For rapid, sensitive and accurate screening of potential Tyrosinase inhibitors
Tyrosinase (EC 1.14.18.1) is a copper-binding enzyme that is expressed across a vast range of species ranging from bacteria and fungi to mammals. It is involved in two sequential reactions of the melanin synthesis pathway: first being the hydroxylation of a monophenol and second the conversion of an ortho-diphenol to a quinone. Quinone then undergoes a series of reactions including polymerization to form melanin.
Tyrosinase is of great interest to the agriculture industry since it causes browning of fruits, vegetable, and mushrooms, as well as to the cosmetic industry as it causes skin darkening. Development and screening of tyrosinase inhibitors, therefore, is very useful for conditions such as hyperpigmentation and melasma. Tyrosinase activity is significantly increased in melanoma. Therefore, the detection of tyrosinase activity could be promising as a specific diagnostic test for melanoma and may be useful in monitoring patient response to melanoma treatments.
This Tyrosinase Activity Assay Kit is a simple one-step, plate-based assay for the measurement of tyrosinase activity in biological samples. In this assay, tyrosinase catalyzes the conversion of a phenolic substrate to a Quinone intermediate, which reacts with the tyrosine enhancer forming a highly stable chromophore with absorbance at 520 nm. The assay can detect as low as 30 μU Tyrosinase in biological samples.
Highly reproducibility
Highly Sensitive Assay to Screen for Tyrosinase Activity
Stable formulation of ready to use Reaction Facilitator (tyrosinase)
Giardiasis is a disease of the small bowel caused by the protozoan parasite Giardia intestinalis (syn.duodenalis or lamblia). Giardia is one of the most common intestinal parasites in the world, and occurs at very high prevalence rates in places with poor water sanitation. Individuals become infected through ingesting or coming into contact with contaminated water, food or soil. It can also be spread through the faecal-oral route due to poor hygiene practices, which makes it common in day-care centers. G. intestinalis lives inside the intestines of infected humans or other animals including cats, dogs, birds, cows, beaver and deer. Symptoms of infection include diarrhea, malaise, excessive gas, bloating, nausea, diminished interest in food, possible vomiting and weight loss.
There are 2 kits available for the detection of Giardia intestinalis:
Giardia intestinalis TaqMan RT-PCR Kit, 100 reactions
Giardia intestinalis TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
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Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM43850 (100 preps) | Cat. TM43810 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| Giardia intestinalis Primer & Probe Mix | 280 μL | 280 μL |
| Giardia intestinalis Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
Tetra-(amido-PEG10-propargyl) is a branched crosslinker with four terminal propargyl groups. The propargyl groups can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage.
Tetra-(amido-PEG10-propargyl) is a branched crosslinker with four terminal propargyl groups. The propargyl groups can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage.