Alkyne-PEG2-iodide

Overview

• Isolate genomic DNA from all types of bacteria (both Gram-positive and Gram-negative)
• Rapid and convenient spin column protocol
• 96-well format available for high throughput
• High yield, high quality DNA for sensitive downstream applications including sequencing, PCR, qPCR and more

This kit is designed for the rapid spin column preparation of genomic DNA from 2 x 10⁹ viable bacterial cells (between 0.5 and 1.0 mL of culture). This kit can be used for both Gram-negative and Gram-positive bacteria including Escherichia coli and Bacillus cereus. Purified genomic DNA is of an excellent quality and yield, and is fully compatible with restriction enzyme digestions, sequencing, PCR, qPCR and more. Also available in 96-well format for high throughput applications.

Details

Supporting Data

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Kit Specifications (Spin Columns)
Input	2 x 109 bacterial cells
Column Binding Capacity	25 µg
Average Yield*	Up to 20 µg
Time to Complete 10 Purifications	1 hour

* Yield will vary depending on the type of sample processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

Component	Cat. 17900 (50 preps)	Cat. 17950 (192 preps)
Resuspension Solution A	20 mL	60 mL
Lysis Buffer P	18 mL	60 mL
Solution BX	28 mL	110 mL
Wash Solution A	18 mL	2 x 38 mL
Elution Buffer B	30 mL	2 x 30 mL
Proteinase K	12 mg	50 mg
Spin Columns	50	–
96-Well Plate	–	2
Adhesive Tape	–	4
Collection Plate	–	2
Collection Tubes	50	–
Elution Tubes (1.7 mL)	50	–
Elution Plate	–	2
Product Insert	1	1

Sulfo DBCO-PEG3-NHS ester is a water soluble, click chemistry reagent which can undergo copper free click chemistry Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Sulfo DBCO-PEG3-NHS ester is a water soluble, click chemistry reagent which can undergo copper free click chemistry Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.