The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Kits are designed for the rapid removal of endotoxins from previously purified proteins or peptides, with protein recoveries of > 95% being achieved. Endotoxins, also known as lipopolysaccharides, are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins liberated by Gram-negative bacteria are frequent contaminations of protein solutions derived from bioprocesses. Due to the high toxicity of endotoxins in vivo and in vitro, their removal from protein preparations is often necessary prior to the use of the protein in downstream applications. These kits efficiently reduce endotoxin levels to ≤ 0.01 EU/mg of protein, using spin column chromatography based on Norgen’s proprietary resin as the separation matrix. These kits are highly efficient in removing many different salts commonly used in the laboratory including, but not limited to, MgCl2, NaCl, KCl, CaCl2, LiCl and CsCl. The purified protein samples can be used in a number of downstream applications including sequencing, cloning, and in vitro and in vivo introduction into cells and organisms for various purposes. The simultaneous removal of salts while concentrating a dilute protein solution makes the kit a convenient method for preparing proteins before running many downstream applications such as SDS-PAGE, isoelectric focusing, X-ray crystallography, NMR spectroscopy, mass spectroscopy and other applications.
Mini Kit
The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit is designed for the rapid removal of endotoxins from up to 200 μg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.
Maxi Kit
The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit is designed for the rapid removal of endotoxins from up to 4 mg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.
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| Kit Specifications | |
| Maximum Protein Input | 4 mg |
| Minimum Protein Input | 0.25 mg |
| Maximum Column Volume Input | 18 mL |
| Molecular Weight of Recovered Proteins | No Molecular Weight cut-off |
| Final Endotoxin Levels | ≤ 0.01 EU/μg protein |
| Protein Recovery | 90-95% |
| % Detergent Removal | 90-95% |
| Detergents that can be Removed | Including Triton® X-100, CHAPS, NP-40 and Tween 20 |
| Elution Volume | 2-4 mL |
| Time to Complete 10 Purifications | 30-40 minutes |
Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 22800 (25 preps) | Cat. 22200 (4 preps) |
|---|---|---|
| Binding Buffer J | 8 mL | 8 mL |
| Binding Buffer N | 4 mL | 20 mL |
| Wash Solution M | 50 mL | 130 mL |
| Wash Solution CIP | 20 mL | 60 mL |
| Wash Solution N | 30 mL | 130 mL |
| Wash Solution NIP | 20 mL | 60 mL |
| Elution Buffer G | 6 mL | 20 mL |
| Endotoxin Removal Solution | 1.5 mL | 1.5 mL |
| Protein Neutralizer EF | 2 mL | 2 mL |
| Maxi Spin Columns (assembled with Collection Tubes) | – | 4 |
| Mini Spin Columns | 25 | – |
| Collection Tubes | 25 | – |
| Maxi Spin Columns (assembled with Collection Tubes) | 4 | |
| Elution Tubes (1.7 mL) | 25 | – |
| Elution Tubes (50 mL) | 4 | – |
| Product Insert | 1 | 1 |
The BK virus is a member of the polyomavirus family. It has been suggested that this virus may be transmitted through respiratory fluids or urine, since infected individuals periodically excrete virus in the urine. BK viral infections are typically asymptomatic in healthy individuals, however very mild symptoms may appear including mild respiratory infections and fever. Infections with BK virus in immunocompromised or immunosupressed patients are much more severe and may involve renal dysfunction. In fact, in kidney transplant patients the immunosupressive drugs required for the transplant may allow the virus to replicate within the graft, resulting in a disease called BK virus nephropathy (BKVN). The JC virus is a type of human polyomavirus and is very common in the general population, infecting 70 to 90% of humans. Most people acquire JCV in childhood or adolescence. Typically the infection is subclinical and no of consequence in individuals with healthy immune systems. The initial site of infection may be the tonsils or the gastrointestinal tract, and the virus then remains latent in the gastrointestinal tract. JCV can also infect the tubular epithelial cells in the kidneys, where it continues to reproduce, shedding virus particles in the urine. Also, JCV can cross the blood-brain barrier into the central nervous system. JCV is known to cause the usually fatal progressive multifocal leukoencephalopathy (PML) by destroying oligodendrocytes in the brain in immunodeficient or immunosuppressed individuals. The JC and BK viruses are very similar, with their genomes sharing 75% homology. It is however important to differentiate between the viruses due to the differences in pathology and especially the invariably fatal outcome of PML which is only caused by the JC virus.
BKV/JCV TaqMan PCR Kit, 100 reactions
BKV/JCV TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM39350 (100 preps) | Cat. TM39310 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| BKV/JCV Primer & Probe Mix | 280 μL | 280 μL |
| BKV/JCV Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
