Introduction

A highly efficient, easily automated agarose gel or PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. The Kit can be easily used in manual and automated 96 or 384-well formats.

Details

Specifications

Features	Specifications
Main Functions	Recover DNA fragments >100bp from agarose gel(<0.3g), purification of DNA from PCR, enzymatic reaction solution or crude gDNA
Applications	PCR, NGS, chip analysis, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Agarose Gel, PCR products, enzymatic reaction solution
Sample amount	Agarose Gel: <0.3g,  PCR products: ≤50µl
Recovery	80%
Elution volume	≥30μl
Time per run	≤30 minutes

The Kit method contains magnetic particles in an optimized binding buffer to selectively bind DNA fragments (>100bp) and larger to paramagnetic beads. Excess primers, nucleotides, salts, and enzymes can be removed using a simple washing procedure.

Advantages

• High recovery efficiency – up to 80% DNA recovery
• General – recover DNA from gel or enzyme-driven reaction solutions such as PCR

Kit Contents

Contents	D500101	D500102	D500103
Purification Times	50 Preps	500 Preps	5000 Preps
Buffer GDP	30 ml	250 ml	3 x 900 ml
MagPure Particles	1.6 ml	15 ml	3 x 50 ml
Buffer DW1	20 ml	180 ml	2 x 800 ml
Elution Buffer(10mmTris, pH8.5)	10 ml	60 ml	500 ml

Storage and Stability

MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data

A highly efficient, easily automated agarose gel or PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. The Kit can be easily used in manual and automated 96 or 384-well formats.

K-CellG3

180 / 360 assays per kit

Content:	180 / 360 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	endo-Cellulase
Assay Format:	Spectrophotometer, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	400
Signal Response:	Increase
Limit of Detection:	0.05 U/mL
Reproducibility (%):	~ 3%
Total Assay Time:	~ 20 min
Application examples:	Fermentation broths, industrial enzyme preparations and biofuels research.
Method recognition:	Novel method

This product has been discontinued (read more).

The CellG3 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components; 
1) 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-D-cellotrioside (BCNPG3) and 2) thermostable β-glucosidase. The benzylidene blocking group prevents any hydrolytic action by the β-glucosidase on BCNPG3.  Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase.  The rate of formation of 2-chloro-4-nitrophenol is therefore directly related to the hydrolysis of BCNPG3 by the endo-cellulase.  The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).

Please note that a new assay kit (K-CellG5) is now available for the measurement of endo-cellulase.  The CellG5 reagent contains a cellopentaose core and exhibits vastly improved sensitivity for some cellulases.  In addition, the exchange of the benzylidene blocking group in CellG3 for 3-keto-butylidene in CellG5 improves the substrate’s water solubility significantly, allowing for a reduction in the concentration of DMSO required in the assay.  As DMSO is known to inhibit certain cellulases, this is another benefit in using CellG5.  Megazyme now recommends the use of K-CellG5 for all assays for the measurement of endo-cellulase.

Browse more cellulase and other enzyme activity assay kits.

The CellG3 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components;
1) 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-D-cellotrioside (BCNPG3) and 2) thermostable β-glucosidase. The benzylidene blocking group prevents any hydrolytic action by the β-glucosidase on BCNPG3. Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 2-chloro-4-nitrophenol is therefore directly related to the hydrolysis of BCNPG3 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).