6x1000ml Swing Rotor 

Overview

• Detection kits for the HPV (High Risk)
• Available in TaqMan format for analysis

More than 70 types of human papillomavirus (HPV) have been identified, and are generally classified as high-risk or low-risk depending on their relationship or lack of relationship with cancer and high-grade cervical intraepithelial neoplasia (CIN 2-3). HPV viruses are predominantly sexually transmitted and high-risk HPV types are a major risk factor for development of cervical cancer. Low-risk HPV types 6 and 11 have been associated with the presence of genital warts. There are many other low-risk HPV types that are not associated with genital warts or cervical cancer. Until now, HPV cannot be cultured in vitro, and immunological tests are inadequate to determine the presence of HPV cervical infection. On the other hand, biopsies can be analyzed by nucleic acid hybridization to directly detect the presence of HPV DNA. HPV 16 and HPV 18 have been considered as high-risk cancer associated HPV types.

HPV (High Risk) TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

HPV (High Risk) TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Component	Cat. TM32250 (100 preps)	Cat. TM32210 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
HPV (High Risk) Primer & Probe Mix	280 μL	280 μL
HPV (High Risk) Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

K-AMYL

SKU: 700004262

100 assays per kit

Content:	100 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Amylopectin, Amylose
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	510
Signal Response:	Increase
Limit of Detection:	Amylose 5-95% of total starch content
Total Assay Time:	~ 120 min
Application examples:	Cereal starches, flours, pure starches and foods.
Method recognition:	Novel method

The Amylose/Amylopectin test kit is suitable for the measurement and analysis of amylose/amylopectin ratio and content in cereal starches and flours. Based on a Con A precipitation procedure.

See our full list of polysaccharide test kits.

Advantages

• Very cost effective (cost per test) 
• All reagents stable for > 12 months after preparation 
• Only enzymatic kit available 
• Accurate and reliable amylose / amylopectin ratio determination  
• Simple format 
• Standard included

The Amylose/Amylopectin test kit is suitable for the measurement and analysis of amylose/amylopectin ratio and content in cereal starches and flours. Based on a Con A precipitation procedure.

• Format: 96-well microtiter plate (12 test strips of 8 wells)
• Standards: 0 | 0.03 | 0.10 | 0.2 | 2 ppb
• Incubation Time: 45 Minutes
• Compatible for use with US EPA Method 546

Description

The Cylindrospermopsin plate kit is a competitive enzyme-labeled immunoassay. The Cylindrospermopsin sample extract and calibrators are pipetted into the test wells followed by the Cylindrospermopsin antibody into the test wells to initiate the reaction. During the 30 minutes incubation period, Cylin-drospermopsin from the sample and Cylindrospermopsin antigen compete for binding to the Cylindrosper-mopsin antibody. The Cylindrospermopsin antibody is captured on the walls of the test well. Following this 30-minute incubation, the contents of the wells are removed and the wells are washed to remove any unbound Cylindrospermopsin and free Cylindrospermopsin antibody. After wash, 1X HRP-conjugated Antibody#2 is added for 30 minutes incubation. The wells are washed afterwards, and a clear substrate is then added to the wells and any bound enzyme conjugate causes the conversion to a blue color. Following a 15-minute incubation, the reaction is stopped and the amount of color in each well is read. The color of the unknown samples is compared to the color of the calibrators and the Cylindrospermopsin concentration of the samples is derived.

Format:

• • 96-well microtiter plate (12 test strips of 8 wells)
• • Standards: 0 | 0.03 | 0.10 | 0.2| 2 ppb
• • Incubation Time: 45 Minutes
• Compatible for use with US EPA Method 546

EPA 10-Day drinking water Health Advisories for Cylindrospermopsin: 

Do not Drink – 0.7 μg/L for bottle fed infants and preschool children, pregnant and nursing woman, elderly immunocompromised and liver conditions.

Do not Drink – 3.0 μg/L for school age children to adults.

Do Not Use – 20 μg/L

EPA Draft Human Health Recreational Ambient Water Quality Criteria to protect human health: 8 μg/L.

Format: 96-well microtiter plate (12 test strips of 8 wells)
Standards: 0 | 0.03 | 0.10 | 0.2 | 2 ppb
Incubation Time: 45 Minutes
Compatible for use with US EPA Method 546