Biotin-PEG4-C1-alkyne

Description

Features of the resDNASEQ  CHO Residual DNA Quantitation kit include:

Simpler and Rapid

• Only three steps will be need for Sample Preparation and All components of the Sample Preparation Kit can be stored at room temperature.
• Only one Reagent for qPCR;
• Only 1.5 hours will be needed for the whole test.

Accurate

• Perfect amplification curve, good amplification efficiency and good precision.
• Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology.
• Limit of Detection (LOD): 0.01 fg/μL; Limit of Quantification (LOQ): 0.3 fg/μL.
• The recovery rate of different concentration samples in the linear range is between 70% and 130%.

Kit Performance

Fig 1. Only three steps will be need for Sample Preparation and only 20 minitutes will be taken for Sample Preparation.

Fig 2. Seven concentration samples of 0.3fg/μL, 3fg/μL, 30fg/μL, 300fg/μL, 3pg/μL, 30pg/μL, 300pg/μL were detected. CV of each concentration was < 30%, Regression coefficient associated with standard solutions was 0.99992, and amplification efficiency was 100.370%.

Fig 3. Five concentration samples of 0.1 fg/μL, 0.3 fg/μL, 0.5 fg/μL, 1 fg/μL and 3 fg/μL were detected, and 10 multiple wells were detected for each concentration. The CV of concentrati on values of samples with 0.3 fg/μL and above concentrations were less than 30%.

Fig 4. DNA recovery can be determined by including samples spiked with known DNA amounts which are prepared from the corresponding DNA standards. Typically, the range for this value varies from 70% to 130%.

Fig 5. Only one Reagent for qPCR MIX.

Note: Price not include shipment & duty, contact us to get full quote.
The resDNASEQ CHO Residual DNA Quantitation kit is designed for the quantification of residual DNA from CHO, in cell lines which are used for production of biopharmaceutical products. The Ducky Bio residual DNA CHO Assay, based on proven real-time qPCR technology, makes testing of residual DNA from the Chinese hamster ovary (CHO) cell line rapid, specifc. The PCR-based assay is sensitive and specific for DNA from the CHO cell line and not subject to detection of human or environmental DNA that might be introduced during sample handling. The kit was developed to meet the sensitivity requirements defined by WHO (10 ng CHO DNA per therapeutic dose).