The RNA Seq Library Prep Kit was developed for construction of high quality NGS libraries for next generation sequencing (illumina and MGI Platforms). RNA Sequencing is a very powerful tool to analyze transcriptome such as gene expression and transcription regulation, splicing characterization, mutation and variation detection etc. The kit needs purified RNA (example: rRNA depleted RNA or polyA mRNA) as input for library construction. Library multiplexing is possible with different types of indexes.
RNA Seq Library Prep Kit Workflow
Three index types are available for the illumina platform kits:
Non-index (illumina Cat.# 30055): Libraries do not have index.
Index (illumina Cat.# 30056): A unique barcode sequence with 6 bases has been included in each of the index primers. RNA Sequencing library multiplexing is possible with up to 48 samples. Index information can be downloaded here.
Unique dual index (illumina Cat.# 30057): RNA Sequencing library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a 4-Base Difference Index System. The system can generate indexes with at least 4 bases different from others in the 8-base indexing region. the unique dual indexing primers identify sequencing errors such as index hopping, mis-assignment, and de-multiplexing errors. Index information can be downloaded here.
Indexes are available for the MGI platform kits (Cat.# 34112).
Features
Comparison of the protocol time: BioDynami RNA Seq Library Prep Kit vs other vendors’ kits. Hands-on time and walk-away time were indicated.
Library size and distribution of BioDynami kit, 20 ng and 3 ng of polyA mRNA as input.
Comparison of library yield and duplication rate under the same condition: 20 ng and 3 ng of polyA mRNA were used as input. PCR cycle numbers were indicated.
Comparison of coverage of transcripts: 20 ng of polyA mRNA were used as input. BioDynami kit has more uniform coverage
Blank lateral flow strips are the perfect companion during the development and feasibility testing of a lateral flow device. IN THE BEGINNING, the capture and test line reagents can be spotted onto the nitrocellulose portion of the blank strips at different concentrations, volumes and buffer conditions. This enables the user to determine the optimal conditions for subsequent spraying and enables the user to verify the test and controls will respond according to expectations before scaling up the process.
These blank strips are compatible with all of our colloidal gold products AND:
Lateral flow cassettes: C02022
Streptavidin CdSe/ZnS Quantum Dots For Lateral Flow: AU2043
Streptavidin Europium Chelate Microspheres For Lateral Flow: AU2050
Streptavidin Colloidal Gold For Lateral Flow: AU2017
RNase-Free Lateral Flow Assay Running Buffer Screening Pack: AU2035
Twenty 4.5X60mm strips of each combination of nitrocellulose (4X), wicking material (2X), and sample pad (3X)
A total of 480 strips are provided
100 cassettes
125mL of buffer
K-LACSU
100 assays of each per kit
| Content: | 100 assays of each per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | D-Glucose, Lactose, Sucrose |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 510 |
| Signal Response: | Increase |
| Limit of Detection: | 100 mg/L |
| Reaction Time (min): | ~ 60 min |
| Application examples: | Flours, beverages, dairy products, milk, foodstuffs containing milk, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Used and accepted in food analysis |
This product has been discontinued (read more).
The Lactose/Sucrose/D-Glucose assay kit is suitable for the measurement and analysis of sucrose, lactose and D-glucose in flour mixtures and other materials.
Explore our full range of mono/disaccharide assay kits.
Advantages
The Lactose/Sucrose/D-Glucose assay kit is suitable for the measurement and analysis of sucrose, lactose and D-glucose in flour mixtures and other materials.