96×5ml Swing Buckets Rotor

Product Details

Sample Type:

Serum, Plasma, Whole Blood

Package Size:

48 Tests/Kit

Test Time:

5-20 Minutes

Target Disease:

African Swine Fever Virus

Product Name:

African Swine Fever Virus Isothermal Detetction Fluorescence Kit

Test Principle:

Nucleic Acid Detection

Target Species:

Pigs

High Light:

Livestock porcine detection kit

, 

porcine detection kit 48 Tests/Kit

, 

5-20 Minutes porcine test kit

Product Description

Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection 

Product parameters:

Kit composition (WLRE8208KIT 48T/Pack)
Composition	Content
E buffer	1 mL×2 Tubes
B buffer	150 μL×1Tube
Positive control template	100 μL×1Tube
Reagents	48T

Product features and advantages:

Product features and advantages
Features	Advantages
High sensitivity	1 copies/ml
Strong specificity	Specificity is superior to PCR
Short reaction time	20min
Polymorphic reagent	Liquid, freeze-dried powder, freeze-dried ball
Easy to operate	Few steps of liquid dispensing; It is even possible to add only samples and amplify to get the resultsDesign of primers and probe is simple
Easy to store and transport	It is best preserved at -20℃, and can be stored at room temperature for up to 30 days in a cool place away from light.The freeze-dried form has a long storage time and is convenient for transportation
Low requirements on equipment	Applicable to Applied Biosystems 7500, QuantStudio 3/5, QuantStudio 6/7 Flex fluorescence quantitative PCR instrument;Applicable to our WL-16-III and other isothermal fluorescence detector

Product application:

Application
Ultra-clean laboratory	African Swine Fever Virus Isothermal Detection
Indoor

Operation procedure and process:

Operation procedure and process:

Take out the liquid components of the kit in advance, thaw at room temperature, and shake to mix.

Prepare freeze-dried reagents according to the number of samples to be tested, negative and positive controls, and add 37.5 μL E buffer to each freeze-dried reagent.

Select the appropriate nucleic acid extraction method and reagent to extract the sample nucleic acid

Add 10 μL nucleic acid template to the reaction tube (the amount of template can be adjusted to be filled with sterile water; That is, 10 μL nucleic acid template plus sterile water), 10 μL positive control template was added to positive control, and 10 μL sterile water was added to negative control

Add 2.5μL B buffer to each reaction tube and close the tube cap (for multiple reactions, it is recommended to add B buffer to the inside of the tube cap)

Thoroughly mix the reaction tube upside and down for 8-10 times. After mixing, shake (or rapidly centrifuge) the reaction liquid to the bottom of the tube and transfer it to the amplification zone.

Performence Comparison Data:

No.	TT value	Template concentration	Result determination
1	4.2	10-1	Positive
2	5.5	10-2	Positive
3	10.2	10-3	Positive
4	17.0	10-4	Positive
5	12.0	10-5	Positive
6	–	10-6	Negative
7	–	10-7	Negative
8	Negative control		Negative

Support and Services:

Livestock Disease Kit Technical Support and Services

We provide technical support and services for our Livestock Disease Kit. We are committed to helping you get the most out of your product and ensuring your satisfaction with our products and services.

• Our technical support staff is available to answer any questions you may have about the product.
• We offer online tutorials and user guides to help you understand the product better.
• We provide technical assistance to help troubleshoot any issues you may be having with the product.
• We offer a warranty on our products.

If you have any questions or need technical support, please do not hesitate to contact us. We are here to help you make the most of your Livestock Disease Kit.

FAQ:

• Q: What diseases can Livestock Disease Kit detect?
  A: Livestock Disease Kit can detect different infectious diseases, such as Foot and Mouth Disease, Bluetongue Disease, Bovine Viral Diarrhea.
• Q: How does Livestock Disease Kit work?
  A: Livestock Disease Kit uses advanced MIRA technology to amplify the target DNA/RNA targent sequences , and detect the disease by analyzing the amplified sequences.
• Q: How long does it take to get results from Livestock Disease Kit?
  A: Livestock Disease Kit can give a result within 5-20 mins.
• Q: What type of sample is suitable for Livestock Disease Kit?
  A: Livestock Disease Kit is suitable for blood, serum, and tissue samples.
• Q: Does Livestock Disease Kit require professional equipment?
  A: No, Livestock Disease Kit does not require any professional equipment,can use with our isothermal equipment or pcr machine

Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection

OverView

AZscript™ Reverse Transcriptase (RT) is an RNA-dependent DNA polymerase used to synthesize complementary DNA (cDNA) from an RNA template.

While originating from Moloney Murine Leukemia Virus (M-MLV), AZscript RT has been engineered for increased thermostability and reduced RNase H activity.

In cDNA synthesis a higher reaction temperature can reduce RNA secondary structures and improve efficiency and specificity of the reaction.

Furthermore, a reduction in RNase H activity increases performance of the cDNA synthesis, especially for longer transcripts.

The combination of these tailored features ensures that AZscript Reverse Transcriptase provides an effective and reliable solution for various cDNA synthesis applications.

Key Features 

• Increased thermostability.
• Reduced RNase H activity.

Applications

• cDNA synthesis
• Demonstrated performance in RT-qPCR
  

Figures

AZscript™ Reverse Transcriptase (RT) is an RNA-dependent DNA polymerase used to synthesize complementary DNA (cDNA) from an RNA template.

Description

This kit provides a single tube to screen for the presence of high-risk HPV types, HPV16, HPV18, HPV31, HPV33, HPV35, HPV39, HPV45, HPV51, HPV52, HPV56, HPV58, HPV59, HPV66 and HPV68. The multiplex test is detected in two fluorescent channels differentiating between HPV16 / HPV18 which both produce a VIC channel signal and all others which produce a signal in the FAM channel. HPV16 and HPV18 account for 70% of positive findings in clinical practice so it is helpful to know if either of these are present. All other high risk genotypes together make up the remaining 30% of clinical positives and are grouped together into the FAM channel. In this configuration, the kit gives a partial genotyping result and some additional information on which high risk strains are present.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings