Huayi Cell Factory is a robust and easy-to-use cell
culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.
To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.
Huayi Cell Factory is a robust and easy-to-use cell
culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.
To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.
The product assembled with ultrasonic welded technology
Versatile port design facilitates both pouring and aseptic filling techniques
Gamma radiation sterilization
the cell culture surface area
of one 10-layer Cell Factory unit is equivalent to the area
of 36 T-175 flasks
Other Products
14 Month Validity Isothermal Amplification Kit NFO Keep Away From Light Keep Away From Light
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Product Description
Product Detail
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).
Technical Parameters:
Parameters
Details
Product Name
DNA Isothermal Amplification Kit NFO
Manufacturer
Amp-future
Storage Temperature
-20°C
Kit Components
Enzymes, Buffers ,Reagents
Packaging
48 Tests/box
Detection Limit
500-1000copies/µL
Shipping
ICE
Test Time
5-20mins
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Isothermal nucleic acid Applications
Suitable for DNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
This product provides high quality purification of total DNA from whole blood, plasma, serum, buffy coat, or other body fluids, lymphocytes and cultured cells. There is no need to use toxic phenol chloroform extraction or time-consuming alcohol precipitation. The extraction process finish in 60 minutes. Purified DNA includes genomic DNA, mitochondrial DNA, viral DNA (e.g. HBV), or DNA from other parasitic microorganisms. The obtained DNA can be directly used in PCR, viral DNA detection and other experiments.
This kit can use on manual protocol or 96 channel automated extraction system.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 200μl whole blood
Applications
PCR, southern bolt and virus detection, etc
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells
Sample amount
200μl
Elution volume
≥50μl
Time per run
≤60 minutes
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
High binding force – suitable for handling DNA rich samples, such as whole blood, buffy coat, concentrated blood, etc.
Fast – polydisperse magnetic beads, fast magnetic response and short extraction time
High purity – the obtained DNA can be directly used for second-generation sequencing, PCR based detection, gene bank, etc.
Automatic – saving time and labor and safer
Kit Contents
Contents
D631101
D631102
D631103
Purification Times
48
96
480
MagPure Particles
1.2 ml
2.5 ml
11 ml
Proteinase K
24 mg
50 mg
220 mg
Protease Dissolve Buffer
1.8 ml
5 ml
15 ml
Buffer AL
15 ml
30 ml
120 ml
Buffer GW1*
22 ml
53 ml
220 ml
Elution Buffer
15 ml
30 ml
100 ml
Storage and Stability
Proteinase K, MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
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This product provides high quality purification of total DNA from whole blood, plasma, serum, buffy coat, or other body fluids, lymphocytes and cultured cells. There is no need to use toxic phenol chloroform extraction or time-consuming alcohol precipitation. The extraction process finish in 60 minutes. Purified DNA includes genomic DNA, mitochondrial DNA, viral DNA (e.g. HBV), or DNA from other parasitic microorganisms. The obtained DNA can be directly used in PCR, viral DNA detection and other experiments.
This kit can use on manual protocol or 96 channel automated extraction system.
96-Well ring magnetic plate with integrated cushion base
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Our most popular ring magnet plate. All of the features of our T480 magnet above with the added security of a cushion base For faster separations you can upgrade to the MSP500R which comes with all of the same features, just stronger magnets Like our ring magnet plates above, this plate comes in two magnet strengths. Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
Our most popular ring magnet plate. All of the features of our T480 magnet above with the added security of a cushion base For faster separations you can upgrade to the MSP500R which comes with all of the same features, just stronger magnets Like our ring magnet plates above, this plate comes in two magnet strengths. Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
