K-MBG4
SKU: 700004319
100 assays (manual) / 400 assays (auto-analyser)
| Content: | (Malt β-glucanase) 100 assays (manual) / 400 (auto-analyser) Or (Lichenase) 100 / 200 assays (manual) / 330 (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | β-Glucanase/Lichenase |
| Assay Format: | Spectrophotometer, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 400 |
| Signal Response: | Increase |
| Limit of Detection: | (Malt β-glucanase) 4.3 x 10-4 U/mL Or (Lichenase) 9.1 x 10-5 U/mL |
| Reproducibility (%): | ~ 3% |
| Total Assay Time: | (Malt β-glucanase) ~ 20 min Or (Lichenase) ~ 10 min |
| Application examples: | Crude malt extracts, industrial enzyme preparations. |
| Method recognition: | Novel method |
The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.
Mixed linkage β-glucanase (endo-1,3:1,4-β-glucanase) / lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).
Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g. Trichoderma sp.) but this does not result in an increase in absorbance.
Discover more assay kits for enzyme activity measurement.
Data calculators are located in the Documents tab.
Advantages
The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.
DBCO-PEG4-TFP ester serves as a heterobifunctional PEG linker containing an azide-reactive DBCO click chemistry handle and an amine-reactive TFP ester that is less susceptible to undergo hydrolysis compared to NHS ester. The PEG spacer enhances the water-solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG4-TFP ester serves as a heterobifunctional PEG linker containing an azide-reactive DBCO click chemistry handle and an amine-reactive TFP ester that is less susceptible to undergo hydrolysis compared to NHS ester. The PEG spacer enhances the water-solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
t-Boc-N-Amido-PEG7-propargyl is a crosslinking reagent that enables the formation of triazole linkage with azides under the catalyzation of copper. The Boc-protected amine can be deprotected under mild acidic conditions. By introducing PEG chain into the molecule, the hydrophilicity can be greatly improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
t-Boc-N-Amido-PEG7-propargyl is a crosslinking reagent that enables the formation of triazole linkage with azides under the catalyzation of copper. The Boc-protected amine can be deprotected under mild acidic conditions. By introducing PEG chain into the molecule, the hydrophilicity can be greatly improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.