MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.
This method is suitable for small quantities of tissue (<100mg) and cells (<5 X106), and large quantities of tissue (up to 1g) and cells (<108), of human, animal, plant, or bacterial origin. The simplicity of the MagZol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour. Total RNA prepared in this manner can be used for Northern blot analysis, dot blot hybridization, poly(A) + selection, in vitro translation, RNase protection assay, and molecular cloning. For use in amplification by thermal cycling, treatment of the isolated RNA with RNase-free DNase I is recommended when the two amplimers lie within a single exon.
Specifications
| Features | Specifications |
| Main Functions | Extract RNA from liquid samples by salting out method |
| Applications | RT-PCR, Northern hybridization, poly (a) enrichment, etc. |
| Purification technology | Acid phenol guanidine isothiocyanate |
| Process method | Manual (centrifugation) |
| Sample type | Various liquid samples |
| Sample amount | Flexible |
| Elution volume | Variation with sample size |
| Time per run | Variation with sample size |
Storage and Stability
MagZol Reagent should be stored at 2-8°C upon arrival and is stable for at least 24 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance.
MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.
endo-BCN-PEG2-Acrylate is a PEG linker containing a BCN group and an acrylate group. The BCN group enables copper free click chemitry with azide-tagged molecules. The acrylate group enables Michael addition reactions. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research use only.
endo-BCN-PEG2-Acrylate is a PEG linker containing a BCN group and an acrylate group. The BCN group enables copper free click chemitry with azide-tagged molecules. The acrylate group enables Michael addition reactions. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research use only.
K-ACHDF
SKU: 700004259
100 assays of each component
| Content: | 100 assays of each component |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Available Carbohydrates, Dietary Fiber |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Limit of Detection: | 0.5 g/100 g |
| Reaction Time (min): | ~ 78 min |
| Application examples: | Food ingredients, food products and other materials. |
The Available Carbohydrates/Dietary Fiber test kit is an integrated procedure for the measurement and analysis of available carbohydrates and dietary fiber in cereal products, fruit and vegetables and food products.
See more of our dietary fiber products.
Advantages
The Available Carbohydrates/Dietary Fiber test kit is an integrated procedure for the measurement and analysis of available carbohydrates and dietary fiber in cereal products, fruit and vegetables and food products.