This product allows rapid and reliable isolation of high-quality genomic DNA from various soil, stool, and other environmental samples. Up to 500mg soil, 100mg stool, or 0.5g environmental samples can be processed in 60 minute. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 250-500mg soil sample |
| Applications | PCR, southern blot and enzyme digestion, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Soil |
| Sample amount | 250-500mg |
| Elution volume | ≥50μl |
| Time per run | ≤70 minutes |
This product is based on the purification method of high binding magnetic particles. Soil sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteins, polysaccharides, and other contaminants are removed using our propietary Absorber Solution. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing buffer to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Kit Contents
| Contents | D635601 | D635602 | D635603 |
| Purification Times | 48 Preps | 96 Preps | 5 x 96 Preps |
| MagPure Particles | 2.5 ml | 5 ml | 22 ml |
| 2ml Bead Tubes | 48 | 96 | 400 |
| Buffer SOL | 60 ml | 100 ml | 500 ml |
| Buffer SDS | 6 ml | 10 ml | 50 ml |
| Reagent DX | 1 ml | 1 ml | 5 ml |
| Buffer PS | 10 ml | 20 ml | 90 ml |
| Absorber Solution | 10 ml | 20 ml | 90 ml |
| Buffer GDP | 70 ml | 150 ml | 2 x 350 ml |
| Buffer GW1* | 22 ml | 44 ml | 220 ml |
| Elution Buffer | 20 ml | 20 ml | 60 ml |
Storage and Stability
MagPure Particles and Absorber Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
This product allows rapid and reliable isolation of high-quality genomic DNA from various soil, stool, and other environmental samples. Up to 500mg soil, 100mg stool, or 0.5g environmental samples can be processed in 60 minute. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.
Description
The ExcelTaq™ 5X PCR Master Dye Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as ready-to-use master mix for virtually all PCR applications. The mixture contains all components for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Master Dye Mix is supplied as a 5X concentrated ready-to-use mix, that is a mixture of recombinant Taq DNA polymerase, reaction buffer, MgCl2, dNTP, enzyme stabilizer and PCR-friendly loading dye solution containing tracking dye (Bromophenol blue) enabling efficient amplification of template in PCR and allows the user to prepare a PCR reagent – loading dye master mix conveniently.
Features
Applications
Storage
4°C for 6 months
-20°C for 24 months
The ExcelTaq™ 5X PCR Master Dye Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as ready-to-use master mix for virtually all PCR applications. The mixture contains all components for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Master Dye Mix is supplied as a 5X concentrated ready-to-use mix, that is a mixture of recombinant Taq DNA polymerase, reaction buffer, MgCl2, dNTP, enzyme stabilizer and PCR-friendly loading dye solution containing tracking dye (Bromophenol blue) enabling efficient amplification of template in PCR and allows the user to prepare a PCR reagent – loading dye master mix conveniently.
Description
ExcelRT™ Reverse Transcription Kit is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with oligo (dT)20 and random hexamers, which are used to synthesize cDNA from poly(A) tailed mRNA and total RNA, respectively.
Features
Application
Storage
-20°C for 24 months
| ComponentVolume ExcelRT™ Reverse Transcriptase (200 U/μl) 100 μl RNase Inhibitor (20 U/μl)100 μl 5X RT Buffer (DTT)500 μl dNTPs Mix (10 mM each)200 μl Oligo (dT)20 (50 μM)100 μl Random Hexamers (100 μM)100 μl DEPC-Treated H2O 1 ml x 2 |
Storage Buffer
Reverse Transcriptase: 20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer and 50% (v/v) glycerol
RNase Inhibitor: 40 mM HEPES-KOH (pH 7.5), 100 mM KCl, 8 mM DTT, 0.1 mM EDTA, stabilizer and 50% (v/v) glycerol
5X RT buffer (DTT)
250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2 and 50 mM DTT
Storage
-20°C for 24 months
ExcelRT™ Reverse Transcription Kit is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with oligo (dT)20 and random hexamers, which are used to synthesize cDNA from poly(A) tailed mRNA and total RNA, respectively.