Plasmid Purification Magnetic Beads (RNA Depletion)

Plasmid isolation from bacterial cultures is one of the most popular techniques in biomedical research and pharmaceutical industries. However, it is common that the isolated plasmid DNA is usually contaminated with varied degrees of host RNA. Plasmid purification is necessary to reduce the impact on downstream applications by removing RNA contamination.

We have developed a simple reagent to completely remove RNA contamination in the isolated plasmid samples using Solid Phase Reversible Immobilization (SPRI) beads. SPRI beads consist of paramagnetic particles coated with carboxyl groups that reversibly bind DNA. Our Plasmid Purification Magnetic Beads (RNA Depletion) combines BioDynami’s proprietary chemistries with the reversible DNA-binding properties of SPRI magnetic beads. The reagent removes RNA and recovers the plasmid in the same step.  Moreover, unwanted components such as salts, dNTPs, proteins, enzymes, and other impurities can also be removed simultaneously.

Plasmid can be used for downstream applications such as enzymatic digestion, transformation, transfection and molecular cloning etc.  The beads can be an effective and inexpensive reagent for bacterial RNA depletion for routine plasmid purification.

Features

• Effective depletion of bacterial RNA by RNase
• High recovery rate of plasmid DNA by magnetic beads
• Removal of unwanted components and impurities
• Simple and fast beads-based protocol

Plasmid isolation from bacterial cultures is one of the most popular techniques in biomedical research and pharmaceutical industries. However, it is common that the isolated plasmid DNA is usually contaminated with varied degrees of host RNA. Plasmid purification is necessary to reduce the impact on downstream applications by removing RNA contamination.

K-RAFGL

SKU: 700004332

120 assays of each per kit

The Raffinose/Sucrose/D-Glucose test kit is for the measurement and analysis of D-glucose, sucrose and raffinose, stachyose and verbascose in seeds and seed meals. Based on the measurement of D-glucose on enzymic hydrolysis of raffinose, stachyose and verbascose to D-glucose, D-fructose and D-galactose.

Check out our full range of monosaccharide and oligosaccharide assay kits.

Advantages

• Very competitive price (cost per test) 
• All reagents stable for > 2 years after preparation 
• Simple format 
• Rapid reaction 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included

The Raffinose/Sucrose/D-Glucose test kit is for the measurement and analysis of D-glucose, sucrose and raffinose, stachyose and verbascose in seeds and seed meals. Based on the measurement of D-glucose on enzymic hydrolysis of raffinose, stachyose and verbascose to D-glucose, D-fructose and D-galactose.

Real-time fluorescent DNA amplification made possible with a TwistAmp® exo kit. Recommended for users who want to combine TwistDx’s RPA amplification technology with the use of TwistDx’s proprietary fluorescent TwistAmp® exo probe in a homogenous format. The user need only supply primers, probe, and template. See manual for more information. Click to order oligonucleotides.

Real-time fluorescent DNA amplification made possible with a TwistAmp® exo kit. Recommended for users who want to combine TwistDx’s RPA amplification technology with the use of TwistDx’s proprietary fluorescent TwistAmp® exo probe in a homogenous format. The user need only supply primers, probe, and template. See manual for more information. Click to order oligonucleotides.