AZscript™ Reverse Transcriptase (RT) is an RNA-dependent DNA polymerase used to synthesize complementary DNA (cDNA) from an RNA template.
While originating from Moloney Murine Leukemia Virus (M-MLV), AZscript RT has been engineered for increased thermostability and reduced RNase H activity.
In cDNA synthesis a higher reaction temperature can reduce RNA secondary structures and improve efficiency and specificity of the reaction.
Furthermore, a reduction in RNase H activity increases performance of the cDNA synthesis, especially for longer transcripts.
The combination of these tailored features ensures that AZscript Reverse Transcriptase provides an effective and reliable solution for various cDNA synthesis applications.
AZscript™ Reverse Transcriptase (RT) is an RNA-dependent DNA polymerase used to synthesize complementary DNA (cDNA) from an RNA template.
For enriching bile-tolerant Gram-negative bacteria in the microbiological examination of pharmaceutical products.
Pancreatic digest of gelatin provide protein, vitamins and amino acids; glucose carbon source; disodium hydrogen phosphate and potassium dihydrogen phosphate as a buffer; ox bile and brilliant green as selective antibacterial agent, inhibiting the growth of non-Enterobacteriaceae.
Ingredients | /liter |
Pancreatic digest of gelatin | 10g |
Glucose monohydrate | 5g |
Dehydrated ox bile | 20g |
Potassium dihydrogen phosphate | 2g |
Disodium hydrogen phosphate dihydrate | 8g |
Brilliant green | 15mg |
pH7.2±0.2 at 25°C |
Suspend 45g in 1L of distilled water,stir until completely dissolved and dispense 100 mL into test tubes , heat at 100 °C for 30 min in a waterbath or flowing steam.
The following quality control strains were inoculated and cultured at 30-35℃ for 24h-48h. The results are as follows:
Quality control strains | Inoculum (CFU) | Growth |
Escherichia coli ATCC8739 | < 100 | Good growth |
Pseudomonas aeruginosa ATCC9027 | ||
Staphylococcus aureus ATCC6538 | > 100 | Inhibited |
2-30℃,Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Microbiological contamination was disposed by autoclaving at 121°C for
30 minutes.
Intended Use For enriching bile-tolerant Gram-negative bacteria in the microbiological examination of pharmaceutical products. Principle and Interpretation Pancreatic digest of gelatin pro……
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