Alkyne-ethyl-PEG1-t-Butyl ester

Overview

• Detection kits for Giardia intestinalis
• CE-IVDD marked in accordance with the European Commission Directive 98/79/EC.
• Research version available
• Available in Taqman format for analysis

Giardiasis is a disease of the small bowel caused by the protozoan parasite Giardia intestinalis (syn.duodenalis or lamblia). Giardia is one of the most common intestinal parasites in the world, and occurs at very high prevalence rates in places with poor water sanitation. Individuals become infected through ingesting or coming into contact with contaminated water, food or soil. It can also be spread through the faecal-oral route due to poor hygiene practices, which makes it common in day-care centers. G. intestinalis lives inside the intestines of infected humans or other animals including cats, dogs, birds, cows, beaver and deer. Symptoms of infection include diarrhea, malaise, excessive gas, bloating, nausea, diminished interest in food, possible vomiting and weight loss.

Giardia intestinalis TaqMan RT-PCR Kit, 24 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Details

Supporting Data

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Storage Conditions

The Giardia intestinalis TaqMan RT-PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. DxTM43800 (24 rxns)
MDx TaqMan 2X RT-PCR Master Mix Dx	550 µL
Giardia intestinalis Primer & Probe Mix Dx	2 x 70 µL
Giardia intestinalis Positive Control Dx – 200,000 copies/μL	50 µL
Nuclease-Free Water	2 x 1.25 mL
Product Insert	1

Interested in the analysis of DNA or RNA modifications? Then this DNA polymerase could help to analyze such modifications. The m6A sensitive DNA polymerase  exhibits increased misincorporation rates opposite m6A, while unmodified adenine is not affected. This prevents the loss of methylation information during reverse transcription and thus allows direct m6A sequencing. For further information refer to the original publication.   

Available upon request and for R&D use only – Contact Us   

The m6A sensitive DNA polymerase is supplied as a 5 µM solution containing glycerol and is supplied together with 10x reaction buffer. 

The enzyme can also be used for real-time cycling, when adding a suitable dye.

Interested in the analysis of DNA or RNA modifications? Then this DNA polymerase could help to analyze such modifications. The m6A sensitive DNA polymerase exhibits increased misincorporation rates opposite m6A, while unmodified adenine is not affected. This prevents the loss of methylation information during reverse transcription and thus allows direct m6A sequencing. For further information refer to the original publication.

Cell Culture Dish 150mm

Cell Culture Dish provide Excellent surface smoothness gives clear microscopic observation

Specifications: 35mm. 60mm.100mm.150mm

PRODUCT FEATURES

• The product is made of medical grade USP CLASS VI polymer polystyrene
• The product is made under a 100,00- class dust-free manufacturing site
• Two kinds of product line up are providing.

For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.

For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.

• Gamma radiation sterilization
• Non-Pyrogenic, DNase/Rnase free.

Cell Culture Dish provide Excellent surface smoothness gives clear microscopic observation

Specifications: 35mm. 60mm.100mm.150mm