This kit provides a rapid method for the isolation and purification of DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE DNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The purified DNA is of high yield and integrity and is free of inhibitors, ready for use in a number of downstream applications including qPCR, mutation screening, microarray analysis, sequencing, single nucleotide polymorphism (SNP) and short-tandem repeat (STR) genotyping. The protocol can be completed in as little as 1 hour.
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| Kit Specifications | |
| Maximum Column Binding Capacity (gDNA) | 10 μg |
| Maximum Loading Volume Per Spin Column | 650 μL |
| Size of DNA Purified | All sizes > 80 bp |
| Maximum Amount of Starting Material | 5 sections < 20 µm thick paraffin slices 25 mg of unsectioned block |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 47400 (50 preps) |
|---|---|
| Digestion Buffer A | 25 mL |
| Buffer RL | 30 mL |
| Wash Solution A | 38 mL |
| Elution Buffer B | 15 mL |
| Proteinase K | 12 mg |
| RNase A | 1 tube |
| gDNA Purification Micro Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
DBCO-amine TFA salt is a simple building block containing a DBCO moiety and an amine group. Amine is very reactive with NHS ester. DBCO is commonly used for copper-free Click Chemistry reactions.
DBCO-amine TFA salt is a simple building block containing a DBCO moiety and an amine group. Amine is very reactive with NHS ester. DBCO is commonly used for copper-free Click Chemistry reactions.
