ArcticZymes RNA to DNA Ligase (ArcticZymes R2D Ligase™) is the first ligase on the market that is able to ligate DNA to 5’-phosphorylated ends of RNA in the presence of a DNA template positioning the joinable ends. With its unique substrate specificity, ArcticZymes R2D Ligase allows the development of new technologies in molecular biology research, diagnostics, and manufacturing.
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ArcticZymes RNA to DNA Ligase (ArcticZymes R2D Ligase™) is the first ligase on the market that is able to ligate DNA to 5’-phosphorylated ends of RNA in the presence of a DNA template positioning the joinable ends. With its unique substrate specificity, ArcticZymes R2D Ligase allows the development of new technologies in molecular biology research, diagnostics, and manufacturing.
Key Features:
Novel substrate specificity
RNA to DNA ligation
High purity
Detergent free
Efficient ligation of DNA to 5’-phosphorylated ends of RNA in the presence of a DNA template positioning the ligatable ends of DNA and RNA
ATP dependent dsDNA ligase
Utilizes Mg2+ or Mn2+
Suggested Applications:
RNA capturing
In vitro transcription
NGS library prep
Transcriptome amplification
miRNA detection/analysis
RNA splint ligation
Other Products
[CD5000] SMOChem™ dCTP Solution – Sodium Salt (100 mM), 25ml
Product Info
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Product Info
Description
Ultrapure dCTP supplied as sodium salt in purified water (pH 8.5).
Features
Ideal for PCR amplification and cDNA synthesis
Nuclease and ribonuclease free
Applications
PCR
RT-PCR
Storage
-20°C for 36 months
Document
Ultrapure dCTP supplied as sodium salt in purified water (pH 8.5).
Cytokeratin 19 (CK19) forms intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue and provides mechanical support. Anti-Cytokeratin 19 stains epithelia and epithelial malignancies such as carcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Cytokeratin 19 is a useful marker for distinguishing hepatocellular carcinoma from intrahepatic cholangiocarcinoma. This differentiation is improved when stained in combination with Cytokeratin 7, CAM5.2l, Ber-EP4/MOC31, HepPar1 and TTF1. Cytokeratin 19 staining can also be used to recognize thyroid papillary carcinomas.
A rapid, high performance dye-terminator removal process based on the paramagnetic bead technology. The paramagnetic bead format requires no centrifugation or filtration and is easily performedmanually or fully automated for high throughput dye-terminator removal. Compared to similar systems, this product produces sequences with longer Phred 20 read lengths and higher signal intensities than any other purification technology.
Details
Specifications
Features
Specifications
Main Functions
Removal of free fluorescent dye from sequencingsolution (Replace Beckmen or agencourt CleanSeq)
Applications
Automated sequences
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
DNA doped with fluorescentdye
Sample amount
5μl
Recovery
90%
Elution volume
≥25μl
Operation time
≤50 minutes
Principle
The CleanSeq method contains magnetic particles in an optimized binding buffer to selectively capture sequencing extension products. The protocol can be performed directly in the thermal cycling plate. Unincorporated dyes, nucleotides, salts and contaminants are removed using a simple washing procedure. The purification procedure is amenable to a variety of automation platforms since it requires no centrifugation or vacuum filtration.
Advantages
High recovery – up to 90%
High throughput – using magnetic beads purification technology
CleanSeq Beads should be stored at 2-8°C upon arrival and is stable up to 6 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. Mix CleanSeq Beads well before using. The reagent should appear homogenous and consistent in color.
DO NOT FREEZE.
Document
A rapid, high performance dye-terminator removal process based on the paramagnetic bead technology. The paramagnetic bead format requires no centrifugation or filtration and is easily performedmanually or fully automated for high throughput dye-terminator removal. Compared to similar systems, this product produces sequences with longer Phred 20 read lengths and higher signal intensities than any other purification technology.
