The Permagen Post magnet low elution plate was designed for use in manual or automation applications where volumes as low as 5 µL are required. The post magnets will create bead pellets to one quadrant of the well making aspiration easier and accidental bead disruption less likely
Detail
The Permagen Post magnet low elution plate was designed for use in manual or automation applications where volumes as low as 5 µL are required. The post magnets will create bead pellets to one quadrant of the well making aspiration easier and accidental bead disruption less likely
SBS SLAS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
Usages: For the differentiation of Gram-negative bacteria on the basis of citrate utilization.
Principle: Magnesium ions in various metabolic cofactors; ammonium dihydrogen phosphate to provide nitrogen; dipotassium hydrogen phosphate is the buffer; sodium citrate as a carbon source; agar as medium coagulant.
Formulation(per liter): Sodium chloride 5g Magnesium sulfate 0.2g Ammonium dihydrogen phosphate 0.2g Sodium ammonium phosphate 0.8g Sodium citrate 2g Agar 15g Bromothymol blue 0.08g Final pH 7.0 ± 0.2
How to use: 1.Suspend 23.3g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 autoclave for 15min. 2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Fifteen discrete fragments ranging from 300 bp to 24000 bp
Higher intensity reference bands at 5000 bp and 10000 bp
The Norgen UltraRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our UltraRanger contains fifteen discrete fragments ranging from 300 bp to 24000 bp with higher intensity reference bands at 5000 bp and 10000 bp. This ladder is ideal for sizing digested DNA of high molecular weight.
Contents 1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
UltraRanger 1kb DNA Ladder (Cat# 12100) – 100 loads
Ladder Properties: • Fifteen discrete fragments ranging from 300 bp to 24000 bp
• Higher intensity reference bands at 5000 bp and 10000 bp
Fragment
Size (bp)
Mass (ng)
1
24000
30
2
14000
27
3
10000
60
4
8000
44
5
6000
36
6
5000
55
7
4000
49
8
3000
39
9
2500
35
10
2000
30
11
1500
33
12
1000
10
13
700
21
14
500
18
15
300
11
Recommended Use: Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage: Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Fractionate leukocytes from whole blood in minutes with provided RBC Lysis Buffer
Isolate total RNA, including microRNA, without phenol
Rapid and convenient spin-column format and 96-well plate for high throughput applications
Purified RNA is ready for any downstream application including RT-PCR, qRT-PCR, NGS, arrays and more. Find out more information on Norgen’s NGS services
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
These kits provide a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from mammalian blood samples. These kits are supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation. Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. These kits are able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.
Leukocyte RNA Purification Kit (Spin Column)
This kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples in 40 minutes. Allowable blood input ranges from 10 μL to 2 mL or 3 x 106 Leukocytes
Leukocyte RNA Purification Plus Kit (Plus)
Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. Complete 10 purifications in as little as 40 minutes.
Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Norgen’s kit allows for the isolation of total leukocyte RNA, including all small RNA species. The purified RNA is of the highest quality and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension, and expression array assays. Allowable blood input ranges from 10 μL to 1 mL or 1.5 x 106 Leukocytes.
All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.
