DBCO-C5-NHS ester is an amine-reactive compound, which can be used to modify an amine-containing molecule in organic media. This reagent isn’t soluble in aqueous media. The extended 5-carbon atom spacer arm improves solubility in commonly used organic solvents including dichloromethane, chloroform, THF, and ethyl acetate, and it also improves derivatization efficiency and stability of conjugates. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research use only.
DBCO-C5-NHS ester is an amine-reactive compound, which can be used to modify an amine-containing molecule in organic media. This reagent isn’t soluble in aqueous media. The extended 5-carbon atom spacer arm improves solubility in commonly used organic solvents including dichloromethane, chloroform, THF, and ethyl acetate, and it also improves derivatization efficiency and stability of conjugates. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research use only.
For the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017.
Meat extract and casein provide a source of nitrogen and amino acids and sodium chloride maintain theosmotic balance. Ox bile and brilliant green act as selective agents against non-target microorganisms. Tetrathionate is generated from the sodium thiosulfate. Iodine and calcium carbonate buffer the sulfuric acid generated from tetrathionate reduction.
Ingredients | /liter |
Meat extract | 4.3g |
Enzymatic digest of casein | 8.6g |
Sodium chloride | 2.6g |
Calcium carbonate | 38.7g |
Sodium Thiosulfate (anhydrous) | 30.4g |
Ox bile | 4.78g |
pH8.0±0.2 at 25°C |
Suspend 89.4g in 1 L of purified water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks,and then cool to below 45°C. Add a vial of novobiocin sodium salt (SR0640), a vial of iodine solution and a vial of brilliant green (SR0040) into 100 mL of base medium. Mix thoroughly.
Cultural characteristics observed after incubation at 35-37°C for 24 hours
Quality control strains | Approx. Inoculum(CFU) | Expected Results |
Salmonella typhimurium ATCC14028 | 10 – 100 | ≥ 10 cfu on XLD |
Escherichia coli ATCC25922 | > 104 | ≤100 cfu on TSA |
Enterococcus faecalis ATCC29212 | > 104 | <10 cfu on TSA |
2-30℃,Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
On June 14, 2024
ISO 6579:2017 Microbiology of food and animal feeding stuffs – Horizontal method for the detection, enumeration and serotyping of Salmonella spp.
Intended Use For the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017. Principle and Interpretation……
Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the udder is mainly caused by infection of various bacteria. One of such mastitis bacteria, Streptococcus agalactiae, is highly infectious and causes mainly subclinical infections, which are not identified by the herdsman. As a result, S. agalactiae can spread widely within a herd, causing immediate loss due to reduced milk. S. agalactiae is a gram-positive bacteria belonging to the Group B streptococci. Traditional cultural identification of S. agalactiae is based on S. agalactiae being beta-hemolytic as well as presence of group B Lancefield antigen and by its ability to hydrolyze sodium hippurate.
Streptococcus agalactiae TaqMan PCR Kit, 100 reactions
Streptococcus agalactiae TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
Figure 1 / 3
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component | Cat. TM30650 (100 preps) | Cat. TM30610 (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
S. agalactiae Primer & Probe Mix | 280 μL | 280 μL |
S. agalactiae Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |
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