This product is suitable for rapid extraction of RNA from low RNA yield somples such as tissue (<10mg), cells, bone marrow, fresh blood, and other clinical samples. RNA can be used directly for RT-PCR, Real time PCR, NGS, Viral RNA detection and so on.
Specifications
Features | Specifications |
Main Functions | Isolation total RNA from tissue, cell, whole blood |
Applications | RT-PCR, cDNA synthesis, second generation sequencing |
Purification method | Polydisperse magnetic beads |
Purification technology | Magnetic beads technology |
Process method | Manual or automatic |
Adaptive instrument | Nucleic acid extractor, pipetting workstation |
Sample type | Tissues, cells, lymphocytes and other clinical sample |
Sample amount | Cells grown in suspension: ≤3 x 106Animal tissue: ≤10mgPlant tissue: ≤30mgWhole blood: 0.5~1.0 ml fresh blood or bone marrow and fresh blood mixture |
Kit Contents
Contents | IVD3020B-96 | IVD3020B |
Purification Times | 96 Preps | 200 Preps |
MagPure Particles N | 2.5 ml | 5 ml |
DNase I | 2 x 600 µl | 4 x 600 µl |
DNase Buffer C | 60 ml | 120 ml |
Buffer RLC | 60 ml | 120 ml |
Buffer MCB* | 60 ml | 150 ml |
Buffer GW1* | 44 ml | 110 ml |
Buffer MW2* | 50 ml | 100 ml |
RNase Free Water | 20 ml | 60 ml |
Storage and Stability
DNase I should be shipped with ice pack and stored at -20°C after arrival. MagPure Particles N should be stored at 2–8°C for long time storage. The remaining kit components can be stored at room temperature(15–25°C) for up to18 months under these conditions.
This product is suitable for rapid extraction of RNA from low RNA yield somples such as tissue (<10mg), cells, bone marrow, fresh blood, and other clinical samples. RNA can be used directly for RT-PCR, Real time PCR, NGS, Viral RNA detection and so on.
West Nile Virus (WNV) belongs to the RNA virus family of Flaviviridae. It is known to spread primarily through arthropod vectors such as mosquitoes. The virus infects mainly birds but is also reported to infect humans as well as pets such as cats and dogs. In humans, the majority of the infections caused by WNV are asymptomatic. However, some individuals (the majority of the confirmed cases) could enter a second, febrile stage with flu-like symptoms – commonly known as West Nile Fever. In a more serious stage, the disease becomes neuroinvasive, causing meningitis or encephalitis. Such severe conditions could lead to mortality. As the symptoms of WNV are very similar to other common diseases but can be fatal at a severe stage, it is important to distinguish the virus early in the diagnosis, particularly at the molecular level.
WNV TaqMan RT-PCR Kit, 100 reactions
WNV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Figure 1 / 3
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Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component | Cat. TM44250 (100 preps) | Cat. TM44210 (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
WNV Primer & Probe Mix | 280 μL | 280 μL |
WNV Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |
The CloneSizer 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our CloneSizer contains fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments, double intensity reference bands at 500 and 1000 bp and an additional 2686 bp (pUC19) reference band for easy clone identification.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Figure 1 / 1
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CloneSizer 100 bp DNA Ladder (Cat# 11600) – 100 loads
Ladder Properties:
Fragment | Size (bp) | Mass (ng) |
1 | 2686 | 72 |
2 | 2000 | 53 |
3 | 1500 | 41 |
4 | 1200 | 42 |
5 | 1000 | 56 |
6 | 900 | 30 |
7 | 800 | 29 |
8 | 700 | 25 |
9 | 600 | 25 |
10 | 500 | 52 |
11 | 400 | 19 |
12 | 300 | 20 |
13 | 200 | 19 |
14 | 100 | 17 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
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