Introduction

This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, urine, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.

Details

Specifications

Features	Specifications
Main Functions	Extract viral RNA/DNA from non-cell/low cell content biological samples
Applications	RT-PCR，PCR，NGS
Products	Viral total nucleic acid
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Cell-free body fluid such as plasma, serum, soaking solution and tissue homogenate supernatant
Sample amount	200μl
Yield	2-10μg
Elution volume	≥30μl
Time per run	≤30 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle

This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA / RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA was finally eluted with low-salt buffer (10 Mm Tris, pH 8.0).

Advantages

• Fast – several samples can be extracted in 20 minutes by column method
• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• Safe – no phenol chloroform extraction required
• Sensitive – DNA/RNA can be recovered at the level of PG
• High yield – carrier RNA contained in the product maximize the recovery of trace nucleic acid

Kit Contents

Contents	IVD4173
Purification Times	100 Preps
HiPure Viral Mini Column	100
2ml Collection Tubes	200
PK/Carrier RNA	50 mg
Protease Dissolve Buffer	5 ml
Buffer AL	30 ml
Buffer MW1*	44 ml
Buffer MW2*	50 ml
RNase Free Water	15 ml

Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.

Experiment Data

This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, urine, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.

Introduction

This product provide fast and easy methods for purification of total DNA from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells. There is no need to use toxic phenol chloroform extraction or time-consuming alcohol precipitation. The extraction process finish in 40 minutes. Purified DNA includes genomic DNA, mitochondrial DNA, viral DNA (e.g. HBV), or DNA from other parasitic microorganisms. The obtained DNA can be directly used in PCR, viral DNA detection and other experiments.

This kit can use on manual protocol or 16/32/48 channel automated extraction system. No need to pause for the addition of binding solution (not applicable to 96/192 instruments)

Details

Kit Contents

Contents	D631001C	D631002C	D631003C
Purification Times	48	96	480
MagPure Particles	1.7 ml	3.5 ml	16 ml
Proteinase K	24 mg	50 mg	220 mg
Protease Dissolve Buffer	1.8 ml	3 ml	15 ml
Buffer MLA	30 ml	70 ml	300 ml
Buffer DW1	30 ml	70 ml	300 ml
Buffer EW	60 ml	120 ml	2 x 300 ml
Buffer MWX1	30 ml	70 ml	300 ml
Elution Buffer	10 ml	20 ml	60 ml

Prefilled Kit Contents

Product	Contents and volume	D6310-TL-06C	D6310-S-48
Proteinase K		50 mg	24 mg
Protease Dissolve Buffer		3 ml	1.8 ml
TL-Tip		12 pcs	24 pcs
Tip bottom plate/ Tip base reagent strip	Row 1/7：600μl Buffer MLA	6 plates	48 strips
	Row 2/8：600μl Buffer MWX1
	Row 3/9：600μl Buffer DW1
	Row 4/10：30μl MagPure Particles 600μl Buffer EW
	Row 5/11：600μl Buffer EW
	Row 6/12：100μl Elution Buffer

Storage and Stability

Proteinase K Powder should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these condition.

This product provide fast and easy methods for purification of total DNA from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells. There is no need to use toxic phenol chloroform extraction or time-consuming alcohol precipitation. The extraction process finish in 40 minutes. Purified DNA includes genomic DNA, mitochondrial DNA, viral DNA (e.g. HBV), or DNA from other parasitic microorganisms. The obtained DNA can be directly used in PCR, viral DNA detection and other experiments.