Introduction

HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 80bp-15kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

Note: This kit can only recover 200mg of gel at most. If it is larger than 200mg, the column may be blocked. This column can only recover 5μg of DNA at most.

Details

Specifications

Features	Specifications
Main Functions	Recover DNA fragments >100bp from agarose gel(<0.2g), purification of DNA from PCR or enzymatic reaction solution solution
Applications	PCR, NGS, labeling, ligation and enzyme digestion, etc.
Purification method	Micro spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Agarose gel, PCR products, enzyme products
Sample amount	Agarose gel: ≤200mgDNA products: ≤300µl (≤5µg)
Recovery	100-5000bp：85%, 5-10kb：60-70%
Elution volume	≥10μl
Time per run	≤30 minutes（1-24 samples）
Liquid carrying volume per column	800µl
Binding yield of column	20µg

Principle

The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Advantages

• High recovery efficiency – up to 80% DNA recovery
• Low elution volume – elution volume can be as low as 10μl
• General – recover DNA fromgel or enzyme-driven reaction solutions such as PCR
• Fast – isolation can be completed in 10-15 minutes by column gel method

Kit Contents

Contents	D211002	D211003
Purification Times	100 Preps	250 Preps
Buffer GDP	60 ml	125 ml
Buffer DW1	40 ml	90 ml
Buffer DW2	20 ml	50 ml
Elution Buffer	20 ml	30 ml
HiPure DNA Micro Columns	100	250
2 ml Collection Tubes	100	250

Storage and Stability

The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve

HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 80bp-15kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

Note: This kit can only recover 200mg of gel at most. If it is larger than 200mg, the column may be blocked. This column can only recover 5μg of DNA at most.

Description

Description

Our SNPsig® kits use our own proprietary genotyping method to enable the identification of SARS-CoV-2 variants of concern. These products can be used on any real-time PCR machine using familiar protocols, whilst resulting in exceptional genotyping data.

Positive control templates for wild-type and variants are supplied in every kit to make data interpretation simple.

Our SNPsig® technology provides an alternative to sequencing as well as S gene target failure (SGTF) that enables scientists to analyse and monitor these specific genomic mutations.  Our kits can provide a pivotal role in screening for SARS-CoV-2 variants for the purpose of genomic surveillance and studies.

For the detection of the SARS-CoV-2 variants with the 20I/501Y.V1, VOC-21FEB-02 and variants carrying the E484K mutation
Rapid detection of specific detection profiles
High priming efficiency
Sensitive to < 100 copies of target Positive copy number standard curve for quantification Accurate controls to confirm findings 96 reactions, includes master mix

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request