Alkyne-PEG2-iodide is a crosslinker containing a propargyl group and an iodine group. The propargyl group can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry reactions. Iodine (I) is a very good leaving group for nucleophilic substitution reactions. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research use only.
Alkyne-PEG2-iodide is a crosslinker containing a propargyl group and an iodine group. The propargyl group can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry reactions. Iodine (I) is a very good leaving group for nucleophilic substitution reactions. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research use only.
Medium-Salt Active Nuclease High Quality (M-SAN HQ) is a Bioprocessing Grade nuclease developed for removal of both single and double-stranded DNA and RNA at the physiological salt conditions most often used in bioprocessing and biomanufacturing workflows. M-SAN HQ allows you to directly replace Benzonase without changing your workflow.
This novel, nonspecific endonuclease is active over a broad pH range and displays optimum activity at salt concentrations between 125 – 250 mM. Due to its excellent performance at physiological conditions, M-SAN HQ can be used directly in the cell medium or the harvested supernatant without buffer adjustments. This makes M-SAN HQ ideal for the manufacturing of fragile vectors such as lentiviruses and retroviruses.
M-SAN HQ can be directly used in medium without buffer adjustments The high activity of M-SAN HQ at standard cell medium conditions leads to improved DNA clearance compared to other commonly used nucleases. In the data (see Figure 6), an over 2-fold reduction in residual DNA was achieved.
Optimal activity at physiological salinity and pH makes it ideal for DNA removal from mammalian cell media. (see Figures)
The high activity of M-SAN HQ at the physiological salinity and pH found in standard cell medium conditions leads to improved DNA clearance compared to commonly used nucleases.
M-SAN HQ is uniquely formulated to excel in physiological pH conditions, offering high performance at the commonly used cell media pH of 7.4.
While other nucleases often require more alkaline environments, M-SAN HQ stands out for its adaptability and effectiveness in cell media. It’s the nuclease that truly aligns with your bioprocessing needs. (See Figure section)
The M-SAN HQ ELISA Kit confirms the removal of M-SAN High Quality in bioprocessing and biomanufacturing applications with high accuracy:
Shelf life: 18 months
Fast: 1.5 – 2 hours
Sensitive – Quantification range: 0.12 – 7.5 ng/ml
Accurate
Flexible – 96-well plate in 8-well strip format
Medium-Salt Active Nuclease High Quality (M-SAN HQ) is a Bioprocessing Grade nuclease developed for removal of both single and double-stranded DNA and RNA at the physiological salt conditions most often used in bioprocessing and biomanufacturing workflows. M-SAN HQ allows you to directly replace Benzonase without changing your workflow.
This kit provides a rapid method for the isolation and purification of DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE DNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The purified DNA is of high yield and integrity and is free of inhibitors, ready for use in a number of downstream applications including qPCR, mutation screening, microarray analysis, sequencing, single nucleotide polymorphism (SNP) and short-tandem repeat (STR) genotyping. The protocol can be completed in as little as 1 hour.
Figure 1 / 4
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| Kit Specifications | |
| Maximum Column Binding Capacity (gDNA) | 10 μg |
| Maximum Loading Volume Per Spin Column | 650 μL |
| Size of DNA Purified | All sizes > 80 bp |
| Maximum Amount of Starting Material | 5 sections < 20 µm thick paraffin slices 25 mg of unsectioned block |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 47400 (50 preps) |
|---|---|
| Digestion Buffer A | 25 mL |
| Buffer RL | 30 mL |
| Wash Solution A | 38 mL |
| Elution Buffer B | 15 mL |
| Proteinase K | 12 mg |
| RNase A | 1 tube |
| gDNA Purification Micro Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
Permagen’s Dual Volume 0.2 mL PCR Strip separator uses the same magnet configuration as our MSR812 rack above, however, when using the opposite side, the magnets are positioned towards the bottom of the tubes allowing low elution’s to be achieved. Angled tube holes allow clean area at the front of the well for clean, fast aspiration without the concern of disturbing bead pellets Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic beads
MSRLV08
Maximum – .2 mL
Minimum – 5 µL
Permagen’s Dual Volume 0.2 mL PCR Strip separator uses the same magnet configuration as our MSR812 rack above, however, when using the opposite side, the magnets are positioned towards the bottom of the tubes allowing low elution’s to be achieved. Angled tube holes allow clean area at the front of the well for clean, fast aspiration without the concern of disturbing bead pellets Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic beads
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