Attogene Universal Lateral Flow Assay Kits are a convenient ready-to-use kit for quick and cost-effective development of a lateral flow dipstick assay for detection of DNA and RNA products.
Formats (strep gold conjugate pad):
Detection of nucleic Acid (DNA or RNA) requires the use of a biotin and FAM-labelled primer during amplification. Test line: anti-FITC/FAM, Control Line: Biotin
Multiplex detection of nucleic Acid (DNA or RNA) requires the use of a biotin, FITC/FAM and Dig labelled primers during amplification.: Test Line #1: anti FITC/FAM, Line #2: anti-Dig, Line #3 Biotin.
Inquire about custom configurations: [email protected]
Kit Components
Features & Benefits
50 Lateral Flow Dipsticks (4.5mm)
10 mL Sample assay running buffer
96 well plate
Controls
Norgen’s Total RNA Purification Kits Dx provide rapid methods for the isolation and purification of total RNA from tissue samples, blood, plasma, serum, saliva, bacteria, yeast, fungi and viruses for subsequent in vitro diagnostic use. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
Total RNA Purification Kit Dx (Spin Column)
This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Norgen’s Total RNA Purification Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.
Total RNA Purification 96-Well Kits Dx (High Throughput and High Throughput Deep Well)
Thess 96-well kits provide a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.
Intended Use
To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used. Norgen’s Total RNA Purification Kits Dx are intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques. Norgen’s Total RNA Purification Kits Dx do not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.
NOTE: This product is not available for sale in the United States.
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| Kit Specifications | |
| Maximum Binding Capacity Per Well | 50 μg |
| Maximum Loading Volume Per Well | 500 μL |
| Size of RNA Purified | All sizes, including small RNA < 200 nt |
| Time to Complete 96 Purifications | 30 minutes |
| Average Yield HeLa Cells (1 x 106 Cells) E. coli (1 x 109 cells) Brain (10 mg) Liver (10 mg) Blood (50 μL, Hamster) | 15 μg 50 μg 9 μg 26 μg 1 μg |
| Maximum Amount of Starting Material: Whole Blood Plasma/Serum Bacteria Yeast Fungi Plant Tissue | 100 µL150 µL1 x 109 cells1 x 108 cells 40 mg 40 mg |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
| Component | Cat. Dx17200 (50 preps) | Cat. Dx24300 (192 preps) | Cat. Dx24350 (192 preps) | Cat. Dx24380 (576 preps) |
|---|---|---|---|---|
| Lysis Solution | 40 mL | – | – | – |
| Buffer RL | – | 2 x 40 mL | 2 x 40 mL | 350 mL |
| Wash Solution | 22 mL | – | – | – |
| Wash Solution A | 22 mL | 2 x 38 mL | 2 x 38 mL | 1 x 148 mL 1 x 74 mL |
| Elution Solution | 6 mL | – | – | – |
| Elution Solution A | – | 2 x 20 mL | 2 x 20 mL | 60 mL |
| Mini Spin Columns | 50 | – | – | – |
| 96-Well Isolation Plate | – | 2 | 2 | – |
| 96-Well Isolation Plate (Deep Well) | – | – | – | 6 |
| Collection Tubes | 50 | – | – | – |
| 96-Well Collection Plate | – | 2 | 2 | – |
| 96-Well Collection Plate (Deep Well) | – | – | – | 6 |
| Elution Tubes (1.7 mL) | 50 | – | – | – |
| 96-Well Elution Plate | – | 2 | 2 | – |
| 96-Well Elution Plate (Deep Well) | – | – | – | 6 |
| Adhesive Tape | – | 4 | 4 | 12 |
| Product Insert | 1 | 1 | 1 | 1 |
E. coli O157:H7 is a rod-shaped, gram negative bacterium. It is an enterohemorrhagic strain of the common E. coli bacterium and infection by the O157:H7 strain is commonly associated with hemorrhagic colitis. E. coli O157:H7 is recognized by its somatic (cell wall) antigen (O157) and its flagella antigen (H7). In addition, E. coli O157:H7 is known to produce Shiga-like toxins, which cause severe symptoms. While most patients can recover from the infection, up to 15% of the patients may develop hemolytic uremic syndrome, a type of kidney failure that could be fatal. Infection of E. coli O157:H7 usually results from consumption of poorly prepared food including undercooked meat (particularly ground beef), untreated water or raw unpasteurized milk.
Norgen’s E. coli O157:H7 Quantified Bacterial DNA Standards are cloned fragments of the two Shiga-like toxin regions purified using Norgen’s sample preparation technology. They include partial sequences of STX-1 subunit A gene and STX-2 subunit A gene. Please refer to Appendix A for sequence information. The purified DNA is quantified vigorously using multiple methods including spectrophotometry, gel densitometry and real-time PCR. It is intended to be used as positive controls or PCR quantification standards for E. coli O157:H7. This product is compatible with Norgen Biotek’s E. coli O157:H7 Taqman PCR Detection Kit (Cat. TM41350) and E. coli O157:H7 Taqman PCR Detection Probe/Primer and Control Set (Cat. TM41310).
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| Volume Provided | 250 μL (for STX1)250 μL (for STX2) |
| DNA Quantity | 2 x 104 copies per μL |
Storage Conditions
Upon receipt, store Norgen’s E. coli O157:H7 Quantified Bacterial DNA Standards at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.