Opentrons OT-2 Tips, 300µL. Optimized for volumes 5µL to 300µL Clear Tips, Polypropylene. These tips are DNAse/RNAse, pyrogen and protease free – they are sterilized from ebeam irradiation. Not autoclavable.
Compatible with: Opentrons 8-Channel GEN2 P300 Electronic Pipettes, Opentrons Single-Channel GEN2 P300 Electronic Pipettes, Opentrons Single-Channel GEN1 P50 Electronic Pipettes, Opentrons Single-Channel GEN1 P300 Electronic Pipettes, Opentrons 8-Channel GEN1 P50 Electronic Pipettes, Opentrons 8-Channel GEN1 P300 Electronic Pipettes.
Opentrons OT-2 Tips, 300µL. Optimized for volumes 5µL to 300µL Clear Tips, Polypropylene. These tips are DNAse/RNAse, pyrogen and protease free – they are sterilized from ebeam irradiation. Not autoclavable.
Compatible with: Opentrons 8-Channel GEN2 P300 Electronic Pipettes, Opentrons Single-Channel GEN2 P300 Electronic Pipettes, Opentrons Single-Channel GEN1 P50 Electronic Pipettes, Opentrons Single-Channel GEN1 P300 Electronic Pipettes, Opentrons 8-Channel GEN1 P50 Electronic Pipettes, Opentrons 8-Channel GEN1 P300 Electronic Pipettes.
This product provides a reliable solution for DNA isolation from yeast samples. Total DNA can be purified from yeast (<5x 107) without phenol or chloroform. The whole extraction can be finished within 60 minutes. Purified DNA can be directly used for PCR, Southern blot, ect.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from yeast cultures |
Applications | PCR, southern blot and enzyme digestion, etc. |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Yeast culture |
Sample amount | Bacterial culture: 1-1.5 ml |
Elution volume | ≥30μl |
Time per run | ≤60 minutes |
Liquid carrying volume per column | 800μl |
Binding yield of column | 100μg |
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris,pH9.0, 0.5mm EDTA).
Kit Contents
Contents | D314702 | D314703 |
Purification Times | 50 Preps | 250 Preps |
Hipure DNA Mini Columns I | 50 | 250 |
2ml Collection Tubes | 50 | 250 |
Glass Beads (0.4~0.6mm) | 20 g | 90 g |
Buffer SE | 30 ml | 150 ml |
Lyticase | 1.8 ml | 5 x 1.8 ml |
Buffer ATL | 30 ml | 150 ml |
ReagentDX | 500 μl | 1500 μl |
Buffer DL | 30 ml | 150 ml |
Buffer GW1* | 13 ml | 66 ml |
Buffer GW2* | 20 ml | 2 x 50 ml |
Proteinase K | 12 mg | 60 mg |
Protease Dissolve Buffer | 1.8 ml | 5 ml |
Buffer AE | 15 ml | 30 ml |
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. Buffer ATL may precipitate at low temperature. Dissolve it by 37℃ water bath.
This product provides a reliable solution for DNA isolation from yeast samples. Total DNA can be purified from yeast (
Clone | IHC125 |
Source | Mouse Monoclonal |
Positive Control | Ovarian Serous Carcinoma |
Dilution Range | 1:200 |
Annexin A1 (ANXA1) is a membrane protein that plays a role in innate and adaptive immunity by controlling the biosynthesis of inflammation, prostaglandins, and leukotriene mediators. This target is overexpressed in 97% of all samples from patients with with hairy cell leukemia, and is absent in other B-cell lymphomas. High ANXA1 expression is frequently associated with advanced stage esophageal and esophagogastric junction adenocarcinoma, and is also linked to advanced and metastatic disease states.
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