Propargyl-PEG4-acid is a PEG reagent with an alkyne and a terminal carboxylic acid. The carboxylic acid reacts with primary amine groups with the help of activators (e.g. EDC, or HATU). The 4-unit PEG spacer increases hydrophilicity. The alkyne can participate in copper-catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG4-acid is a PEG reagent with an alkyne and a terminal carboxylic acid. The carboxylic acid reacts with primary amine groups with the help of activators (e.g. EDC, or HATU). The 4-unit PEG spacer increases hydrophilicity. The alkyne can participate in copper-catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Hipure Stool RNA Kit is specially designed for stool RNA extraction. This kit is suitable for extracting high-purity microbial or host cell RNA from ≤0.1g stool samples. The kit adopts silica gel column purification technology and original solution system, which can effectively remove humic acid and other inhibitory factors in stool samples. The purified RNA can be directly used in RT-PCR, Northern hybridization and other experiments.
Specifications
Features | Specifications |
Main Functions | Isolation total RNA from 100-150mg stool sample |
Applications | RT-PCR, Northern hybridization and other experiments |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Stool |
Sample amount | 100-150 mg |
Elution volume | ≥30μl |
Time per run | ≤50 minutes |
Liquid carrying volume per column | 100µg |
Binding yield of column | 800µl |
The HiPure silica gel column uses a high binding ability glass fiber filter membrane as the substrate. Under the condition of high concentration of ionizing agent (such as Guanidinium chloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bonding and electrostatic and other physical factors, while protein or other impurities are not adsorbed and removed. The filter membrane that has adsorbed nucleic acids is washed to remove proteins and salts. Finally, low salt buffer solution (such as Buffer TE) or water can be used to wash out the nucleic acids adsorbed on the filter membrane. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
The stool samples are homogenized in the lysis solution, further lysed in a high-temperature water bath, and RNA is released into the lysis solution. Chloroform extraction removes genomic DNA and impurities, transfer the supernatant to an alcohol free binding solution, purify RNA through a column, and finally elute RNA with RNase Free Water. The purified RNA can be directly used for experiments such as PCR, Southern hybridization, and enzyme digestion.
Advantages
Kit Contents
Contents | R418502 | R418503 |
Purification Times | 50 Preps | 250 Preps |
HiPure RNA Mini Columns | 50 | 250 |
2ml Collection Tubes | 50 | 250 |
Glass Beads (0.1~0.6mm) | 30 g | 150 g |
Buffer SPL | 30 ml | 140 ml |
Buffer PHC | 30 ml | 140 ml |
Buffer GRP | 60 ml | 250 ml |
Buffer RW1 | 50 ml | 250 ml |
Buffer RW2 * | 20 ml | 2 x 50 ml |
RNase Free Water | 15 ml | 30 ml |
Storage and Stability
The kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions. At low temperatures, Buffer SPL may form precipitates, dissolve it by 55°C water bath. After receiving the product, Buffer PHC should be stored at 2-8°C.
Hipure Stool RNA Kit is specially designed for stool RNA extraction. This kit is suitable for extracting high-purity microbial or host cell RNA from ≤0.1g stool samples. The kit adopts silica gel column purification technology and original solution system, which can effectively remove humic acid and other inhibitory factors in stool samples. The purified RNA can be directly used in RT-PCR, Northern hybridization and other experiments.
These kits are suitable for the isolation of total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. These kits purify all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
Total RNA Purification 96-Well Kit (High Throughput and High Throughput Deep Well)
This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.
Isolate RNA after Purifying EVs and Exosomes
Ultracentrifugation, Exoquick, Filtration
Cat. # | Name | Elution Volume | Plasma/Serum | Urine | Cell-Culture Media |
---|---|---|---|---|---|
55000 | Plasma/Serum RNA Purification Mini Kit | 10 – 25 µL | 50 µL – 1 mL | 250 µL – 1 mL | 5 – 10 mL |
35300 | Total RNA Purification Micro Kit | 20 – 50 µL | 1 – 4 mL | 2 – 10 mL | 10 – 20 mL |
17200 | Total RNA Purification Kit | 50 – 100 µL | 4 – 10 mL | 11 – 30 mL | 20 – 35 mL |
Figure 1 / 13
Click for expanded view
Kit Specifications | |
Maximum Binding Capacity | Up to 50 μg RNA |
Maximum Loading Volume | 650 μL |
Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
Maximum Amount of Starting Material | |
Animal Cells | 3 x 106 cells |
Animal Tissues | 10 mg (for most tissues*) |
Blood | 100 μL |
Plasma/Serum | 200 μL |
Bacteria | 1 x 109 cells |
Yeast | 1 x 108 cells |
Fungi | 50 mg |
Plant Tissues | 50 mg |
Time to Complete 10 Purifications | 20 minutes |
Average Yield | |
HeLa Cells (1 x 106 cells) | 15 μg |
E. coli (1 x 109 cells) | 50 μg |
* for isolating total RNA from larger amounts of tissue, please use Norgen’s Animal Tissue RNA Purification Kit (Cat# 25700)
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.
Component | Cat. 17200 (50 preps) | Cat. 37500 (100 preps) | Cat. 17250 (250 preps) | Cat. 17270 (500 preps) | Cat. 24300 (192 preps) | Cat. 24370 (576 preps) | Cat. 24350 (192 preps) | Cat. 24380 (576 preps) |
---|---|---|---|---|---|---|---|---|
Buffer RL | 40 mL | 2 x 40 mL | 175 mL | 350 mL | 2 x 40 mL | 350 mL | 2 x 40 mL | 350 mL |
Wash Solution A | 38 mL | 2 x 38 mL | 148 mL | 1 x 148 mL 1 x 74 mL | 2 x 38 mL | 1 x 74 mL 1 x 148 mL | 2 x 38 mL | 1 x 74 mL 1 x 148 mL |
Elution Solution A | 6 mL | 2 x 6 mL | 30 mL | 60 mL | 2 x 20 mL | 60 mL | 2 x 20 mL | 60 mL |
Mini Spin Columns | 50 | 100 | 250 | 500 | – | – | – | – |
96-Well Isolation Plate | – | – | – | – | 2 | 6 | – | – |
96-Well Isolation Plate (Deep Well) | – | – | – | – | – | – | 2 | 6 |
Adhesive Tape | – | – | – | – | 4 | 12 | 4 | 12 |
Collection Tubes | 50 | 100 | 250 | 500 | – | – | ||
96-Well Collection Plate | – | – | – | – | 2 | 6 | – | – |
96-Well Collection Plate (Deep Well) | – | – | – | – | – | – | 2 | 6 |
Elution Tubes (1.7 mL) | 50 | 100 | 250 | 500 | – | – | ||
96-Well Elution Plate | – | – | – | – | 2 | 6 | – | – |
96-Well Elution Plate (Deep Well) | – | – | – | – | – | – | 2 | 6 |
Product Insert | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
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