of the Sample Preparation Kit can be stored at room temperature.
Note: Price not include shipment & duty, contact us to get full quote.
The Residual DNA Sample Preparation Kit uses chemical lysis and magnetic beads to extract DNA from diverse sample types, including samples that contain high protein and low DNA concentration. The kit extracts residual genomic DNA from products that are produced in cell lines. The operation is easier and the higher nucleic acid yield is guaranteed at the same time.