Biotin-PEG4-alkyne is a PEG linker containing a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays or for ligating with streptavidin proteins while terminal alkynes are used in copper (I) click chemistry with azides to form stable triazoles with a target molecule. The inclusion of a PEG linker in this molecule improves its aqueous solubility.
Biotin-PEG4-alkyne is a PEG linker containing a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays or for ligating with streptavidin proteins while terminal alkynes are used in copper (I) click chemistry with azides to form stable triazoles with a target molecule. The inclusion of a PEG linker in this molecule improves its aqueous solubility.
The HiPure Mini system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Plasmid Mini Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of tris buffer or water.
Compared to other domestic products, Magen was the first to solve the stability problem of the column. Many other brands have unstable extraction concentrations, and the longer the time, the more unstable the column becomes. However, in our test of Magen kit, the quality and yield of plasmid extraction still remain stable after 5 years’ storage. For different customers, our kits can be customized. For example, Classic type is suitable for customers with low copy or unclear plasmid types. The rapid type is suitable for customers with high copy plasmids. Compared to many other brands, the plasmid DNA extracted by Magen has a longer preservation time and more thoroughly RNA removal.
Specifications
| Features | Specifications |
| Main Functions | Isolation up to 35μg plasmid DNA from 1-5ml bacterial culture |
| Applications | Enzyme digestion, sequencing, PCR, cloning, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Conventional plasmid, plasmid less than 30KB |
| Sample amount | High copy plasmid: 1-5ml culture mediumLow copy number plasmid : 5-10ml culture medium |
| Yield | 5-35µg |
| Elution volume | ≥30μl |
| Time per run | Complete 1-24 samples in 30 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 35µg |
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
Kit Contents
| Contents | P100102 | P100103 |
| Purification Times | 100 Preps | 250 Preps |
| RNase A | 5 mg | 10 mg |
| Buffer P1 | 30 ml | 80 ml |
| Buffer P2 | 30 ml | 80 ml |
| Buffer P3 | 40 ml | 100 ml |
| Buffer PW1 | 60 ml | 140 ml |
| Buffer PW2* | 20 ml | 50 ml |
| Elution Buffer | 15 ml | 30 ml |
| HiPure DNA Mini Columns II | 100 | 250 |
| 2 ml Collection Tubes | 100 | 250 |
Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.
Experiment Data
For any technical problems or customized products, please contact us.
F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here
The HiPure Mini system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Plasmid Mini Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of tris buffer or water.
Sulfo DBCO-PEG3-NHS ester is a water soluble, click chemistry reagent which can undergo copper free click chemistry Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Sulfo DBCO-PEG3-NHS ester is a water soluble, click chemistry reagent which can undergo copper free click chemistry Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
TruScript Reverse Transcriptase is Available in Three Convenient Formats:
1. TruScript Reverse Transcriptase Kit (Cat.#54440)
This kit contains 5X RT Buffer and a vial of TruScript Enzyme Mix (200 units/µL). This enzyme can be used for reverse-transcription reactions with any user-supplied primers.
2. TruScript First Strand cDNA Synthesis Kit (Cat.#54420)
This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts).
The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and primers (oligo dT and random hexamers) for effective cDNA synthesis from total RNA transcripts.
3. TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.#54400)
This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of messenger RNA (poly A-containing transcripts).
The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and oligo dT primer for the effective cDNA synthesis from total RNA transcripts or enriched mRNA sample.
| TruScript Reverse Transcriptase | TruScript First Strand cDNA Synthesis Kit for mRNA | TruScript First Strand cDNA Synthesis Kit | |
| Kit contains only reverse transcriptase enzyme and buffer (no primers or other buffer components) | |||
| Your template is enriched mRNA | |||
| Your template is total RNA (poly A OR non-poly A-containing transcripts) | |||
| Kit contains oligo dT | |||
| Kit contains both oligo-dT primer and Random Hexamers | |||
| Kit contains reaction buffer with nucleotides and primer | with oligo-dT | with oligo-dT and random hexamers | |
| You have your own first strand synthesis primer | |||
| Your template is microRNA (using your own primers) | |||
| Cat.#54440 | Cat.#54400 | Cat.#54420 |
Figure 1 / 2
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TruScript First Strand cDNA Synthesis Kit (Cat.# 54420) – Kit Components
1. TruScript Enzyme Mix
2. 2x Reaction Mix
3. Nuclease-Free Water
4. Product Insert
Storage Conditions and Product Stability
Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers.
| Component | Cat. 54440 (10,000 units) | Cat. 54420 (50 rxns) | Cat. 54410 (50 rxns) |
|---|---|---|---|
| TruScript™ Reverse Transcriptase (200 units/ μL) | 50 μL | – | – |
| 5x RT Buffer | 1 mL | – | – |
| TruScript™ Enzyme Mix | – | 100 μL | 100 μL |
| 2x Reaction Mix | – | 500 μL | – |
| 2x Reaction Mix for mRNA | – | – | 500 μL |
| Nuclease-Free Water | 1.25 mL | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 | 1 |
