The RNA Clean Beads use paramagnetic bead technology for high-throughput purification of RNA or cDNA from in vitro applications such as transcription, antisense RNA (aRNA) amplification and RNA and cDNA probe synthesis. The resulting purified product can be used in the following applications: PCR and RT-PCR, probes for microarray or macroarray, RNase protection assays, transfection for RNAi experiments and cDNA synthesis and labeling.
Specifications
| Features | Specifications |
| Main Functions | Recovery of RNA from RNA reaction solution by magnetic bead method (Replace Beckmen RNAClean XP) |
| Applications | Advanced applications, such as sequencing, genechip, fluorescence quantification, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | RNA products, restriction endonuclease systems, or other enzymatic reaction solutions |
| Sample amount | 20-100µl |
| Recovery | 90% |
| Elution volume | ≥20μl |
| Operation time | ≤50 minutes |
RNA Clean utilizes an optimized buffer to selectively bind RNA or cDNA to paramagnetic beads. Excess oligonucleotides, nucleotides, salts, and enzymes can be removed using a simple washing procedure.
Advantages
Kit Contents
| Contents | BRP-5 | BRP-50 | BRP-500 |
| RNA Clean Beads | 5 ml | 50 ml | 500 ml |
Storage and Stability
Store at 4℃ upon arrival, for up to 12 months. For best results shake the reagent well until all of the beads are completely in suspension and aliquot RNAClean Beads into RNase free containers. Do not pour remaining reagent back into the storage container. Mix RNAClean Beads well before use. The reagent should appear homogenous and consistent in color.
DO NOT FREEZE.
The RNA Clean Beads use paramagnetic bead technology for high-throughput purification of RNA or cDNA from in vitro applications such as transcription, antisense RNA (aRNA) amplification and RNA and cDNA probe synthesis. The resulting purified product can be used in the following applications: PCR and RT-PCR, probes for microarray or macroarray, RNase protection assays, transfection for RNAi experiments and cDNA synthesis and labeling.
The Norgen PCR Ranger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our PCR Ranger DNA Ladder contains eleven discrete fragments ranging from 50 bp to 1000 bp with a higher intensity reference band at 500 bp. This Ladder is ideal for assessment of a range of PCR product sizes.
Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Figure 1 / 1
Click for expanded view
PCR Ranger 100 bp DNA Ladder (Cat# 11300) – 100 loads
Ladder Properties:
• Eleven discrete bands, ranging from 50 bp to 1,000 bp
• Higher intensity band at 500 bp for easy reference
| Fragment | Size (bp) | Mass (ng) |
| 1 | 1000 | 91 |
| 2 | 900 | 66 |
| 3 | 800 | 61 |
| 4 | 700 | 51 |
| 5 | 600 | 43 |
| 6 | 500 | 70 |
| 7 | 400 | 28 |
| 8 | 300 | 23 |
| 9 | 200 | 30 |
| 10 | 100 | 22 |
| 11 | 50 | 15 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Description
AccuBand™ 50 bp DNA Ladder II is composed of 13 individual DNA fragments, presenting 1k, 900, 800, 700, 600, 500, 400, 300, 250, 200, 150, 100 and 50 bp sharp bands respectively. This product contains 2 enhanced bands (500 bp and 250 bp) for easy band identification. AccuBand™ 50 bp DNA Ladder II is ready-to-use, containing loading buffer with a tracking dye (orange G). AccuBand™ 50 bp DNA Ladder II provides a sufficient amount of DNA for clear observation of all DNA bands ranging from 50 bp to 1 kb, either in agarose gel or in polyacrylamide gel electrophoresis.
Features
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
50 ~ 1,000 bp
Concentration
55.5 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Room temperature for 6 months
4°C for 12 months
-20°C for 36 months
AccuBand™ 50 bp DNA Ladder II is composed of 13 individual DNA fragments, presenting 1k, 900, 800, 700, 600, 500, 400, 300, 250, 200, 150, 100 and 50 bp sharp bands respectively. This product contains 2 enhanced bands (500 bp and 250 bp) for easy band identification. AccuBand™ 50 bp DNA Ladder II is ready-to-use, containing loading buffer with a tracking dye (orange G). AccuBand™ 50 bp DNA Ladder II provides a sufficient amount of DNA for clear observation of all DNA bands ranging from 50 bp to 1 kb, either in agarose gel or in polyacrylamide gel electrophoresis.