6x9ml Cyto Swing Rotor

Overview

• Detection kits for Listeria monocytogenes
• CE-IVD marked version available for in vitro diagnostic use
• Available in TaqMan format for analysis.

Listeria monocytogenes have emerged as significant foodborne pathogens that pose a serious public health problem. As the causative agent of Listeriosis, L. monocytogenes has the highest rate of fatality rate among all foodborne pathogens. L. monocytogenes is a facultatively intracellular, Gram-positive bacterium that could survive high and low temperatures, low pH. It is a rare causative agent of mastitis in cow. However, due to its ability to resist various food processing technologies as well as to grow at low temperature, L. monocytogenes is know to be associated with both raw and pasteurized milk, as well as dairy products such as cheese. As little as 1000 organisms may cause the disease with pregnant, new-born, and immunocompromised individuals the most susceptible.

Listeria monocytogenes TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Listeria monocytogenes TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Component	Cat. TM30450 (100 preps)	Cat. TM30410 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
Listeria monocytogenes Primer & Probe Mix	280 μL	280 μL
Listeria monocytogenes Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

96-Well 0.2 mL PCR Strip Rack SBS/ SLAS Footprint

SKU: MTP96

The Permagen 96-well 0.2 mL PCR Strip Rack was designed for use in manual or automation applications where strip tubes are required. It can also be used with our MSP750 MSP750R MSPU650R or MSPU650 magnet plates and is available as a discounted kit

The Permagen 96-well 0.2 mL PCR Strip Rack was designed for use in manual or automation applications where strip tubes are required. It can also be used with our MSP750 MSP750R MSPU650R or MSPU650 magnet plates and is available as a discounted kit

Introduction

Usages:

 For selective isolation and culture of Pseudomonas aeruginosa.

Principle:

Acetamidase bacteria can use acetamide as a carbon source and acetamide decomposition Alcaligenes; NaCl maintain osmotic balance; dipotassium phosphate and potassium dihydrogenphosphate as buffer; magnesium ions provide necessary growth ; agar medium as coagulant; phenol red as a pH indicator.

Formulation:

Acetamide 10g

NaCl 5g

Dipotassium hydrogen phosphate 1.39g

Potassium dihydrogen phosphate 0.73g

Magnesium sulfate 0.5g

Phenol red 0.012g

Agar 15g

Final pH 7.2 ± 0.2

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

100g