Overview

• Can be used for the clean up of both RNA and DNA from enzymatic reactions, labeling etc.
• Purifies all sizes of RNA, from large mRNA down to microRNA (miRNA)
• Purifies all sizes of DNA, from small PCR products to plasmids to genomic DNA
• Removes endotoxins for transfection of injection ready RNA or DNA
• Rapid and efficient spin-column format (20 minutes)
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s CleanAll DNA/RNA Clean-Up and Concentration Micro Kit provides a rapid method for the purification, cleanup and concentration of RNA or DNA from different isolation methods or upstream applications.   The kit can be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions. 

The CleanAll Kit purifies RNA from phenol/guanidine-based protocols or from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. Furthermore, endotoxins can be removed from previously purified RNA solutions.

The kit can be used to clean up DNA from digestions, ligations, PCR reactions, labeling reactions, DNA modification reactions and staining. The kit also provides a protocol for the rapid removal of endotoxins from previously purified DNA down to 0.1 EU/µg DNA or less.

Purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA).

Purify all sizes of DNA, from small PCR products, native or linearized plasmids, to genomic DNA. Using an alternative protocol, even oligonucleotides and smaller DNA fragments can be purified.

Details

Supporting Data

Figure 1 / 2

Previous

Next

Click for expanded view

Kit Specifications
Column Binding Capacity	50 μg RNA 10 μg DNA
Maximum Column Loading Volume	600 μL
Minimum Elution Volume	20 μL
Time to Complete 10 Purifications	20 minutes
Size of RNA Purified	All sizes, including small RNA (<200)
Size of DNA Purified	≥ 100 bp*
Average Recovery	≥90% for RNA ≥90% for DNA 100 bp to 10 kbp ≥75% for DNA ≥ 10 kbp

* Applicable to smaller fragments or oligonucleotides with alternative protocol

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.

Component	Cat. 23800 (50 preps)
Binding Buffer H	60 mL
Wash Solution K	27 mL
Elution Buffer L	15 mL
Micro Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Introduction

Usages:

For differentiation of enterobacteriaceae.

Principle:

Peptone, beef extract powder and yeast extract powder provide nitrogen, vitamins, minerals; lactose, dextrose into fermentable sugars, which produce acid when measured by the phenol red indicator, acid yellow, basic red; thiosulfate sodium can be reduced to some bacteria to hydrogen sulfide, to produce a black iron sulfide and iron salts; sodium chloride to maintain osmotic equilibrium; agar as medium coagulant.

Formulation(per liter):

Peptone 20g

Beef extract powder 3g

Yeast extract powder 3g

Lactose 10g

Dextrose 1g

Sodium chloride 5g

Ferric ammonium citrate 0.5g

Sodium thiosulfate 0.5g

Agar 12g

Phenol red 0.05g

Final pH 7.4 ± 0.2

How to use:

1.Suspend 54.5g in 1L of distilled water , stirring heated to boiling ,autoclave at 121 for 15 minutes.

2.Diluted and treated samples.

Quality control:

Item	The name and number of strain	Growth	Colony Color
1	Escherichia coli ATCC25922	Good	A/A
2	Proteus CMCC (B) 49027	Good	K/A
3	Salmonella typhimurium CMCC (B) 50115	Good	K/A

“A”Acid , “K” Alkality

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

500g

Introduction

HiPure DNA Clean Up 96 Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

Details

Specifications

Features	Specifications
Main Functions	Recover DNA fragments between 60bp-10kbp from PCR, Reactions, crude DNA using 96 well Bind Plate
Applications	PCR, sequencing, labeling reactions, ligations and restriction digestion, etc.
Purification method	96 well Bind Plate
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	PCR product, enzymatic reaction
Sample amount	Appropriate
Recovery	≥80%
Elution volume	≥75μl
Time per run	≤60 minutes
Liquid carrying volume per column	800µl
Binding yield of column	20µg

Principle

The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Advantages

• High recovery efficiency – ≥80% DNA recovery
• Fast – isolation can be completed in 60 minutes

Kit Contents

Contents	D212201	D212202	D212203
Purification Times	1 x 96 Preps	4 x 96 Preps	20 x 96 Preps
Buffer GDP	120 ml	400 ml	4 x 400 ml
Buffer DW2	20 ml	100 ml	3 x 100 ml
Elution Buffer	20 ml	20 ml	30 ml
HiPure DNA Plate	1	4	20
2.2ml Collection Plate	1	4	20
1.6 ml Collection Plate	1	4	20
0.8 ml Collection Plate	1	4	20

Storage and Stability

The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.

HiPure DNA Clean Up 96 Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.