Thermal Combo Block 1.5 mL 0.2 mL PCR Chiller
SKU: CB0215
Permagen’s Thermal Combo Block 1.5 mL, 0.2 mL PCR was designed as a temperature control device that can be used manually or on a liquid handling robot
SKU: CB0215
Permagen’s Thermal Combo Block 1.5 mL, 0.2 mL PCR was designed as a temperature control device that can be used manually or on a liquid handling robot
Intended Usage:
For cultivating fastidious bacteria.
Principle:
Tryptone, peptone, and yeast extract multivalent powder provides a nitrogen source, vitamins, and growth factors; sodium chloride to maintain osmotic balance; glucose carbon source; dipotassium hydrogen phosphate as a buffering agent.
Formulation（per liter）:
Tryptose 20.0g
Glucose 2.0g
Dipotassium hydrogen phosphate 2.5g
Sodium chloride 5.0g
Final pH 7.3±0.2
How to use：
Suspend 29.5g in 1L of purified water. Heat with frequent agitation to dissolve the powder.
Sterilize at 121℃ for 15 minutes
Storage:
Keep the container tightly closed. Store in a cool, dry place, away from bright light. The storage period is 3 years.
Specification: 500g/bottle
500g
Different-sized amplicons from 3 ng of a DNA plasmid were amplified. The use of Taq DNA polymerase resulted in clean and high yield of products, as analysed after PCR on a 0.8% agarose gel.
A fragment (64 bp) of the human blood-coagulation factor IIa (F2) was amplified from 20 ng, 2 ng, 200 pg and 20 pg of a human genomic DNA extract. The same experiment was performed in parallel using the Taq DNA polymerase mix from another well-established and known supplier. PCR products were subsequently analysed on a 2.5% agarose gel.
Taq 2x PCR Master Mix – ready to use mix simplifies your PCR setup. Only primers and template need to be added as the mix contains all copmponents for a successful and reliable PCR. This ensures reproducible results, significantly reduces set-up times and the risk of pipetting errors. The robustness of PCR performance allows the application of this mix in a wide range of PCR amplifications.
