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AZscript™ Reverse Transcriptase (RT) is an RNA-dependent DNA polymerase used to synthesize complementary DNA (cDNA) from an RNA template.
While originating from Moloney Murine Leukemia Virus (M-MLV), AZscript RT has been engineered for increased thermostability and reduced RNase H activity.
In cDNA synthesis a higher reaction temperature can reduce RNA secondary structures and improve efficiency and specificity of the reaction.
Furthermore, a reduction in RNase H activity increases performance of the cDNA synthesis, especially for longer transcripts.
The combination of these tailored features ensures that AZscript Reverse Transcriptase provides an effective and reliable solution for various cDNA synthesis applications.
Key Features
- Increased thermostability.
- Reduced RNase H activity.
Applications
- cDNA synthesis
- Demonstrated performance in RT-qPCR
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