K-SDAM
SKU: 700004338
200 assays per kit
Content: | 200 assays per kit |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 1 year under recommended storage conditions |
Analyte: | Starch Damage |
Assay Format: | Spectrophotometer |
Detection Method: | Absorbance |
Wavelength (nm): | 510 |
Signal Response: | Increase |
Limit of Detection: | 0.5 g/100 g |
Total Assay Time: | ~ 40 min |
Application examples: | Cereal flours and other materials. |
Method recognition: | AACC Method 76-31.01, ICC Standard No. 164 and RACI Standard Method |
The Starch Damage Test Kit is suitable for the determination of starch damage in wheat flour / cereal flours.
The milling of wheat causes physical damage to a proportion of the starch granules of the flour. The level of starch damage directly affects water absorption and dough mixing properties of the flour and is thus of technological significance.
See more of our starch assay kits.
Advantages
The Starch Damage Test Kit is suitable for the determination of starch damage in wheat flour / cereal flours.
This kit provides a fast and simple spin column procedure for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000), as well as fresh saliva samples.
Saliva DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream research applications including PCR, Southern Blot analysis, sequencing and microarray analysis.
Saliva represents an excellent non-invasive alternative to blood collection. Human genomic DNA extracted from buccal epithelial cells and white blood cells found in saliva can be used in various applications in diagnostics. Saliva DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Saliva DNA can also be used to diagnose particular types of infections. Isolation of DNA from saliva has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive, the samples can be collected by individuals with little training, and no special equipment is required. Norgen’s Saliva DNA Isolation Kit provides a fast and simple procedure for isolating genomic DNA from both preserved saliva samples and fresh saliva samples.
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Kit Specifications | |
Maximum Saliva Input | 0.5 mL preserved saliva 0.25 mL fresh saliva |
Average Yield from 0.25 mL of Saliva* | 3 – 7 μg |
Average Purity (OD260/280) | 1.7 – 2.1 |
Time to Complete 10 Purifications | 30 minutes |
* Average yield will depending on the donor
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
Component | Cat. RU45400 (50 preps) |
---|---|
Lysis Buffer F | 30 mL |
Proteinase K in Storage Buffer | 1.2 mL |
Binding Buffer B | 12 mL |
Wash Solution A | 18 mL |
Elution Buffer B | 15 mL |
Spin Columns | 50 |
Collection Tubes | 50 |
Elution Tubes (1.7 mL) | 50 |
Product Insert | 1 |
Clone | IHC583 |
Source | Mouse Monoclonal |
Positive Control | Breast Carcinoma, Urothelial Carcinoma |
Dilution Range | 1:200 |
GATA3 is a transcription factor important in cell proliferation, development, and differentiation. GATA3 is mostly observed in breast and urothelial carcinomas, and rarely present in other cancers such as endometrial endometrioid adenocarcinoma. Among the breast carcinomas, GATA3 has a lower expression in luminal B subtype breast carcinoma. Studies have found GATA3 expression to be associated with ER (estrogen receptor), PR (progesterone receptor), and Her2 in breast cancer cases. Urothelial carcinomas stain positively for GATA3 in invasive or high grade tumors, therefore Anti-GATA3 is useful for carcinoma diagnosis when breast and bladder are plausible.
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