This product is suitable for extracting total pathogen RNA from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified RNA can be used for clinical in vitro detection.

It is special designed for Pathogen RNA extraction from Sputum samples, can be used in tuberculosis (TB) detection.

Details

Specifications

Features	Specifications
Main Function	Extract total pathogen RNA from cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant, special design for sputum samples. Used in tuberculosis detection.
Applications	RT-PCR，PCR
Products	Pathogen RNA
Purification method	Polydisperse magnetic beads
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant, sputum
Sample amount	200-300μl
Adaptive instrument	Nucleic acid extractor, pipetting workstation

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.

Advantages

• Fast – several samples can be extracted in 40 minutes by column method
• High quality – high purity total RNA / DNA can be directly used in various sensitive downstream applications
• Safe – no phenol chloroform extraction required
• Sensitive – DNA/RNA can be recovered at the level of PG

Kit Contents

Contents	IVD6672C
Purification Times	200 Preps
2ml Bead Tube	4 x 50
MagPure Particle	7.0 ml
Proteinase K	100 mg
Protease Dissolve Buffer	6 ml
DTT （Powder)	2g
Buffer SDS	15 ml
Buffer MLBN	220 ml
DNase I	4 x 0.6 ml
DNase Buffer	60 ml
Buffer MW1*	53 ml
Buffer MW2*	50 ml
Buffer NFW	30 ml

Storage and Stability

MagPure Particles and Proteinase K, DNase I  should be stored at 2–8°C upon arrival. However, short-term storage (up to 2 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.

This product is suitable for extracting total pathogen RNA from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified RNA can be used for clinical in vitro detection.

Overview

• Detection kits for HEV
• CE-IVD marked version available for in vitro diagnostic use
• Available in TaqMan format for analysis

The Enteroviruses are a genus of the family Picornaviridae, and are a large and diverse group of small RNA viruses characterized by a ssRNA molecule of positive sense. To date, over 70 serotypes of Enterovirus have been isolated from man. The human enteroviruses include poliovirus, Coxsackie A and Coxsackie B viruses, enterocytopathic human orphan (ECHO) viruses and other enteroviruses. Enteroviruses infect millions of people worldwide each year, and the infection can result in a wide variety of symptoms depending on the serotype of the enterovirus. Symptoms of infection may include, but are not limited to, mild respiratory illness (common cold), hand, foot and mouth disease, Rubelliform rashes, acute hemorrhagic conjunctivitis, aseptic meningitis, myocarditis, and neonatal infections.

HEV TaqMan RT-PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

HEV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM39750 (100 preps)	Cat. TM39710 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
Enterovirus Primer & Probe Mix	280 μL	280 μL
Enterovirus Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Biotin-PEG2-alkyne is a short PEG linker featuring a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays while terminal alkynes are used in copper (I) click chemistry with azides.

Biotin-PEG2-alkyne is a short PEG linker featuring a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays while terminal alkynes are used in copper (I) click chemistry with azides.