6x10ml Swing Rotor 

Overview

• Purification and enrichment of intact exosomes from plasma, serum, urine, cell culture media and saliva in less than 30 minutes.
• Versatile sample input ranging from 50 µL to 10 mL
  • Plasma/Serum Exosome Purification Mini Kit (50 µL – 1 mL Plasma/Serum)
  • Plasma/Serum Exosome Purification Midi Kit (1 mL – 4 mL Plasma/Serum)
  • Plasma/Serum Exosome Purification Maxi Kit (4 mL – 10 mL Plasma/Serum)
• Exosome purification is based on Norgen’s proprietary resin separating matrix through exosomes’ surface proteins.
• No precipitation reagents, overnight incubation, protease or coagulant treatments required
• No time-consuming ultracentrifugation, filtration or special syringes required
• Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type
• Purified exosomes are compatible with functional studies.
• Purified exosomes are free from any RNA-binding proteins
• Purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration.
• Exosomal RNA can be extracted from the purified exosomes using Norgen’s Exosomal RNA Purification technology or any other RNA extraction method.

The Plasma/Serum Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for intact exosomes from different plasma/serum sample volumes ranging from 50 µL to 10 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different plasma/serum sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin.

These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal transcripts.

NanoSight® Analysis

Exosomes enriched with Norgen’s Plasma/Serum Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration

Exosomal RNA Analysis

To purify exosomes and isolate exosomal RNA, choose the Plasma/serum Exosome Purification and RNA isolation kits. The protocol is divided into 2 parts and an aliquot of purified exosomes can be taken for applications like NTA/TEM etc. before processing them for RNA isolation. Or you can use the Exosomal RNA Isolation Kit if you’ve already purified exosomes using a Norgen kit or another method. . Exosomal RNA isolation is based on Norgen’s proprietary resin without the need for phenol extractions or carrier RNA. This RNA is ideal for gene expression analysis using RT-qPCR, microarray, or NGS and for biomarker discovery.

Details

Supporting Data

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Kit Specifications
Plasma/Serum Input (Cat. 57400)	50 μL – 1 mL
Plasma/Serum Input (Cat. 57500)	1 mL – 4 mL
Plasma/Serum Input (Cat. 57600)	4 mL – 10 mL
Size of Exosomes Purified	40 nm – 150 nm
Elution Volume	Variable depending on the plasma/serum input volume
Time to Complete 10 Purifications	15 – 30 minutes

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

Component	Cat. 57400 (50 preps)	Cat. 57500 (25 preps)	Cat. 57600 (15 preps)
Slurry E	12.5 mL	12.5 mL	12.5 mL
ExoC Buffer	8 mL	8 mL	2 x 8 mL
ExoR Buffer	12 mL	12 mL	12 mL
Mini Filter Spin Columns inserted into 2 mL tubes	50	25	15
Product Insert	1	1	1

K-FRUC

SKU: 700004285

100 assays per kit

Content:	100 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Fructan
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	410
Signal Response:	Increase
Linear Range:	2.3 to 55 µg of D-fructose or D-glucose per assay
Limit of Detection:	0.16 g/100 g
Total Assay Time:	~ 90 min
Application examples:	Flours, infant formula, animal feed, pet foods, plant materials (e.g. onion), food products and other materials
Method recognition:	AACC Method 32-32.01, AOAC Method 999.03, AOAC Method 2016.14, AOAC Method 2018.07 and CODEX Method Type III

The Fructan Assay Kit is suitable for the specific measurement of fructan in plant extracts, animal feed and food products containing starch, sucrose and other sugars. It is used in three validated methods for the determination of fructan: AOAC method 999.03 (foods), AOAC method 2018.07 (Animal Feed) and AOAC method 2016.14 (infant formula and adult nutritionals).

New, improved procedure.

In the most recent development, a recombinant endo-levanase has been incorporated into the fructanase mixture, extending the use of the method to the measurement of levan-type fructans as are present in grasses such as timothy, cocksfoot, ryegrass and red fescue.

The method described in this booklet employs ultra-pure, recombinant enzymes and specifically measures fructans including inulin-type fructans from chicory, dahlia, jerusalem artichoke; highly branched fructans from onion and wheat stems and leaves; and levan-type fructans from pasture grasses such as timothy grass. The enzymes employed are completely devoid of contaminating enzymes active on β-glucan or gluco-oligosaccharides.

Browse our full range of polysaccharide assay kits.

Validation of Methods

Advantages

• Very cost effective 
• All kit reagents stable for > 2 years after preparation 
• Unaffected by high sucrose / reducing sugar concentrations  
• Fructan kits are only available from Megazyme 
• Simple format 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included

The Fructan Assay Kit is suitable for the specific measurement of fructan in plant extracts, animal feed and food products containing starch, sucrose and other sugars. It is used in three validated methods for the determination of fructan: AOAC method 999.03 (foods), AOAC method 2018.07 (Animal Feed) and AOAC method 2016.14 (infant formula and adult nutritionals).

• Taq 2x PCR Master Mix – ready to use mix simplifies your PCR setup. Only primers and template need to be added as the mix contains all copmponents for a successful and reliable PCR. This ensures reproducible results, significantly reduces set-up times and the risk of pipetting errors. The robustness of PCR performance allows the application of this mix in a wide range of PCR amplifications. For research use and further manufacturing.In case you are aiming to use our RUO products as components or for your development of e.g. an IVD medical device, please contact us.

Broad Amplification Range

Different-sized amplicons from 3 ng of a DNA plasmid were amplified. The use of Taq DNA polymerase resulted in clean and high yield of products, as analysed after PCR on a 0.8% agarose gel.

Faster Detection and Higher Sensitivity

A fragment (64 bp) of the human blood-coagulation factor IIa (F2) was amplified from 20 ng, 2 ng, 200 pg and 20 pg of a human genomic DNA extract. The same experiment was performed in parallel using the Taq DNA polymerase mix from another well-established and known supplier. PCR products were subsequently analysed on a 2.5% agarose gel.

Taq 2x PCR Master Mix – ready to use mix simplifies your PCR setup. Only primers and template need to be added as the mix contains all copmponents for a successful and reliable PCR. This ensures reproducible results, significantly reduces set-up times and the risk of pipetting errors. The robustness of PCR performance allows the application of this mix in a wide range of PCR amplifications.