6x2400ml Swing Rotor 

Overview

• Quantified standard to be used as a positive control or PCR quantification standard
• Vigorously quantified using multiple methods
• Includes partial sequence of STX-1 subunit A, and STX-2 subunit A genes (please refer to product insert for more details)
• Compatible with E. coli O157:H7 TaqMan PCR Detection Kit and E. coli O157:H7 TaqMan PCR Detection Probe/Primer and Control Set

E. coli O157:H7 is a rod-shaped, gram negative bacterium. It is an enterohemorrhagic strain of the common E. coli bacterium and infection by the O157:H7 strain is commonly associated with hemorrhagic colitis. E. coli O157:H7 is recognized by its somatic (cell wall) antigen (O157) and its flagella antigen (H7). In addition, E. coli O157:H7 is known to produce Shiga-like toxins, which cause severe symptoms. While most patients can recover from the infection, up to 15% of the patients may develop hemolytic uremic syndrome, a type of kidney failure that could be fatal. Infection of E. coli O157:H7 usually results from consumption of poorly prepared food including undercooked meat (particularly ground beef), untreated water or raw unpasteurized milk.

Norgen’s E. coli O157:H7 Quantified Bacterial DNA Standards are cloned fragments of the two Shiga-like toxin regions purified using Norgen’s sample preparation technology. They include partial sequences of STX-1 subunit A gene and STX-2 subunit A gene. Please refer to Appendix A for sequence information. The purified DNA is quantified vigorously using multiple methods including spectrophotometry, gel densitometry and real-time PCR. It is intended to be used as positive controls or PCR quantification standards for E. coli O157:H7. This product is compatible with Norgen Biotek’s E. coli O157:H7 Taqman PCR Detection Kit (Cat. TM41350) and E. coli O157:H7 Taqman PCR Detection Probe/Primer and Control Set (Cat. TM41310).

Details

Supporting Data

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Volume Provided	250 μL (for STX1)250 μL (for STX2)
DNA Quantity	2 x 104 copies per μL

Storage Conditions
Upon receipt, store Norgen’s E. coli O157:H7 Quantified Bacterial DNA Standards at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.

For scaling up and fully automating end-to-end NGS library prep

With the Flex NGS Workstation, you can automate your NGS workflow at the scale you need to increase efficiency, reduce errors, and save hands-on time. The Flex enables you to automate your library prep and pre-sequencing workflows using any leading reagent system on the market, including fragmentation and tagmentation protocols.

Optional add-ons can be purchased at a 10% discount when ordered with the Flex NGS Workstation*

For scaling up and fully automating end-to-end NGS library prep

With the Flex NGS Workstation, you can automate your NGS workflow at the scale you need to increase efficiency, reduce errors, and save hands-on time. The Flex enables you to automate your library prep and pre-sequencing workflows using any leading reagent system on the market, including fragmentation and tagmentation protocols.

Optional add-ons can be purchased at a 10% discount when ordered with the Flex NGS Workstation*

ssDNA Quantification Kit

The ssDNA Quantification Kit is developed for single stranded DNA quantification. The kit includes ssDNA Dye, ssDNA Dilution Buffer, and two ssDNA Standards. Simply dilute the ssDNA Dye with the ssDNA Dilution Buffer, add DNA sample (volume from 1-20 μL), then read the concentration using the Qubit® Fluorometer. The assay is accurate for DNA concentrations from 50 pg/µL to 200 ng/µL based on the line corresponding of the data to standards.

Our kit detects ssDNA by using fluorescent dye that enables sensitive single stranded DNA quantification , including ssDNA viruses, synthetic ssDNA, first-strand cDNA synthesis, denatured DNA, and bisulfate-converted DNA etc. ssDNA quantification is essential for the study of the biological process involves ssDNA.

Features

• • • Optimized for use with the Qubit® Fluorometer
    • Uses the Qubit® ssDNA assay setting
    • Linear range: 1-200 ng ssDNA
    • Cost saving by more than 50%

A series of input ssDNA (200, 400, 600, 800, 1000, and 1200 ng) was used.

The performance of the BioDynami ssDNA Quantification Kit is nearly identical to that of Thermo Fisher’s Qubit ssDNA kit (figure below).

Comparison of BioDynami ssDNA Quantification Kit with Thermo Fisher kit.

Common contaminants such as salts, solvents, or detergents are well tolerated in the assay (Table 1).

Contaminants has been tested in BioDynami ssDNA Kit.

The ssDNA Quantification Kit is developed for single stranded DNA quantification. The kit includes ssDNA Dye, ssDNA Dilution Buffer, and two ssDNA Standards. Simply dilute the ssDNA Dye with the ssDNA Dilution Buffer, add DNA sample (volume from 1-20 μL), then read the concentration using the Qubit® Fluorometer. The assay is accurate for DNA concentrations from 50 pg/µL to 200 ng/µL based on the line corresponding of the data to standards.