For the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017.
Meat extract and casein provide a source of nitrogen and amino acids and sodium chloride maintain theosmotic balance. Ox bile and brilliant green act as selective agents against non-target microorganisms. Tetrathionate is generated from the sodium thiosulfate. Iodine and calcium carbonate buffer the sulfuric acid generated from tetrathionate reduction.
| Ingredients | /liter |
| Meat extract | 4.3g |
| Enzymatic digest of casein | 8.6g |
| Sodium chloride | 2.6g |
| Calcium carbonate | 38.7g |
| Sodium Thiosulfate (anhydrous) | 30.4g |
| Ox bile | 4.78g |
| pH8.0±0.2 at 25°C | |
Suspend 89.4g in 1 L of purified water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks，and then cool to below 45°C. Add a vial of novobiocin sodium salt (SR0640), a vial of iodine solution and a vial of brilliant green (SR0040) into 100 mL of base medium. Mix thoroughly.
Cultural characteristics observed after incubation at 35-37°C for 24 hours
| Quality control strains | Approx. Inoculum(CFU) | Expected Results |
| Salmonella typhimurium ATCC14028 | 10 – 100 | ≥ 10 cfu on XLD |
| Escherichia coli ATCC25922 | > 104 | ≤100 cfu on TSA |
| Enterococcus faecalis ATCC29212 | > 104 | <10 cfu on TSA |
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
On June 14, 2024
ISO 6579:2017 Microbiology of food and animal feeding stuffs – Horizontal method for the detection, enumeration and serotyping of Salmonella spp.
Intended Use For the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017. Principle and Interpretation……
029010 Gram Stain
Usage:For Gram Stain Test.
Specification: 10ml*4 vials
10ml*4vials