6x10ml PRP Centrifuge Rotor

DBCO-PEG24-Maleimide is a PEG linker containing a DBCO moiety and a terminal primary maleimide group. The maleimide group will react with a thiol group to form a covalent bond, enabling the connection of biomolecule with a thiol. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG24-Maleimide is a PEG linker containing a DBCO moiety and a terminal primary maleimide group. The maleimide group will react with a thiol group to form a covalent bond, enabling the connection of biomolecule with a thiol. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Overview

• Rapid and efficient removal of abundant proteins from serum and plasma samples
• Process 10 samples in 30 minutes
• Allows for visualization of low abundance proteins
• Convenient and affordable spin column protocol
• Generic protocol allows for depletion from human and other animal samples
• Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

This kit provides a fast and simple procedure for the effective depletion of major serum proteins including albumin, α-antitrypsin, transferrin and haptoglobin from serum and plasma samples.  Such abundant proteins usually obscure less abundant proteins in gel electrophresis making them difficult to visualize and recover.  The kit reduces sample complexity to allow for convenient investigation of less abundant proteins.  In other applications such as mass spectroscopy, fractionating abundant proteins improves the resolution of less abundant proteins.  The kit is ‘generic’ in that it is not antibody-based and can thus be used to deplete abundant proteins from any starting material – human or various other animals – making it more versatile, flexible and affordable than other kits.   Resulting proteins are ready for a range of applications including 2D gel electrophoresis, SDS-PAGE, Mass Spectrometry, Protein microarrays, DIGE (Difference Gel Electrophoresis) and more.

Details

Supporting Data

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Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 2 years after the date of shipment.

endo-BCN-PEG3-amine is a click chemistry linker containing a BCN and an amine group. The BCN group can react with azide-tagged biomolecules under mile condition. The amino group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

endo-BCN-PEG3-amine is a click chemistry linker containing a BCN and an amine group. The BCN group can react with azide-tagged biomolecules under mile condition. The amino group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.