The Qubit assay tubes are thin-wall polypropylene tubes (500 µl) for use with the Qubit Fluorometer. High-quality of the tubes guarantee the accurate measurement of different types of samples such as DNA (dsDNA, ssDNA), RNA and protein.
Detail
Assay Tubes (Qubit Fluorometer)
The Qubit assay tubes are thin-wall polypropylene tubes (500 µl) for use with the Qubit Fluorometer. High-quality of the tubes guarantee the accurate measurement of different types of samples such as DNA (dsDNA, ssDNA), RNA and protein.
Qubit tubes from the same lot should be used for the experiments for optimal result. 500 tubes per package.
Features
High-quality: Accurate measurement of different types of samples
Cost cutting: Half price as compared to other vendors
Specifications
Shipping condition
Room temperature
Type
Assay tube (for Qubit fluorometer)
For use with (equipment)
Qubit fluorometer
Storage
Store at room temperature
Package
500 tubes
Qubit is a registered trademark of ThermoFisher Scientific.
Other Products
Cat.# 20106S, 20106L: Size range 450-750 bp (ideal for NGS library size selection)
Product Info
Document
Product Info
The series of DNA Size Selection Kits (Magnetic Beads) were developed for DNA size selection using magnetic beads. A total of 11 kits are available, with different selection ranges spanning from 50 bp to over 10 kb. The kits provide a simple and quick approach for the enrichment of a specific range of DNA fragments. The kit workflow allows double-sided or single-sided size selection for specific size cutoffs.
Gel images of different ranges of size selection. Sheared human genomic DNA was used as input.
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DNA size selection is a selective capture of DNA fragments of a specific range of size for next-generation sequencing (NGS) library preparations, PCR, ChIP assay, DNA ligations, endonuclease digestions, adapter removal, and other genomics and molecular biology applications. DNA size selection is preferred after NGS library prep in most of the cases. The NGS library preparation is related to the quality of the sequencing data. Precise NGS library size selection can increase sequencing efficiency, improve data quality, and reduce costs.
There are two types of sequencing technologies: short-read sequencing and long-read sequencing. Short-read sequencing uses DNA libraries that contain small insert DNA fragments of similar sizes, usually several hundred base pairs. The sequencing efficiency can be improved if the DNA size selection is in the right range. Cat.# 20104S and 20104L are the best kits for NGS library size selection of illumina paired-end 100 (PE100) sequencing with 100-200 bp library inserts; Cat.# 20105S and 20105L are the best kits for NGS library size selection of illumina paired-end 150 (PE150) sequencing with 150-300 bp library inserts; and Cat.# 20106S and 20106L are the best kits for NGS library size selection of illumina paired-end 300 (PE300) sequencing with 300-600 bp library inserts.
Long-read sequencing uses a large DNA fragment as input and makes very long reads. Usually, library size selection is preferred to remove smaller fragments. Cat.# 20110S and 20110L are the best kits for long-read sequencing size selection with DNA sizes >5 kb, and Cat.# 20111S and 20111L are the best kits for long-read sequencing size selection with DNA sizes >10 kb.
The magnetic beads technology uses paramagnetic particles, also known as SPRI (Solid Phase Reversible Immobilization) beads, to bind DNA reversibly and selectively. DNA fragments can be size-selected and purified by changing the properties of the magnetic beads or SPRI beads. The magnetic beads can easily separate the beads-binding DNA from the contaminants and unwanted components in the samples. The samples after DNA size selection are free of contaminants such as buffer components, enzymes, proteins, salts, dNTPs, primers, and adapters. Our proprietary magnetic beads reagents improve yield, selectivity, and reproducibility.
Specific DNA fragments at a certain length range can be purified simply using magnetic separation with different beads components, avoiding tedious and time-consuming gel extraction and column-based purification. The magnetic beads method is popular for common DNA size selection, including library size selection. The first beads-binding step, referred to as the right-side clean-up, removes large DNA fragments. The large DNA fragments are bound to the beads and are discarded. The desired DNA fragments in the supernatant are transferred to a new well, and new beads are added to the supernatant for the second beads-binding, referred to as the left-side clean-up. The double-size selected DNA fragments are eluted after ethanol rinsing.
DNA size selection with dual clean-ups.
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A single clean-up is needed for DNA size selection with large fragments. In this case, only the large DNA fragments are bound to the beads. The selected larger DNA fragments are eluted after ethanol rinsing.
DNA size selection with single clean-up for >5 kb and >10 kb DNA.
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Features of DNA size selection and library size selection
High specificity and high recovery of size selection
11 selection ranges are available, including 5 ranges for NGS library size selection
50-100 bp
100-200 bp
200-500 bp
250-350 bp: ideal for illumina PE100 sequencing
300-450 bp: ideal for illumina PE150 sequencing
450-750 bp: ideal for illumina PE300 sequencing
500-1000 bp
1-3 kb
1-5 kb
>5 kb: ideal for long-read sequencing
>10 kb: ideal for long-read sequencing
Fast and simple
20-min protocol
No gel purification required
No columns required
No centrifugation required
Efficient removal of contaminants and unwanted components
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
D-Fructose, D-Glucose, D-Mannose
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
4 to 80 µg of D-glucose, D-fructose or D-mannose per assay
Limit of Detection:
~ 0.7 mg/L
Reaction Time (min):
~ 30 min
Application examples:
Foodstuffs, yeast cell preparations, enzymatic hydrolysates and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Novel method
The D-Mannose/D-Fructose/D-Glucose test kit is suitable for the specific measurement and analysis of D-mannose, D-fructose and D-glucose in plant products and in acid hydrolysates of polysaccharides.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
All reagents stable for > 2 years after preparation
Only enzymatic kit available
Simple format
Rapid reaction
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Document
The D-Mannose/D-Fructose/D-Glucose test kit is suitable for the specific measurement and analysis of D-mannose, D-fructose and D-glucose in plant products and in acid hydrolysates of polysaccharides.
DNase activity in a convenient and sensitive lateral flow colormetric assay that delivers results in real time. Great for Quality Testing for DNase contamination of materials and supplies.
Attogene’s DNaseAlarm Lateral Flow test is designed for the sensitive and accurate analysis of DNAse activity in liquid samples. DNase Alarm uses a synthetic DNA substrate that attaches to the streptavidin colloidal reporter molecule (gold) using a 5’ biotin. The DNA substrate also contains a FAM molecule that enables it to be captured by the anti-FAM antibody (test line). In the absence of DNases, the DNA oligo tethers gold to the test line giving a visual test line. When DNases are present, the DNA substrate is degraded, and the gold particles can no longer be tethered to the test line thus, signal is lost. Since the cleavage of the DNA Substrate increases over time when DNase activity is present, results can be evaluated kinetically. This assay has applications for quality control testing and analysis of unit activities of DNase and DNase inhibitors. DNase’s can cause havoc in laboratories working with DNA and are important to perform routine testing.
This test can be used to rapidly and efficiently detect DNase’s in both liquid and on solid surfaces and a perfect tool for monitoring manufacturing.
Document
DNase activity in a convenient and sensitive lateral flow colormetric assay that delivers results in real time. Great for Quality Testing for DNase contamination of materials and supplies
This test can be used to rapidly and efficiently detect DNase’s in both liquid and on solid surfaces and a perfect tool for monitoring manufacturing.