The Qubit assay tubes are thin-wall polypropylene tubes (500 µl) for use with the Qubit Fluorometer. High-quality of the tubes guarantee the accurate measurement of different types of samples such as DNA (dsDNA, ssDNA), RNA and protein.
Detail
Assay Tubes (Qubit Fluorometer)
The Qubit assay tubes are thin-wall polypropylene tubes (500 µl) for use with the Qubit Fluorometer. High-quality of the tubes guarantee the accurate measurement of different types of samples such as DNA (dsDNA, ssDNA), RNA and protein.
Qubit tubes from the same lot should be used for the experiments for optimal result. 500 tubes per package.
Features
High-quality: Accurate measurement of different types of samples
Cost cutting: Half price as compared to other vendors
Specifications
Shipping condition
Room temperature
Type
Assay tube (for Qubit fluorometer)
For use with (equipment)
Qubit fluorometer
Storage
Store at room temperature
Package
500 tubes
Qubit is a registered trademark of ThermoFisher Scientific.
Other Products
DBCO-PEG8-PFP ester
Product Info
Document
Product Info
DBCO-PEG8-PFP ester is a PEG reagent comprising a DBCO group which can react with azides for copper-free Click Chemistry reactions. The PFP ester is an active ester which can be used to react with amine groups. PFP esters have been are stable compounds and are less susceptible to undergo hydrolysis. PEG8 linker increases the water solubility of a compound in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
DBCO-PEG8-PFP ester is a PEG reagent comprising a DBCO group which can react with azides for copper-free Click Chemistry reactions. The PFP ester is an active ester which can be used to react with amine groups. PFP esters have been are stable compounds and are less susceptible to undergo hydrolysis. PEG8 linker increases the water solubility of a compound in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Aflatoxin is the most common food toxin that is harmful to human and animal health. The most frequent aflatoxins are B1, B2, G1, and G2, which can affect the body through respiratory, mucosal, or cutaneous routes, causing an excessive inflammatory response. Aflatoxin can infect crops during their growing stages or even after they are harvested. It mainly targets the liver and can impair the effectiveness of immunization in children, increasing the risk of infection. Aflatoxin detection and quantification in food and feed is a critical part of food and feed safety concerns.
Document
Aflatoxin is the most common food toxin that is harmful to human and animal health. The most frequent aflatoxins are B1, B2, G1, and G2, which can affect the body through respiratory, mucosal, or cutaneous routes, causing an excessive inflammatory response. Aflatoxin can infect crops during their growing stages or even after they are harvested. It mainly targets the liver and can impair the effectiveness of immunization in children, increasing the risk of infection. Aflatoxin detection and quantification in food and feed is a critical part of food and feed safety concerns.
PACE (PCR Allelic Competitive Extension) genotyping chemistry is a homogeneous, PCR-based allele-specific technology for the analysis of DNA sequence variants, most commonly SNPs (Single Nucleotide Polymorphisms) and Indels (insertion / deletions).
PACE genotyping chemistry is comprised of two parts:
PACE Genotyping Assay: two allele-specific forward primers and one common, reverse primer. The allele-specific forward primers each have different 3′ terminal bases reflecting the target variant, and a unique 5’ tail sequence which is incorporated as part of the fluorescent signal mechanism.
PACE Genotyping Master Mix: containing all remaining components required for PCR and the generation of fluorescent signals. PACE Genotyping Master Mix contains a novel, universal, fluorescent reporting cassette to produce machine-readable fluorescent signals (FAM and HEX) corresponding to the assay genotypes.
When combined with sample DNA, these components create a PACE Genotyping Reaction, as illustrated in the figure below.
We have extensive knowledge and experience in assay design, especially when it comes to allele-specific PCR. PACE Genotyping Assays are available to purchase either Validated and Unvalidated. Validated assays require customer DNA to validate and optimise, for guaranteed performance. Unvalidated assays are designed in silico and supplied untested.
REQUIRED COMPONENTS
qPCR machine or Thermocycler + Fluorescent plate reader
PCR plate or equivalent and appropriate optically clear seal
Template DNA
PCR-grade water
PACE Genotyping Master Mix or PACE 2.0 Genotyping Master Mix
STEPS TO YOUR PACE GENOTYPING ASSAY DESIGN
Place your order on this page. Our support team will contact you by email.
Fill out an Assay Design Template form with all the information we need to process your custom PACE Genotyping Assay order. We will email you a copy of the template when we first contact you, or your can download a copy here.
Using the information you provide us, we will create your PACE Genotyping Assay designs, order the oligos, and send you design sequences.
Once we receive the oligos, we assemble the assay(s) and then ship an aliquot to you (unvalidated) or test on your DNA samples before shipping the aliquot to you (validated).
