E. coli O157:H7 is a rod-shaped, gram negative bacterium. It is an enterohemorrhagic strain of the common E. coli bacterium and infection by the O157:H7 strain is commonly associated with hemorrhagic colitis. E. coli O157:H7 is recognized by its somatic (cell wall) antigen (O157) and its flagella antigen (H7). In addition, E. coli O157:H7 is known to produce Shiga-like toxins, which cause severe symptoms. While most patients can recover from the infection, up to 15% of the patients may develop hemolytic uremic syndrome, a type of kidney failure that could be fatal. Infection of E. coli O157:H7 usually results from consumption of poorly prepared food including undercooked meat (particularly ground beef), untreated water or raw unpasteurized milk.
E.coli O157:H7 TaqMan PCR Kit, 100 reactions
E.coli O157:H7 TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component | Cat. TM41350 (100 preps) | Cat. TM41310 (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
E. coli O157:H7 Primer & Probe Mix | 280 μL | 280 μL |
E. coli O157:H7 Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |
DBCO-PEG2-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde), etc. The hydrophilic PEG spacer increases the water solubility of the compound. Reagent grade, for research purpose.
DBCO-PEG2-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde), etc. The hydrophilic PEG spacer increases the water solubility of the compound. Reagent grade, for research purpose.
The FastRunner DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains eight discrete fragments ranging from 50 bp to 2000 bp with a higher intensity reference band at 500 bp. This Ladder is ideal for quick sizing of PCR products and restriction digests.
Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
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FastRunner DNA Ladder (Cat# 12800) – 100 loads
Ladder Properties:
• Eight discrete bands, ranging from 50 bp to 2000 bp
• Higher intensity band at 500 bp for easy reference
Fragment | Size (bp) | Mass (ng) |
1 | 2000 | 104 |
2 | 1500 | 88 |
3 | 1000 | 68 |
4 | 750 | 59 |
5 | 500 | 93 |
6 | 300 | 28 |
7 | 150 | 35 |
8 | 50 | 25 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
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