The Norgen MiniSizer 50 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains fourteen discrete fragments ranging from 25 bp to 650 bp in 50 bp increments with double intensity reference bands at 200 bp and 400 bp. The MiniSizer is ideal for PCR product size confirmation.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Figure 1 / 1
Click for expanded view
MiniSizer 50 bp DNA Ladder (Cat# 11200) – 100 loads
Ladder Properties:
• Fourteen discrete bands, ranging from 50 bp to 650 bp
• Higher intensity bands at 200 bp and 400 bp for easy reference
Fragment | Size (bp) | Mass (ng) |
1 | 650 | 61 |
2 | 600 | 46 |
3 | 550 | 36 |
4 | 500 | 30 |
5 | 450 | 35 |
6 | 400 | 85 |
7 | 350 | 27 |
8 | 300 | 25 |
9 | 250 | 16 |
10 | 200 | 54 |
11 | 150 | 33 |
12 | 100 | 21 |
13 | 50 | 17 |
14 | 25 | 14 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
K-GLUT
SKU: 700004301
60 assays (manual) / 600 assays (microplate) / 700 assays (auto-analyser)
Content: | 60 assays (manual) / 600 assays (microplate) / 700 assays (auto-analyser) |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 1 year under recommended storage conditions |
Analyte: | L-Glutamic Acid, MSG |
Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
Detection Method: | Absorbance |
Wavelength (nm): | 492 |
Signal Response: | Increase |
Linear Range: | 0.4 to 20 µg of L-glutamic acid per assay |
Limit of Detection: | 0.21 mg/L |
Reaction Time (min): | ~ 8 min |
Application examples: | Fruit and vegetables (e.g. tomato), processed fruit and vegetables (e.g. tomato puree / juice, ketchup, soy sauce), condiments, processed meat products (e.g. extracts, bouillon and sausages), soup, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.). |
Method recognition: | Methods based on this principle have been accepted by ISO, GOST and NMKL |
The L-Glutamic Acid test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of L-glutamate (MSG) in foodstuffs.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Explore more organic acid test kits.
Advantages
The L-Glutamic Acid test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of L-glutamate (MSG) in foodstuffs.
Propargyl-PEG3-amine is a PEG linker that is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl group reacts with azides in copper catalyzed azide-alkyne Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG3-amine is a PEG linker that is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl group reacts with azides in copper catalyzed azide-alkyne Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
Copyright © 2024 A3P Scientific Co., Ltd. All rights reserved. Web by Mountain Studio
Privacy Policy | Terms of Use | Site Map