This product allows rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples. Up to 100mg stool sample and 500 mg soil samples can be processed in 60 minute. The system combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of spin column technology to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed inparallel.
Specifications
Features | Specifications |
Main Functions | Isolation DNA from 200-500mg soil, 50-100mg stool, or 100-500mg other environmental samples using 96 plate |
Applications | PCR, southern blot and enzyme digestion, etc. |
Purification method | 96 well plate |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Soil, stool, other environmental samples |
Sample amount | Soil: 200-500mgStool: 50-100mgOther environmental samples: 100-500mg |
Elution volume | |
Time per run | |
Liquid carrying volume per column | |
Binding yield of column |
Soil/Stool sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteins, polysaccharides, and other contaminants are removed using our proprietary Absorber Solution. Binding conditions are then adjusted and the sample is applied to a DNA Mini Column. Two rapid wash steps remove trace contaminants and pure DNA is eluted in low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.
Kit Contents
Contents | D314401 | D314402 | D314403 |
Purification Times | 1 x 96 Preps | 4 x 96 Preps | 20 x 96 Preps |
HiPure DNA Plate | 1 | 4 | 20 |
96 Well Plate (2.2ml) | 1 | 4 | 20 |
1.6ml Collection Plate | 1 | 4 | 20 |
0.8ml Collection Plate | 1 | 4 | 20 |
2ml Bead Tubes | 100 | 400 | 2000 |
Buffer SOL | 100 ml | 360 ml | 2 x 900 ml |
Buffer SDS | 10 ml | 36 ml | 180 ml |
Reagent DX | 1 ml | 1.8 ml | 9 ml |
Buffer PS | 20 ml | 80 ml | 400 ml |
Absorber Solution | 20 ml | 80 ml | 400 ml |
Buffer GDP | 150 ml | 500 ml | 3 x 900 ml |
Buffer GW2* | 50 ml | 100 ml | 4 x 200 ml |
Buffer AE | 30 ml | 120 ml | 500 ml |
Storage and Stability
Absorber Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
This product allows rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples. Up to 100mg stool sample and 500 mg soil samples can be processed in 60 minute. The system combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of spin column technology to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed inparallel.
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasmausually as short fragments, <1000bp(DNA). HiPure Circulating DNA Midi Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. The extracted products can be used for clinical in vitro detection.
Specifications
Features | Specifications |
Main Functions | Isolation circulating DNA from 1-5ml plasma, serum, bodyfluids by spin |
Applications | qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc. |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation) |
Sample type | Serum, plasma and other cell-free fluid samples |
Sample amount | 1-5ml |
Elution volume | ≥50μl |
Time per run | ≤70 minutes |
Liquid carrying volume per column | 4ml |
Binding yield of column | 1mg |
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption plate and filter column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10 Mm Tris,pH 8.0).
Kit Contents
Contents | D318203D |
Purification Times | 50 Preps |
Buffer ACL | 250 ml |
Buffer ACB* | 300 ml |
Buffer DCW1* | 22 ml |
Buffer DCW2* | 10 ml |
Proteinase K | 540 mg |
Protease Dissolve Buffer | 30 ml |
Carrier RNA | 110 μg |
Nuclease Free Water | 20 ml |
HiPure CFDNA Mini Columns | 50 |
2 ml Collection Tubes | 100 |
Extender Tubes | 50 |
Sealing O-Ringes | 50 |
50ml Collection Tube | 50 |
Support Tube | 50 |
Storage and Stability
Proteinase K, Carrier RNA should be stored at 2-8°C upon arrival. However, short-term storage (up to 12weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can bestored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasmausually as short fragments,
The kit provides rapid, simple, and reliable components for the labeling proteins with biotin for use in lateral flow procedures. Kit enables the biotinylation of antibody or proteins to be used with the Universal Lateral Flow Kits (AU2034, AU2037 or AUAU2044 in addition to Streptavidin Colloidal (AU2017) gold or Streptavidin CdZnS Quantum Dots (AU2043).
Attogene’s Protein Biotinylation kit provides the user with reagents for labeling proteins
Desalting columns for purifying the labeled molecule
Convenient and easy to interpret instructions on how to carry out the labeling process.
Made by Attogene in the USA
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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