
Opentrons 4-in-1 Tube Rack set includes 2 base stands and 4 tube holder tops: 1.5ml, 2ml, 15ml + 50ml, 15ml, and 50ml. The tube holder tops snap securely on the base stand, which fits directly on the deck. The Tube Rack Set is not autoclavable.
Opentrons 4-in-1 Tube Rack set includes 2 base stands and 4 tube holder tops: 1.5ml, 2ml, 15ml + 50ml, 15ml, and 50ml. The tube holder tops snap securely on the base stand, which fits directly on the deck. The Tube Rack Set is not autoclavable.
Opentrons 4-in-1 Tube Rack set includes 2 base stands and 4 tube holder tops: 1.5ml, 2ml, 15ml + 50ml, 15ml, and 50ml. The tube holder tops snap securely on the base stand, which fits directly on the deck. The Tube Rack Set is not autoclavable.
Grey, standard profile semi-skirted PCR plate with a rigid two-component hardshell design which eliminates warping and distortion during PCR testing.
2 Layer Cell Factory Double Narrow Mouth
Huayi Cell Factory is a robust and easy-to-use cell culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.
To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.
Specifications: 1 layer, 2 layers, 5 layers, 10 layers and 40 layers.
PRODUCT FEATURES
of one 10-layer Cell Factory unit is equivalent to the area
of 36 T-175 flasks
Huayi Cell Factory is a robust and easy-to-use cell culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.
To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.
Specifications: 1 layer, 2 layers, 5 layers, 10 layers and 40 layers.
The HiPure Midi system provides a fast, simple, and cost-effective plasmid DNA midiprep method for routine molecular biology laboratory applications. HiPure Midiprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Specifications
Features | Specifications |
Main Functions | Isolation up to 500µg endotoxin-free plasmid DNA from 25-50ml bacterial culture. Recommend for High copy vector, Low elution volume, High concentration |
Applications | Cell transfection, animal injection, etc. |
Purification method | Midi spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | High copy plasmid vector |
Sample amount | 25-50ml LB |
Yield | 10-500μg |
Elution volume | ≥0.1ml |
Time per run | ≤60 minutes |
Liquid carrying volume per column | 4ml |
Binding yield of column | 250μg |
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method,then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
Kit Contents
Contents | P123102 | P123102B |
Purification Times | 50 Preps | 50 Preps |
RNase A | 30 mg | 30 mg |
Buffer E1 | 150 ml | 150 ml |
Buffer E2 | 150 ml | 150 ml |
Buffer E3 | 150 ml | 150 ml |
Buffer E4 | 150 ml | 150 ml |
Buffer E5 | 150 ml | 150 ml |
Buffer PW2* | 50 ml | 50 ml |
Elution Buffer | 30 ml | 30 ml |
MaxPure EF Mini Column | 50 | 50 |
2 ml Collection Tubes | 50 | 50 |
Lysate Clear Midi Syringe | 50 | 50 |
Extend Tubes | 50 | 50 |
50ml Centrifuge Tubes | 50 | |
Support Tubes | 50 |
Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A and optional LyseBlue reagent, Buffer P1 is stable for 6 months when stored at 2–8°C.
For any technical problems or customized products, please contact us.
F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here
The HiPure Midi system provides a fast, simple, and cost-effective plasmid DNA midiprep method for routine molecular biology laboratory applications. HiPure Midiprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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