Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
K-MALTA
SKU: 700004315
100 assays (50 of each) per kit
| Content: | 100 assays (50 of each) per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | α-Amylase, β-Amylase |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 400 |
| Signal Response: | Increase |
| Limit of Detection: | 0.05 U/mL |
| Reaction Time (min): | ~ 20 min (Ceralpha Method), ~ 10 min (Betamyl-3 Method) |
| Application examples: | Cereal flours, malts, fermentation broths and other materials. |
| Method recognition: | “Ceralpha” Method: AACC Method 22-02.01, AOAC Method 2002.01, ICC Standard No. 303, RACI Standard Method and CCFRA (Flour Testing Working Group Method 0018). “Betamyl-3” Method: RACI Standard Method |
The Malt Amylase test kit is suitable for the specific measurement and analysis of α-amylase and of β-amylase in malt flour.
Other enzyme activity test kits available.
Advantages
The Malt Amylase test kit is suitable for the specific measurement and analysis of α-amylase and of β-amylase in malt flour.
HiPure PCR Pure Mini Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 70bp-30kbp with yields exceeding 85%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Compared with the gel purification kit, this product uses guanidine hydrochloride mediated adsorption, which have better A260/A230 ratio.
Specifications
| Features | Specifications |
| Main Functions | Recover DNA fragments >100bp from various pcr products, enzymatic reaction solutions, labeling reaction solutions, crude DNA, etc. |
| Applications | PCR, sequencing, labeling reactions, ligations and restriction digestion, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | PCR products, enzymatic reaction solution |
| Sample amount | 10-200μl |
| Recovery | ≥85% |
| Elution volume | ≥15μl |
| Time per run | ≤10 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 35µg |
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
Kit Contents
| Contents | D212102 | D212103 |
| Purification Times | 100 Preps | 250 Preps |
| Buffer DP | 60 ml | 120 ml |
| Buffer DW2 | 20 ml | 50 ml |
| Elution Buffer | 15 ml | 30 ml |
| HiPure DNA Mini Columns II | 100 | 250 |
| 2 ml Collection Tubes | 100 | 250 |
Storage and Stability
The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
Experiment Data
HiPure PCR Pure Mini Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 70bp-30kbp with yields exceeding 85%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Compared with the gel purification kit, this product uses guanidine hydrochloride mediated adsorption, which have better A260/A230 ratio.