K-EBHLG
SKU: 700004278
50 assays per kit
| Content: | 50 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | β-Glucan |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 510 |
| Signal Response: | Increase |
| Limit of Detection: | 1 g/100 g |
| Reaction Time (min): | ~ 100 min |
| Application examples: | Yeast preparations and other materials |
| Method recognition: | Novel method |
This product has been discontinued (see here), please use the recommended replacement product β-Glucan Assay Kit (Yeast and Mushroom) for all your yeast and mushroom β-Glucan testing needs.
Enzymatic Yeast Beta-Glucan test kit, an enzymatic procedure for the measurement and analysis of 1,3:1,6-β-glucan in yeast. Also measures 1,3-β-glucan.
See more of our polysaccharide test kit products.
Advantages
Standard included
Very competitive price (cost per test)
All reagents stable for > 12 months after preparation
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
This product has been discontinued (see here), please use the recommended replacement product β-Glucan Assay Kit (Yeast and Mushroom) for all your yeast and mushroom β-Glucan testing needs.
This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from tissue / blood / body fluid / swab /dry blood spots |
| Applications | PCR, qPCR, southern bolt and virus detection, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Tissue, cell, blood, saliva, swab, blood spot, semen and other clinical samples |
| Sample amount | Solid tissue : 1-10mg, Anticoagulant blood : 200μl |
| Yield | 1 – 15µg |
| Elution volume | ≥20μl |
| Time per run | 30 – 60 minutes |
| Liquid carrying volume per column | 750µl |
| Binding yield of column | 100µg |
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
| Contents | IVD3018 |
| Purification Times | 100 |
| 2ml Collection Tubes | 2 x 100 |
| Buffer ATL | 60 ml |
| Buffer AL | 60 ml |
| Buffer GW1 | 44 ml |
| Buffer GW2 | 50 ml |
| Proteinase K | 60 mg |
| Protease Dissolve Buffer | 5 ml |
| Buffer AE | 15 ml |
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Experiment Data
This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples (including serum and plasma). The kit is based on super paramagnetic particles purification technology. Purified DNA/RNA is ready for downstream applications such as Real Time PCR, biochip analysis, NGS and other related experiments.
Specifications
| Features | Specifications |
| Main Functions | Extract Pathogen RNA/DNA from 0.5ml plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution for mNGS application, remove host background nucleic acid. |
| Applications | RT-PCR，Real Time PCR, biochip analysis, NGS |
| Products | Pathogen DNA / RNA |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | low/cell-free samples such as plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution |
| Sample amount | 0.5 ml |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
Kit Contents
| Contents | R667200B | R6672-02B |
| Purification Times | 24 Preps | 96 Preps |
| 2ml Bead Tubes | 24 | 96 |
| Proteinase K | 12 mg | 50 mg |
| Protease Dissolve Buffer | 1.8 ml | 3 ml |
| Buffer SDS (20%) | 1.8 ml | 8 ml |
| MagBind Particles | 0.6 ml | 2.5 ml |
| Buffer MLB | 15 ml | 60 ml |
| Buffer MW1* | 13 ml | 44 ml |
| Buffer MW2* | 6 ml | 50 ml |
| Buffer AVE | 5 ml | 30 ml |
Storage and Stability
MagBind Particles and Proteinase K Solution should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples (including serum and plasma). The kit is based on super paramagnetic particles purification technology. Purified DNA/RNA is ready for downstream applications such as Real Time PCR, biochip analysis, NGS and other related experiments.