Primer and probe mix (150 reactions) Reverse Transcription, target specific primers (RNA genome viruses only) Copy number standard curve (sufficient for multiple standard curves) Internal extraction control – Read through VIC channel* Endogenous control (150 tests) RNAse/DNAse free water *alternative fluorophores available on request
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp (DNA). HiPure Circulating DNA Mini Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. Samples can be fresh or frozen (provided that they have not been frozen and thawed more than once).
Details
Specifications
Features
Specifications
Main Functions
Isolation circulating DNA from 1ml plasma, serum, body fluids
Applications
qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Serum, plasma and other cell-free fluid samples
Sample amount
1ml
Elution volume
≥30μl
Time per run
≤50 minutes
Liquid carrying volume per column
800µl
Binding yield of column
100µg
Principle
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption plate and filter column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10 Mm Tris,pH 8.0).
Advantages
High yield – most optimal process, free DNA (>50bp) can be obtained to the maximum extent
High concentration – low elution volume, ensuring high nucleic acid concentration
High purity – low alcohol binding method, completely removing inhibitor and protein pollution
High recovery – DNA can be recovered at the level of PG by silica gel column purification
Kit Contents
Contents
D318102
D318103
Purification Times
50 Preps
250 Preps
Buffer ACL
50 ml
250 ml
Buffer ACB*
60 ml
300 ml
Buffer DCW1*
22 ml
88 ml
Buffer DCW2*
10 ml
50 ml
Proteinase K
120 mg
540 mg
Protease Dissolve Buffer
10 ml
30 ml
Carrier RNA
110 μg
310 μg
Nuclease Free Water
10 ml
30 ml
HiPure CFDNA Mini Columns
50
250
2 ml Collection Tubes
100
500
Storage and Stability
Proteinase K and carrier RNA should be stored at 2–8°C upon arrival. However, short-term storage(up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remainingkit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers shouldbe redissolved before use. Make sure that all buffers are at room temperature when used.
Document
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments,
One cDNA Synthesis, Multiple microRNAs and microRNA-targets analyzed
Time Savings
Cost Efficient
High Sensitivity and Yield
Robust Enzyme
Available in 12 or 50 reaction size
Norgen’s microScript microRNA cDNA Synthesis Kit is an all-in-one, ready-to-use product for the reverse transcription of microRNA from either Total RNA preparations or enriched microRNA preparations. The kit contains the 2x Reaction Mix and the microScript microRNA Enzyme Mix. The kit utilizes Norgen’s microScript Reverse Transcriptase, a mutant version of Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase. It has reduced RNase H activity and increased thermal stability.
The workflow of Norgen’s microScript microRNA cDNA Synthesis Kit involves a simple, single-tube set-up by the mixing of 2x Reaction Mix, Enzyme Mix and the RNA template. The reaction can then be carried out in a thermocycler. A poly (A) tail is first added to the RNA template, followed by cDNA synthesis using an adapter primer. In addition to the ease-of-use, the single-tube set-up provides superb consistency and sensitivity. The cDNA could be used in a PCR or qPCR amplification using a Universal PCR Reverse Primer and the forward primer that contains the sequence of the microRNA of interest. A single cDNA preparation could be used for PCR amplification of a number of different microRNAs. In addition, the cDNA preparation could be used for PCR or qPCR detection (using gene-specific forward and reverse primers) of mRNA or large RNA if total RNA preparation was the starting template. This could allow for parallel evaluation of expression level of microRNAs and microRNA-targets.
