50 µL filtered pipette tips for the Opentrons Flex, provided sterile in racks or blister packs (refills). Tips are clear, non-conducting polypropylene tips, free of human DNA, PCR inhibitors, RNase/DNase, pyrogens, and endotoxins. Opentrons Flex Tips and Racks are autoclavable.
50 µL filtered pipette tips for the Opentrons Flex, provided sterile in racks or blister packs (refills). Tips are clear, non-conducting polypropylene tips, free of human DNA, PCR inhibitors, RNase/DNase, pyrogens, and endotoxins. Opentrons Flex Tips and Racks are autoclavable.
DNase activity in a convenient and sensitive lateral flow colormetric assay that delivers results in real time. Great for Quality Testing for DNase contamination of materials and supplies.
Attogene’s DNaseAlarm Lateral Flow test is designed for the sensitive and accurate analysis of DNAse activity in liquid samples. DNase Alarm uses a synthetic DNA substrate that attaches to the streptavidin colloidal reporter molecule (gold) using a 5’ biotin. The DNA substrate also contains a FAM molecule that enables it to be captured by the anti-FAM antibody (test line). In the absence of DNases, the DNA oligo tethers gold to the test line giving a visual test line. When DNases are present, the DNA substrate is degraded, and the gold particles can no longer be tethered to the test line thus, signal is lost. Since the cleavage of the DNA Substrate increases over time when DNase activity is present, results can be evaluated kinetically. This assay has applications for quality control testing and analysis of unit activities of DNase and DNase inhibitors. DNase’s can cause havoc in laboratories working with DNA and are important to perform routine testing.
This test can be used to rapidly and efficiently detect DNase’s in both liquid and on solid surfaces and a perfect tool for monitoring manufacturing.
DNase activity in a convenient and sensitive lateral flow colormetric assay that delivers results in real time. Great for Quality Testing for DNase contamination of materials and supplies
Product Details
Sample Type:
Serum, Plasma, Whole Blood
Package Size:
48 Tests/Kit
Test Time:
5-20 Minutes
Target Disease:
African Swine Fever Virus
Product Name:
African Swine Fever Virus Isothermal Detetction Fluorescence Kit
Test Principle:
Nucleic Acid Detection
Target Species:
Pigs
High Light:
,
,
Product Description
Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection
Product parameters:
| Kit composition (WLRE8208KIT 48T/Pack) | |
| Composition | Content |
| E buffer | 1 mL×2 Tubes |
| B buffer | 150 μL×1Tube |
| Positive control template | 100 μL×1Tube |
| Reagents | 48T |
Product features and advantages:
| Product features and advantages | |
| Features | Advantages |
| High sensitivity | 1 copies/ml |
| Strong specificity | Specificity is superior to PCR |
| Short reaction time | 20min |
| Polymorphic reagent | Liquid, freeze-dried powder, freeze-dried ball |
| Easy to operate | Few steps of liquid dispensing; It is even possible to add only samples and amplify to get the resultsDesign of primers and probe is simple |
| Easy to store and transport | It is best preserved at -20℃, and can be stored at room temperature for up to 30 days in a cool place away from light.The freeze-dried form has a long storage time and is convenient for transportation |
| Low requirements on equipment | Applicable to Applied Biosystems 7500, QuantStudio 3/5, QuantStudio 6/7 Flex fluorescence quantitative PCR instrument;Applicable to our WL-16-III and other isothermal fluorescence detector |
Product application:
| Application | |
| Ultra-clean laboratory | African Swine Fever Virus Isothermal Detection |
| Indoor | |
Operation procedure and process:
| Operation procedure and process: |
| Take out the liquid components of the kit in advance, thaw at room temperature, and shake to mix. |
| Prepare freeze-dried reagents according to the number of samples to be tested, negative and positive controls, and add 37.5 μL E buffer to each freeze-dried reagent. |
| Select the appropriate nucleic acid extraction method and reagent to extract the sample nucleic acid |
| Add 10 μL nucleic acid template to the reaction tube (the amount of template can be adjusted to be filled with sterile water; That is, 10 μL nucleic acid template plus sterile water), 10 μL positive control template was added to positive control, and 10 μL sterile water was added to negative control |
| Add 2.5μL B buffer to each reaction tube and close the tube cap (for multiple reactions, it is recommended to add B buffer to the inside of the tube cap) |
| Thoroughly mix the reaction tube upside and down for 8-10 times. After mixing, shake (or rapidly centrifuge) the reaction liquid to the bottom of the tube and transfer it to the amplification zone. |
Performence Comparison Data:
| No. | TT value | Template concentration | Result determination |
| 1 | 4.2 | 10-1 | Positive |
| 2 | 5.5 | 10-2 | Positive |
| 3 | 10.2 | 10-3 | Positive |
| 4 | 17.0 | 10-4 | Positive |
| 5 | 12.0 | 10-5 | Positive |
| 6 | – | 10-6 | Negative |
| 7 | – | 10-7 | Negative |
| 8 | Negative control | Negative | |
Support and Services:
Livestock Disease Kit Technical Support and Services
We provide technical support and services for our Livestock Disease Kit. We are committed to helping you get the most out of your product and ensuring your satisfaction with our products and services.
If you have any questions or need technical support, please do not hesitate to contact us. We are here to help you make the most of your Livestock Disease Kit.
FAQ:
Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection
K-DATE
SKU: 700004275
50 assays (manual) / 500 assays (microplate) / 450 assays (auto-analyser)
| Content: | 50 assays (manual) / 500 assays (microplate) / 450 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | D-Lactic Acid |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 0.5 to 30 μg of D-lactic acid per assay |
| Limit of Detection: | 0.21 mg/L |
| Reaction Time (min): | ~ 5 min |
| Application examples: | Wine, soft drinks, milk, dairy products (e.g. cream, milk / whey powder, cheese, condensed milk and yogurt), foods containing milk (e.g. dietetic foods, bakery products, baby food, chocolate, sweets and ice-cream), vinegar, fruit and vegetables, processed fruit and vegetables, meat products, food additives, paper (and cardboard), cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Methods based on this principle have been accepted by DIN, GOST, IDF, EEC, EN, ISO, OIV, IFU, AIJN and MEBAK |
The D-Lactic Acid (D-Lactate) (Rapid) test kit is suitable for the rapid, specific measurement and analysis of D-lactic acid in wine, beer, juice, milk, cheese, vinegar, meat and other food products.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
See our full list of lactic acid test kits and other organic acid assay kits.
Advantages
The D-Lactic Acid (D-Lactate) (Rapid) test kit is suitable for the rapid, specific measurement and analysis of D-lactic acid in wine, beer, juice, milk, cheese, vinegar, meat and other food products.
