4×7×50ml Swing Rotor 

Overview

• Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more
• Simple and quick workflow: library could be prepared in less than 5 hours
• Component of Norgen’s metagenomics workflow
• A single NGS run can be prepared with up to 384 unique dual-index libraries

Sample type purification kit guide 

The 16S V2-V3 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V2-V3 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.

The 16S V2-V3 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V2-V3 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.

Details

Supporting Data

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Minimum amount of starting material:	2.5 µL of DNA (5 ng/µL)
Time to complete library preparation:	4 hours

Storage Conditions and Product Stability
Norgen’s 16S V2-V3 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components should remain stable for at least 1 year when stored at the specified storage conditions.

Step	Component	Cat. 70300 (24 preps)	Cat. 70310, 70320, 70330, 70340 (96 preps)
Amplicon PCR (PCR 1)	MGX Master Mix	330 µL	1,320 µL
	16S V2-V3 Primer Mix	70 µL	280 µL
Index PCR (PCR 2)	Indexing Master Mix	660 µL	2 x 1,320 µL
	N7xx Index Primer	50 µL	50 µL
	S5xx Index Primer	70 µL	70 µL
PCR Clean-Up	Resuspension Buffer	2 x 1,250 µL	2 x 5,000 µL
	Nuclease-free water	1,250 µL	1 x 6,000 µL

 Features 

1.Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.  

2.Imported compressors fluorine free,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control.

3.Microprocessor control, digital display which indicates the temperature, speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.

4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.

5. Automatically electric lid lock, over speed, over temperature protection and imbalance protection.

6.High quality steel centrifuge body , stainless steel chamber, built-in stainless steel explosion-proof protect sleeve , 3 tiers protection,safe and reliable.

7. Rotor is connected to spindle by specialized spring taper sleeve, loading simple and quick, no direction

8. With pre-cooling and get the ideal centrifugation result.

 TGL16 Technical Parameter：

Max. Speed	16000rpm
Max. RCF	20600×g
Max. Capacity	6×100ml
Time Range	1~9h59min
RPM/RCF Convert	Yes
Noise (dB)	≤ 58
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	±1℃
Voltage(V/Hz)	AC 220V/110V  50HZ/60HZ
Size (L x W x Hmm)	610×570×370mm
Net Weight(Kg)	82KG
Certificates	TUV CE,ISO 9001,ISO13485 & Calibration report are available

 Matched Rotor for TGL16 

Order No	Rotor	Max speed (rpm)	Max Volume(ml)	Max RCF (g)
G16-1	Angle rotor	14000	4×8PCR	12070
G16-2	Angle rotor	13000	6×8PCR	16080
G16-3	Angle rotor	14000	8×8PCR	13390
G16-4	Angle rotor	13000	12×8PCR	17220
G16-5	Angle Rotor	15000	30×0.5ml	18510
G16-6	Angle Rotor	14000	40×0.5ml	19970
G16-7	Angle Rotor	16000	12×1.5/2ml	17940
G16-8	Angle Rotor	15000	24×1.5/2ml	20600
G16-9	Angle Rotor	13500	30×1.5/2ml	19340
G16-10	Angle Rotor	11000	48×1.5/2ml	12840
G16-11	Angle Rotor	15000	16×5ml	19350
G16-12	Angle Rotor	14000	12×7ml	16370
G16-13	Angle Rotor	14000	6×10ml	16460
G16-14	Angle Rotor	12000	12×10ml	14510
G16-15	Angle Rotor	10000	12×15ml	11840
G16-16	Angle Rotor	5000	24×15ml	3080
G16-17	Angle Rotor	5000	30×15ml	3830
G16-18	Angle Rotor	12000	8×20ml	14510
G16-19	Angle Rotor	12000	6×30ml	14000
G16-20	Angle Rotor	11000	6×50ml	13480
G16-21	Angle Rotor	12000	6×70ml	10810
G16-22	Angle Rotor	8000	6×100ml	7280
G16-23	Angle Rotor	12000	24 pieces capillary vessel	15800
G16-24	Microplate rotor	4000	2×3×48 Well	2300

The Opentrons Flex 96-Channel pipette enables accurate high throughput pipetting, optimized for working in 96-well plates. Flex Pipettes use air displacement technology to offer highly accurate pipetting with pipette volume ranges from 5 – 1000 μL.

Smart sensors support automatic calibration, real-time positioning, and error detection. The 96-channel pipette occupies both pipette mounts and can easily be removed and swapped to an alternate pipette configurations to adapt your workflow needs.

Four 96-Channel Tip Rack Adapters are included with the purchase of one Flex 96-Channel Pipette.

The Opentrons Flex 96-Channel pipette enables accurate high throughput pipetting, optimized for working in 96-well plates. Flex Pipettes use air displacement technology to offer highly accurate pipetting with pipette volume ranges from 5 – 1000 μL.

Smart sensors support automatic calibration, real-time positioning, and error detection. The 96-channel pipette occupies both pipette mounts and can easily be removed and swapped to an alternate pipette configurations to adapt your workflow needs.

Four 96-Channel Tip Rack Adapters are included with the purchase of one Flex 96-Channel Pipette.