Introduction

The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA and miRNA from 0.2-0.6ml serum and plasma
Applications
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type
Sample amount
Yield
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column

Principle

HiPure RNA technology simplifies total RNA isolation. The sample material is denatured in Lysis Buffer. The protein is then precipitated by Protein Precipitation Solution and pelleted by centrifugation. After the removal of protein the binding conditions for nucleic acids are adjusted by adding isopropanol. Total nucleic acids are bound to the column. Optionally, DNA can be removed by an on-column DNase digest. The remaining nucleic acids are washed and eluted with minimal amounts of RNase-Free water.

Kit Contents

Contents	R431402	R431403
Purification Times	50 Preps	250 Preps
HiPure RNA Mini Columns	100	2 x 250
2ml Collection Tubes	100	2 x 250
Buffer CFL	6 ml	30 ml
Buffer CFP	1.8 ml	10 ml
Buffer RWC	20 ml	80 ml
Buffer RW2*	20 ml	2 x 50 ml
RNase Free Water	10 ml	60 ml

Storage and Stability

The kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

LCD touch screen floor standing large capacity refrigerated centrifuge

Features

1. Microprocessor control, High resolution LCD display with touch screen functions. Parameters (speed, RCF, time)in running can be edited and changed.

2. Program memory up to 100 kinds. It can store all the parameters automatically in operation, and can use directly when start up next time.

3. Hold function

4. 10 Acceleration & 10 deceleration soft brake system.

5. Brush-less frequency motor with simpler construction, more reliable performance, longer life and quietly running.

6.  Automatic lid lock, automatic door opening and closing with automatically rotor lid opening.(Optional)

7. super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.

8.  Adapters are available by experiment requirements.                          

9. 3 tiers protection steel cover, safe and reliable.

10. CE EMC IEC 61010 certificate.

GL12 Technical Parameter：

Max. Speed	12000rpm
Max. RCF	22650×g
Max. Capacity	4×1000ml
Timer	1~9h59min
RPM/RCF Convert	Yes
Noise (dB)	≤ 60
Temperature Range	-20~40℃
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	±1℃
Voltage(V/Hz)	AC 220V/110V  50HZ/60HZ
Size (L x W x Hmm)	780×675×830mm
Net Weight(Kg)	260KG
Certificates	CE,ISO & Calibration report are available

Matched Rotors for GL12

Order No.	Rotor No.	Max Speed (rpm)	Max Volume(ml)	Max.RCF（×g）
L12-1	Angle Rotor	12000	6×250/300ml	22650
L12-2	Angle Rotor	8000	6×500ml	11740
L12-3	Swing Rotor	4000	4×1000ml	4060
L12-4	Microplate Rotor	4000	4×4×96well	2940
L12-5		4000	2×4×96well	2490
L12-6	Angle Rotor	10000	4×500ml	16110

Model:
GL12

Brand:
Yingtai

Code:
8421199090

Download:
Brochure of GL12 centrifuge touch screen.pdf

DBCO-PEG6-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The hydrophilic PEG spacer increases the water solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG6-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The hydrophilic PEG spacer increases the water solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.